|Year : 1983 | Volume
| Issue : 7 | Page : 857-859
Electrolyte pattern in experimental sugar cataracts in vitro
AG Chandorkar, MV Albal, PM Bulakh
Department of Pharmacology, Dr. VM Medical.College, Solapur, India
A G Chandorkar
Department of Pharmacology, Dr. V.M. Medical College, Solapur 413 003, Maharashtra
Source of Support: None, Conflict of Interest: None
|How to cite this article:|
Chandorkar A G, Albal M V, Bulakh P M. Electrolyte pattern in experimental sugar cataracts in vitro. Indian J Ophthalmol 1983;31, Suppl S1:857-9
|How to cite this URL:|
Chandorkar A G, Albal M V, Bulakh P M. Electrolyte pattern in experimental sugar cataracts in vitro. Indian J Ophthalmol [serial online] 1983 [cited 2020 May 26];31, Suppl S1:857-9. Available from: http://www.ijo.in/text.asp?1983/31/7/857/29686
Diabetic patients specially with uncontrolled diabetes develop many complications in the eye e.g. diabetic retinopathy and cataracts. Increased blood sugar levels for a considerable time are said to be responsible for the development of cataracts in such patients. `Sugar cataracts' like those in patients have been produced in galactosemic and diabetic rats in vivo. Therefore, we have investigated whether similar `sugar cataracts can be produced in lens culture in vitro and if so what would be the electrolyte pattern in these cataractous lenses.
| Material and methods|| |
Fresh goat lenses were exposed to varying concentrations of glucose and galactose in culture media using lens organ culture technique as described earlier.' Glucose or galactose was added to the culture media in such a way that it did not change the pH, volume, or the proportionate concentrations of the other constituents of the media. The concentrations used were those which caused an increase of 50, 100 and 200% over the normal. Thus probably simulating an increase in blood sugar level in a diabetic patient reflected probably in the same proportions in ocular tissues.
Electrolytes were estimated at 24 hours with early cataractogenesis and at 72 hours when complete opacities developed.' Sodium and potassium were estimated by flame photometer, while chlorides were estimated by the titration method using diphenyl carbazole indicator and the results were compared with those obtained with solution of known Cl -ion concentration in the 10% trichlor acetic acid. Magnesium was estimated by calorimetric method, using titan yollow indicator and calcium by titration method using calcium thymophthelein as an indicator, as described by Varley. The results were compared with a simultaneously run control.
| Observations|| |
Pretreatment with glucose and galactose hastened the development of intumescence with weight gain, opacity and early disruption of lens fibres as compared to the control by 1224 hours which is consistent with our earlier morphological observations.
The electrolyte changes at 24 and 72 hours are shown in [Table - 1][Table - 2] respectively. It can be observed that intra-lenticular sodium concentration increased very significantly, while potassium, chlorides, calcium and magnesium chlorides, concentrations showed a small but significant decrease in lenses pretreated with either glucose or galactose as compared to control. Further, though pretreatment with either glucose or galactose did not show any significant qualitative change in electrolyte pattern, galactose pretreatment did cause- a greater increase in sodium levels than seen with glucose-pretreatment. Varying the concentration of glucose or galactose to 100% or 200% over normal did not alter the electrolyte pattern significantly, however, all these pretreated lenses developed opacities and other morphological changes earlier as compared to the control. Thus it seems that pretreatment triggers off or hastens the cataractous process.
| Discussion|| |
It is obvious that increase in the concentration of either glucose or galactose in the media fluid increased the susceptibility of the lens to develop opacities. In diabetes and in galactosemia, lenses convert the intralenticular glucose and galactose into sorbital and galacitol in concentrations higher to the other sites in the body, leading to an osmotic overhydration and so giving rise to early cataracts. In lens culture also glucose and galactose is converted to its respective polyols which may attain a high intra-lenticular concentrations. As these polyols are neither efficiently metabolised nor readily diffuse out in tissue media, they lead to hypertonicity followed by osmotic swelling, weight gain and subsequent opacity and lens fibre disruption. Pretreatment with glucose and galactose hence triggers off and hastens the cataractogenic process probably through accumulation of sorbital or dulcitol. Lens culture thus can be utilized to study the effect of drugs like aldose-reductase inhibitors on sorbital pathway in vitro, and may help in understanding the mechanism of development of `Sugar Cataract'.
| Summary|| |
Alterations in electrolyte composition were studied in lens organ culture after pretreatment with glucose and galactose. Electrolytes were estimated at 24 hours with early cataractogenesis and at 72 hours with complete development of opacities. Intralenticular sodium concentration increased significantly while potassium, chlorides, calcium and magnesium decreased. Pretreatment with glucose or galactose in varying concentrations did not change this electrolyte alteration pattern, but triggered off the morphological and electrolyte changes earlier as compared to control. Lens culture can thus be used to study mechanism of development of sugar cataracts in vitro.
| Acknowledgement|| |
Authors are thankful to the Dean, Dr. V.M. Medical College, Solapur for the facilities given to undertake this work.
| References|| |
Albal, M.V., Chandorkar, AG. and P.M. Bulakh. 1981, Ind. J. Ophthalmol., 29:147.
Varlery, H, 1963, "Practical Clinical Biochemistry" 3rd Edition., William Heinemann., Medical Books. Ltd., London., P. 412, 409, 404, 364.
[Table - 1], [Table - 2]