Home About us Editorial board Ahead of print Current issue Search Archives Submit article Instructions Subscribe Contacts Login 
  • Users Online: 2406
  • Home
  • Print this page
  • Email this page

   Table of Contents      
ARTICLES
Year : 1975  |  Volume : 23  |  Issue : 4  |  Page : 14-16

Electrophoretic pattern of serum proteins in Eale's disease


Department of Ophthalmology, K.G.'s Medical College, Lucknow, India

Correspondence Address:
V B Pratap
Department of Ophthalmology, K.G.'s Medical College, Lucknow
India
Login to access the Email id

Source of Support: None, Conflict of Interest: None


PMID: 1031153

Rights and PermissionsRights and Permissions

How to cite this article:
Pratap V B, Mehra M K, Gupta R K. Electrophoretic pattern of serum proteins in Eale's disease. Indian J Ophthalmol 1975;23:14-6

How to cite this URL:
Pratap V B, Mehra M K, Gupta R K. Electrophoretic pattern of serum proteins in Eale's disease. Indian J Ophthalmol [serial online] 1975 [cited 2020 Sep 23];23:14-6. Available from: http://www.ijo.in/text.asp?1975/23/4/14/31432

Table 2

Click here to view
Table 2

Click here to view
Table 1

Click here to view
Table 1

Click here to view
The retinal perivasculitis (Eale's Disease) is thought to be a non-specific tissue response to a number of agents; tuberculo-allergic process, focal infection in skin, tooth or tonsil, certain endocrine factors involving menstrual cycle, hyperthyroidism and pituitary dyscrasias, cons­tipation and a dietary deficiency of vitamin `C' have been suggested to have some etiological significance in the causation of Eale's disease. In majority of these cases there is no particular systemic cause, hence the exact etiology of the Eale's disease remains unknown.

A large number of diseases reflect some alterations in electrophoratic pattern of serum proteins. The changes in serum proteins in patients of Eale's disease have not been well studied. It was therefore of interest to investi­gate the electrcphoretic pattern of serum pro­teins in patients of Eale's disease, so as to throw some light on the possible etiological factors in these patients.


  Material and Methods Top


Twenty seven patients of Eale's disease and 20 healthy human beings constituted the subject material for this study. Relevant clinical data were recorded and blood was collected. Erythrocyte sedemendation rate was measured in Wintrobe's tube. Serum proteins were estimated by Biuret method of Reinhold[4] as described by Varley[8]. Paper electrophoresis of these sera was performed following the technique of Henry, Golub and Sobel[2] on Whatman filter paper No.3, using barbitone buffer of pH 8.6 as described by Varley[8]. Quantitative measurement of the relative size of various protein fractions was done by dilution with 0.01N NaOH as described by Varley[8].


  Results Top


Results are shown in [Tables 1],[Table - 2].


  Discussion Top


The electrophoretic pattern of serum pro­teins in healthy human beings using the techni­que of paper electrophoresis has been studied by various workers [3],[5],[8],[7]. Our results of electrophoretic analysis of serum proteins in healthy human beings are compared to them.

Since healthy human subjects showed a minimum serum albumin content of 48.0% and maximum content of alpha-1, alpha-2, beta and gamma globulins as 5.9%, 10.7%, 15.0% and 26.9% respectively therefore in patients of Eale's disease an albumin content of less than 48.0% and alpha-1, alpha-2, beta and gamma globulin levels of more than 5.9%, 10.7%, 15.0% and 26.9% respectively were taken as abnormal. Thus the comparative evaluation of electrophoretic pattern of serum proteins in patients of Eale's disease and healthy human beings of similar age and geographical distri­bution revealed elevated levels of serum alpha-1 and alpha-2 globulins in most of the patients of Eale's disease and increase in serum gamma globulins in 33% of these cases. Since most of these patients showed elevation of various globulin fractions in their sera, therefore reduc­tion in the concentration of albumin fraction in the sera of these patients seems to be relative. Our findings differ from those of Kimura, Carriker and Hogan[9] who did not observe any consistent abnormal protein patterns in the electrophoretogrammes of serum preteins in five patients of Eale's disease studied by them.

Thus, in the present study, the elevation in serum alpha and gamma globulin fractions in most of the patients of Eale's disease points to a possible role of some immunological process, which manifests as retinal perivasculitis in the eye; an increased value of E.S.R. in most of these cases is consistent with this suggestion. The histological changes observed in the peri­vasculitis of Eale's disease which resemble very much with the histological changes observed in the blood vessels in various collagen diseases, lent further support to our suggestion. It is therefore tempting to speculate that the peri­vasculitis in Eale's disease may be in immuno­logical phenomenon.


