|Year : 1981 | Volume
| Issue : 4 | Page : 331-333
Paper chromatographic analysis of aminoacids in human lenses
IM Shukla, V Bhatt, VA Shinde
Department of Ophthalmology, Pt. J.N.M. Medical College, Raipur, India
I M Shukla
Department of Ophthalmology, Pt. J.N.M. Medical College, Raipur
Source of Support: None, Conflict of Interest: None
|How to cite this article:|
Shukla I M, Bhatt V, Shinde V A. Paper chromatographic analysis of aminoacids in human lenses. Indian J Ophthalmol 1981;29:331-3
|How to cite this URL:|
Shukla I M, Bhatt V, Shinde V A. Paper chromatographic analysis of aminoacids in human lenses. Indian J Ophthalmol [serial online] 1981 [cited 2020 Feb 28];29:331-3. Available from: http://www.ijo.in/text.asp?1981/29/4/331/30925
In our previous study on the estimation of aminoacids in aqueous humour in cataractus and normal eyes, it was found that aminoacids decreased as compared to normal. It was thought that this change may have a role in the genesis of cataract. Hence the present work was undertaken to study the aminoacid pattern in normal and cataractus lenses by paper chromatography.
| Material and method|| |
Total twenty cases were studied including five normal, five immature, five mature and five hypermature lenses. The removed lenses were weighted and aminoacids extracted by the solvent extraction tecknique. Both circular and ascending chromatography were done. The dried chromatograms were stained with ninhydrine for all aminoacids and for histidine with pauly's sulphanilic acid reagent which is specific for it. After elution the colour density was measured by spectrocolorimeter. Some of the circular chromatograms stained with ninhydrine were subjected to densitometric evaluation and graphs were plotted.
| Observation and discussion|| |
On quantitative estimation the concentration of all aminoacids was decreased in cataractus lenses [Figure - 1]. This decrease was more pronounced from immature to hypermature lenses. However, the concentration of cystine, alanine and phenyl alanine in immature lenses exceeded that of normal lenses, but this was followed by decrease in mature and hypermature lenses [Table - 1]. The lowered concentration of cystine and alanine in mature and cystine, alanine and tyrosine in hypermature lenses was statistically significant.
Densitometric evaluation involving all aminoacid bands in normal and cataractus lenses from circular chromatogram [Figure - 2] also remarked low density of bands in cataractus lenses in comparison to normal [Figure - 3].
As shown by Barber, the concentration of aminoacids decrease as the maturity progresses. This loss of aminoacids in later stages of cataract development may be either through the process of increase permeability of lens capsule or enhancement in the process of diffusion or by any other unknown mechanism. This loss of aminoacids is also accompanied by selective loss of the low molecular weight proteins, as confirmed by GEL filtration chromatography of soluble lens proteins in normal and cataractous lenses.
| Summary|| |
The concentration of free aminoacids in cataractus lenses decreased as this cataractus changes progressed. The lowered concentration appeared to be the effect and not the cause for cataract formation.
| References|| |
Shukla, I.M. Billore, O.P. and Shinde, V.A. 1974, Proceedings of the All India Ophthalmological Society Vol. XXXI session 34 : 1974.
Smith, I. 1969 Chromatographic and electrophoretic techniques 3rd ed. William, Heinemann Medical Books, Ltd. London Vol. 1.
Barber, C.B., 1968, Invest Ophthal. 7 : 564.
[Figure - 1], [Figure - 2], [Figure - 3]
[Table - 1]