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   Table of Contents      
ARTICLE
Year : 1983  |  Volume : 31  |  Issue : 5  |  Page : 548-549

Lactate dehydrogenase (LDH) Activity in retinoblastoma


Dept. of Ophthalmology & Pathology, Inst. of Medical Sc. BHU, Varanasi, India

Correspondence Address:
A K Rohatgi
Dept. of Ophthalmology & Pathology, Inst. of Medical Sc. BHU, Varanasi
India
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Source of Support: None, Conflict of Interest: None


PMID: 6671756

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How to cite this article:
Rohatgi A K, Singh R, Shukla P K. Lactate dehydrogenase (LDH) Activity in retinoblastoma. Indian J Ophthalmol 1983;31:548-9

How to cite this URL:
Rohatgi A K, Singh R, Shukla P K. Lactate dehydrogenase (LDH) Activity in retinoblastoma. Indian J Ophthalmol [serial online] 1983 [cited 2019 Oct 16];31:548-9. Available from: http://www.ijo.in/text.asp?1983/31/5/548/36584

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Table 2

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Table 1

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Table 1

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The present study was undertaken with the object of correlating relationship between serum LDH and aqueous LDH patients with retinoblastoma.


  Material and Methods Top


In the present study lactate dehydrogenase (LDH) was estimated in 10 cases of retinoblastoma and 10 normal cases as control. Cases of both sexes included, varying in age from 7 months to 70 years. The cases were divided into 2 groups.

Group-A : Control cases (Normal Cases).

Group-B : Retinoblastoma cases.

Group-A (Control cases)

These were the cases admitted in the Bhuwalika Eye Hospital for cataract or glaucoma. They did not have any ocular infection.

Group-B (Retinoblastoma cases)

These were the cases of retinoblastoma of varying stages who were admitted in Eye Ward for surgery, with or without Radiotherapy, or referred to the radiotherapy department where surgery was not feasible or patient refused surgery.

In all these groups only those cases who did not suffer from diseases like myacordial infraction, pulmonary embolism diseases of liver or systemic malignancy were excluded. Before proceeding further for detection of serum and aqueous LDH all these patients were subjected for detail ocular examination including oblique illumination, visual acuity with and without glasses, intraocular tension, biomicroscopic examination, ophthalmoscopic examination. Routine, investigations like TLC DLC, Hb%, Blood sugar and stool examination were done. Special investigations like X-ray of skull, X-ray orbit, X-ray optic foramina and biopsy etc.

Estimation of LDH in serum and aqueous was done by calorimetric method devised by King-Wooton (1964) in the department of pathology.


  Observations Top


LDH level in control cases (Group A):

The mean serum LDH was 139.5 ± 17.8 (I.U./litre * S.D.) and mean aqueous humour LDH was 16.80 + 10.59 (I.U./litre ± S.D.). The aqueous/serum ration was 0.12 [Table - 1],[Table - 2].

LDH level in Retinoblastoma (Group B):

The mean serum LDH level was 366.1 49.74 (1.U./litre + S.D.) and mean aqueous humour LDH was 210.0 * 23.49 (LU./litre : S.D.). The aqueous/serum ratio was 0.56 [Table - 1],[Table - 2].


  Discussion Top


Although levels of LDH have been estimated in the aqueous humour of animal [1] information regarding levels LDH in the human aqueous humour seems to be scanty. Dias et al. [2] estimated serum and aqueous LDH activity in four cases of retinoblastoma. In these serum LDH activity varied from 280 to 480 units/ 100 ml. while aqueous humour LDH activity ranged from 1800 to 3250 units/100 ml. Their lowest value in aqueous in case of retinoblastoma was 6 times more than the highest value of aqueous humour of non-malignant intraocular disorders.

Kaback and Romano [3] determined Lactic Dehydrogenase Isoenzyme in aqueous humour of retinoblastoma. They found no correlation between serum and aqueous humour LDH activity in retinoblastoma.

In present study our observation shows total LDH activity in serum as well as in aqueous is much higher in retinoblastoma cases as compared to control cases. [Table - 1]. These observations are in complete agreement with the observation of Dias et al. which are suggestive of LDH activity in aqueous & serum of diagnostic value in retinoblastoma.

The ratio of aqueous LDH concentration to serum LDH concentration was also determined. Aqueous/serum ratio was 0.56 in retinoblastoma cases while it was 0.12 in control cases. These observations also tally with that of Swartz et al. [4] who have also demonstrated aqueous/serum LDH ratio quite high in retinoblastoma group as compared to non-neoplastic group.


  Summary Top


LDH estimation in aqueous humour and serum was done in 10 normal cases and 10 cases of retinoblastoma. The LDH estimation was done by calorimetric method devised by King-Wooton [5] . Total LDH activity in serum as well as aqueous is much higher in retinoblas­toma cases as compared to control cases. P value in/0.001 for both serum and aqueous when statistically compared the retinoblastoma cases with normal cases, meaning thereby it is highly significant. The ratio of LDH concen­tration in aqueous to serum is more than 0.5 in retinoblastoma cases and less than 0.5 in normal cases.

Thus the estimation of LDH levels in aqueous humour and serum is an objective diagnostic adjunct to the diagnosis of the retinoblastoma.

 
  References Top

1.
Kuhlman, R.E., and Kaufman, H.E., Arch. Ophthalmol., 63, 41, 1960.  Back to cited text no. 1
    
2.
Dias, P.L.R., Brit. J. Ophthalmol., 55, 130, 1971.   Back to cited text no. 2
    
3.
Kabak, J., and Romano, P.E.., Brit. J. Ophthal­mol., 59, 268, 1975.  Back to cited text no. 3
    
4.
Swartz, M., Herbst, R.W., and Goldberg, N.F., Amer. J. Ophthalmol., 78, 612, 1974.  Back to cited text no. 4
    
5.
King-Wooton, Microanalysis in Medical Biochemistry 4th Edition Churchill, 1964.  Back to cited text no. 5
    



 
 
    Tables

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