  Summary Top


Blood collected from 27 patients of Eale's disease and 20 healthy human beings was subjected to paper electrophoresis of serum proteins and determination of E.S.R. (Win­trobe). The electrophoretic pattern of the serum proteins showed a rise in alpha-1 and alpha-2 globulins in most and in gamma globulins in about 33% of the patients of Eale's disease, the raised values of E.S.R. were encountered in about 66% of these cases. It is suggested that Eale's disease may be of immu­nological nature.

 
  References Top

1.
Duke-Elder, S., 1969, System of Ophthlamology, Vol. X, 222, Henry Kimpton, London.  Back to cited text no. 1
    
2.
Henry, R.J., Golub, O.T. & Sobel, C., 1957, Cited by Varley, 1967.  Back to cited text no. 2
    
3.
Ghai, C.L. & Kumar, S. 1963, Ind. J. Med. Res., 51, 344.  Back to cited text no. 3
    
4.
Reinhold, J.G., 1958, Cited by Varley, 1937.  Back to cited text no. 4
    
5.
Satoskar, R.S. and Lewis, R.A., 1954, Ind. J. Med. Sc., 8,446.  Back to cited text no. 5
    
6.
Sehlang, H.A. and Deris, D.R., 1958, Am. J. Med. Sc., 23,472.  Back to cited text no. 6
    
7.
Teitalbaum, J.T., Wiemer, J. and Desforges, J.P., 1959, J. Lab. Clin. Med., 53, 535.  Back to cited text no. 7
    
8.
Varley, H. 1967, Practical Clinical Biochemis­try Published by William Neinemann Medical Books Ltd. and Interscience Books Inc., New York. Fourth edition. Page 246.  Back to cited text no. 8
    
9.
Kimura, S.J., Carriker, F.R. and Hogan, M.J., 1956 Arch. Ophthal., 56, 361.  Back to cited text no. 9
    



 
 
    Tables

  [Table - 1], [Table - 2]


This article has been cited by
1 Ealesæ disease: Diagnosis and management
Das, T., Pathengay, A., Hussain, N., Biswas, J.
Eye. 2010; 24(3): 472-482
[Pubmed]
2 Ealesæ disease: Oxidant stress and weak antioxidant defence
Ramakrishnan, S., Rajesh, M., Sulochana, K.
Indian Journal of Ophthalmology. 2007; 55(2): 95-102
[Pubmed]
3 HLA typing in patients of Eales disease
Ishaq, M., Karamat, S., Niazi, M.K.
Journal of the College of Physicians and Surgeons Pakistan. 2005; 15(5): 288-290
[Pubmed]
4 Eales disease - An update
Biswas, J., Sharma, T., Gopal, L., Madhavan, H.N., Sulochana, K.N., Ramakrishnan, S.
Survey of Ophthalmology. 2002; 47(3): 197-214
[Pubmed]
5 Purification and characterization of a novel 88 kDa protein from serum and vitreous of patients with Ealesæ disease
Sulochana, K.N., Rajesh, M., Ramakrishnan, S.
Experimental Eye Research. 2001; 73(4): 547-555
[Pubmed]
6 Cellular immune response to retinal S-antigen and interphotoreceptor retinoid-binding protein fragments in Ealesæ disease patients
Saxena, S., Rajasingh, J., Biswas, S., Kumar, D., Shinohara, T., Singh, V.K.
Pathobiology. 1999; 67(1): 39-44
[Pubmed]
7 Ealesæ disease: Accumulation of reactive oxygen intermediates and lipid peroxides and decrease of antioxidants causing inflammation, neovascularization and retinal damage
Bhooma, V., Sulochana, K.N., Biswas, J., Ramakrishnan, S.
Current Eye Research. 1997; 16(2): 91-95
[Pubmed]



 

Top
 
 
  Search
 
    Similar in PUBMED
   Search Pubmed for
   Search in Google Scholar for
    Access Statistics
    Email Alert *
    Add to My List *
* Registration required (free)  

 
  In this article
Material and Methods
Results
Discussion
Summary
References
Article Tables

 Article Access Statistics
    Viewed2123    
    Printed46    
    Emailed1    
    PDF Downloaded0    
    Comments [Add]    
    Cited by others 7    

Recommend this journal