|Year : 1983 | Volume
| Issue : 5 | Page : 473-475
Glucose estimation in tear fluid in normal eyes and in acute mucopurulent conjunctivitis
Ajit Sinha, IM Prasad, A Rahman
Eye Deptt. Nalanda Medical College & Hospital, Patna, India
Eye Deptt. Nalanda Medical College & Hospital, Agam Kuan, Patna-800007
Source of Support: None, Conflict of Interest: None
|How to cite this article:|
Sinha A, Prasad I M, Rahman A. Glucose estimation in tear fluid in normal eyes and in acute mucopurulent conjunctivitis. Indian J Ophthalmol 1983;31:473-5
|How to cite this URL:|
Sinha A, Prasad I M, Rahman A. Glucose estimation in tear fluid in normal eyes and in acute mucopurulent conjunctivitis. Indian J Ophthalmol [serial online] 1983 [cited 2020 Feb 28];31:473-5. Available from: http://www.ijo.in/text.asp?1983/31/5/473/29525
Glucose forms an important constituent of the tear fluid. Among all the non-ionised substances in tear fluid glucose has attracted most. Variable figures of glucose content sub in the tear has been given. It has also been observed that the concentration and variations of glucose in tear and blood show some relationship. This relationship of glucose in tear fluid and blood samples have been studied in acute mucopurulent conjunctivitis. This co-relation may be effective in determining hyperglycemia by use of diabetic in the tear fluid. This method of screening out cases of diabetic in an eye clinic would be very handy.
This study was undertaken in 108 patients among which 50 normal eyes and 58 of acute mucopurulent conjunctivitis were studied.
Ridlay  gave a value for glucose in normal tear fluid 65 mgm/1.00 ml. Berselline gave 27 mgm/ 100 ml. Giardin  10.2 mgm/100 ml. Adler  gave the average value of glucose to be 2.5-4.1 mgm./100 ml.
| Material and Method|| |
A 13 micro liter capillary tube was used to collect the tear fluid. One end of this capillary tube was touched in the medial canthus over the succus lacrimalis. The fluid filled up the tube by capillarity. These capillary tubes were labeled as of normal patients or of acute mucopurulent conjunctivitis.
| Discussion|| |
In the above observation [Table - 1] it was found that the glucose in tear fluid ranged between 3.58 mgm/ 100 ml. to 4.62 mgm/ 100 ml. Adler  found the average value between 2.5-4.1 mgm/ 100 ml. The older workers 6 have given higher figures which may be accounted to be due to different sample collection and assessment methods.
(The [Table - 2]) shows marked reduction in glucose level in tear fluid in cases of acute mucopurulent conjunctivitis cases. Here the range of glucose value was between 1.60 mgm/ 100 ml. to 2.10 mgm with an average value of 1.64 mgm/ 100 ml.
This value of glucose in tear shows a marked reduction of glucose value in cases of acute mucopurulent conjunctivitis as compared to that of tear glucose value in normal eyes. One hypothesis sub is put forward to explain these low tear glucose value in acute mucopurulent conjunctivitis to be due to increased utilization of this glucose by the bacteria and the conjunctival tissue. In the infective stage probably the utilization of glucose to mixed bacterial flora of acute mucopurulent conjunctivitis is more than single bacterial infection.
The difference between the normal tear glucose value among male and female patients [Table - 3] was found to be only 0.06 mgm/ 100 ml. and hence it is not considered of any significant value.
Another set of 10 uncontrolled diabetic patients with normal healthy conjunctiva were studied. Their tear glucose and blood sugar value were like this [Table - 4].
The range of fasting blood sugar varied between 148-306 mgm/ 100 ml. Similarly the fasting tear glucose level varied between 10-25 mgm/ 100 ml. It is evident that in uncontrolled diabetic the tear glucose value also rises. But there is no co-relation between the rise of sugar glucose and tear glucose value. Proportionately the tear glucose level is higher than the blood glucose rise.
Among these patients Diastix test was also done which showed changes in its colour as the value of glucose in tear was higher. It was similar to the colour changes as in urine with i high glucose value. Thus this Diastix test was found to be a very simple procedure for routine check up of diabetic patients in the eye clinic. Thus the diabetic patients can be screened out quickly in the clinic by Diastix test as compared to lengthy process of collecting urine, testing it, or collection of blood samples and sending it for chemical analysis.
| References|| |
Davson, H., Physiology of the eye, 3rd edn.Churchil livingstone, Edinburgh & Lond. p. 417, 1972.
Ridley, F., Br. Journal of Exp. Patho., 11, 4, p. 217, 1930.
Giardini, A., and Roberts J.R.E., B.J.O., 34, 737.
Adler, H.F., Physiology of the Eye 6th Edn. C.V., Mosby & Co. London 1975, 1950.
Duke Elder, S., System of ophthalmology vol. IV Henry Kimpton, London, p. 423, 1961.
[Table - 1], [Table - 2], [Table - 3], [Table - 4]