|Year : 1982 | Volume
| Issue : 4 | Page : 399-401
Immunological study in phakolytic glaucoma at 'T' cell level
RN Misra, RP Chaturvedi, SK Saesangi
State Institute of Ophthalmology, Allahabad, India
R N Misra
State Institute of Ophthalmology, eye Hospital, Allahabad (U.P).
Source of Support: None, Conflict of Interest: None
|How to cite this article:|
Misra R N, Chaturvedi R P, Saesangi S K. Immunological study in phakolytic glaucoma at 'T' cell level. Indian J Ophthalmol 1982;30:399-401
|How to cite this URL:|
Misra R N, Chaturvedi R P, Saesangi S K. Immunological study in phakolytic glaucoma at 'T' cell level. Indian J Ophthalmol [serial online] 1982 [cited 2020 Feb 18];30:399-401. Available from: http://www.ijo.in/text.asp?1982/30/4/399/29481
Glaucoma occurring in eyes with hypermature cataract, was known since the time of Gifford. Flock et al. sub described the condition in detail and termed it as phakolytic glaucoma. He suggested it as mechanical condition caused by obstruction of angle of filtration by lens loaded macrophages. As the lens material comes out from lens capsule in this condition, and we know by now that lens is antigenic, so we studied immunological aspect of this disease at `B' and `T' cell level. The study of `B' cell level have already been reported, while at `T' cell level will be reported in the present paper by leukocyte migration inhibition test.
| Materials and methods|| |
The study was carried out in state institute of ophthalmology, Allahabad.
(1) 10 ml of fresh Blood was Collected from the patient of phakolytic glaucoma, defibrinated by shaking with glass beads, transferred to 30 ml. test tube and was mixed with equal volume of 2 %gelatin solution in phosphate buffer saline. The test tube was kept in incubator at 37° c. for 30 minutes in 30° slant position and then for 10 minutes in upright position.
(2) Leukocytes from middle buffy layer is taken out & washed 3 times with Hank's balanced salt solution by centrifuging at 400 g. for 5 minutes each time.
(3) The suspension of leukocytes was made in 0'3 ml. of parker 199 media (PH 7.2) containing 10% horse serum. The suspension was drawn into capillary tube of 1 mm. uniform internal diameter & 75 mm. length. One end of the tubes were sealed with plasticine and were centrifuged at 220 g. for 5 minutes to pack the leukocytes. Now the tubes were cut at junction of fluid & cellular level. The portion of tubes containing the cells were fixed in migration chamber containing parkar 199 media with & without antigen. The antigen was from human lens.
(4) The migration chambers were kept horizontally at 37° c. for 16-20 hrs and the area of leukocyte migration was measured by projecting the migration chamber on screen with the help of slide projector. The area migrated in test chamber (i. e. migration ch.amber with lens antigen) were compared with area migrated in control chamber (i. e. migration chamber without lens antigen). The migration index was calculated from following formula.
| Observations|| |
The study was conducted on fifteen cases of phakolytic glaucoma. In all the cases the blood was collected preoperatively. Out of fifteen cases in four cases the test was defective due to changed pH in migration chamber by lens suspension or due to other defects.
Out of eleven cases the leukocyte migration was unaffected in four cases, in six cases it was insignificantly inhibited, while only in one case some significant inhibition were seen.
| Discussion|| |
Experimental studies have shown that lens is antigenic 4, phakolytic glaucoma where lens material is liberated from its capsule may also trigger the allergic reaction at B & T cell level. We have shown in the past that there is no antilens antibody in phakolytic glaucoma excluding the immunological reaction at `B' cell levels. In the present study out of fifteen cases, in eleven cases, where test could be carried out properly, the inhibition of leukocyte by lens antigen was insignificant. Only in one case the inhibition was upto 30%. The inhibition of leukocyte less than 20% is considered insignificant. (Bloom). In our study as in all cases the inhibition was either absent or very minimal so probably there is no role of `T' lymphocytes in aetiopathogensis of phakolytic glaucoma.
| Summary|| |
The study was conducted on fifteen cases of phakolytic glaucoma. The leukocytes were separated from the blood of these patients and leukocyte migration inhibition test was done against lens antigen. The proper test was carried out in eleven cases, the leukocyte migration inhibition was insignificant in ten cases, suggesting that `T' lymphocyte are not sensitive against lens & there is no delayed hypersensitivity against lens in cases of phakolytic glaucoma.
| References|| |
Gifford, 1900, Amer. J. ophthalmol. 17; 289.
2- Flock, 1955, Arch. ophthalmol. 54 ; 37.
Misra, R. N., Rahi, A. H. S. and Morgan, G., 1977, Brit. J. Ophthalmol. 61 : 215 ;
Misra, R. N., Chandra, D. B.. Statsangi, S. K. , 1980, Proc. All Ind. Ophthalmol Cong. Manipal.
Bloom, B. R., and Glade, P. R.. 1971, in vitro mathods in cell mediated immunity., Academic press, New York.
[Figure - 1], [Figure - 2], [Figure - 3]
[Table - 1]