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ARTICLE
Year : 1970  |  Volume : 18  |  Issue : 2  |  Page : 52-55

Observation on the bacteriostatic property of extract of tamarindus indica leaf (til). second series


Institute of Ophthalmology, Medical College, Calcutta, India

Correspondence Address:
B Pal Choudhury
Institute of Ophthalmology, Medical College, Calcutta
India
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How to cite this article:
Choudhury B P, Sengupta M. Observation on the bacteriostatic property of extract of tamarindus indica leaf (til). second series. Indian J Ophthalmol 1970;18:52-5

How to cite this URL:
Choudhury B P, Sengupta M. Observation on the bacteriostatic property of extract of tamarindus indica leaf (til). second series. Indian J Ophthalmol [serial online] 1970 [cited 2019 Oct 20];18:52-5. Available from: http://www.ijo.in/text.asp?1970/18/2/52/35062

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Table 4

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Table 2

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Table 2

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Table 1

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Table 1

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The effect of the extract of Tama­rindus Indica Leaf (TIL) on different bacteria is under study in the Institute of Ophthalmology, Calcutta for some time and a preliminary report on the observation of the bacteriostatic action of the extract of TIL in different con­centration on different organisms is under publication. A further study has been carried out to find out the active agent of TIL responsible for the bacteriostatic action. The extract of TIL is acid in reaction due to pre­sence of oxalic acid and it is argued that the acidity is possibly responsible for the bacteriostatic property. To de­termine the bacteriostatic action of TIL when buffered, a series of experi­ments was undertaken. As bacillus pyocyaneus is resistant to many known drugs, it was selected for the study.


  Method Top


Crude and undiluted extract of TIL (pH2 to pH3) was buffered to pH 6.4 to pH 6.8 by a solution of Sodium di­hydrogen phosphate and Disodium hydrogen phosphate and mixed sub­sequently with nutrient agar. This pH range is chosen as most of the drugs used for topical application in Oph­thalmology are neutral or near-neutral. The details of mixing are given else­where (Choudhury and Sengupta)[1]. For different concentrations of buffered extract of TIL in nutrient agar, ten sets of experiments were car­ried out with B. pyocyaneus stock cul­ture of 6 types (Pyo. I to Pyo. VI). A series of control experiments were car­ried out with the buffer in nutrient agar at pH 6.4 to pH 6.8.


  Results Top


The results of the study are tabula­ted. In all tables Pyo. I, Pyo. II, etc. indicate individual source of the B, pyocyaneus and the number of `+' signs indicates the amount of growth. One + means negligible growth, two + means moderate growth, three + means profuse growth; '-' means in­hibition of growth.

Pyo. I, Pyo. II and Pyo. III are the sources of B. pyocyaneus used in the preliminary study (Choudhury and Sengupta 1967). This table, shows that the growth of all the organisms is inhibited by a 30% mixture of buffer­ed extract in nutrient agar. The con­trol of a mixture of the buffer solution in nutrient agar shows uniformly pro­fuse growth of the organisms in all experiments.

This table shows that with 30% buf­fered extract in nutrient agar Pyo. IV growth is inhibited in 9 out of 10 ex­periments and grows moderately in 1 out of 10 experiments. Pyo. V growth is inhibited in 8 out of 10 experiments and grows profusely in 2 of 10 experi­ments. Pyo. VI growth is inhibited in 9 out of 10 experiments and grows moderately in 1 out of 10 experiments. Control shows profuse growth in all cases.

This table shows that with 25% buffered extract in nutrient agar Pyo. IV grows moderately in 1 out of 10 and the growth is inhibited in 9 out of 10 experiments. Pyo. V grows pro­fusely in 7 out of 10 and moderately in 3 out of 10 experiments. Pyo. VI grows moderately in 1 out of 10 and is inhibited in 9 out of 10 experiments. Control shows profuse growth in all cases.

This table shows that with 20% buf­fered extract in nutrient agar Pyo. IV grows markedly in 5 out of 10 experi­ments, moderately in 1 out of 10 ex­periments, negligibly in 1 out of 10 experiments and totally inhibited in 3 out of 10 experiments. Pyo. V grows profusely in 7 out of 10 experiments and moderately in 3 out of 10 experi­ments. Pyo. VI grows profusely in 4 out of 10 experiments, negligibly in 2 out of 10 experiments and totally inhi­bited in 4 out of 10 experiments. Con­trol shows profuse growth in all cases.


  Discussion Top


In a preliminary study it was claim­ed by Choudhury and Sengupta [1] that growth of Bacillus pyocy­aneus was inhibited by crude extract of TIL in concentrations of 35%, 30%, 25% and 15% in nutrient agar at pH 3 to pH 5. The present study shows that a 30% concentration of TIL buffered to pH 6.4 to pH 6.8 has also the bacteriostatic action on different strains of B. pyocyaneus. The study was performed in two series. In the first series [Table - 1], 30% concentra­tion of buffered extract of TIL in nutrient agar was observed to inhibit three strains of B. pyocyaneus. In the second series [Table - 2], a 30% concen­tration of buffered extract of TIL in­hibited the growth of B. pyocyaneus in all but four experiments. The non­inhibition of B. pyocyaneus growth in four out of thirty experiments could be due to experimental errors. In two other series of experiments with 25% [Table - 3] and 20% [Table - 4] concentration of buffered (pH 6.4 to 6.8) extract of TIL in nutrient agar the growth of B. pyocyaneus was not inhibited consistently. A series of con­trol study was done with buffer in nutrient agar at pH 6.4 to pH 6.8 which produced profuse growth of all strains of B. pyocyaneus. This shows that the buffer used in the present study does not interfere with the bacte­riostatic property of the extract of TIL in 30% concentration. It is concluded that acidity of the extract of TIL is not the primary cause of its bacterio­static property. The extract at 30% concentration must contain some in­herent bacteriostatic agent which is responsible for the observed bacterio­static.


  Summary Top


The bacteriestatic property of Tamarindus Indica (til) is indicated by a series of experiments with proper controls. The series show its effective­ness against B. Pyoeyaneous.

 
  References Top

1.
CHOUDHURY, B. P. and SENGUPTA, M. - Preliminary Observation on the Bacteriostatic Property of Tamarindus In­dica (Tamarind) Leaf Extract. J. Indian. Med. Assn., 40, 465-470, (1967).  Back to cited text no. 1
    



 
 
    Tables

  [Table - 1], [Table - 2], [Table - 3], [Table - 4]



 

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