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ORIGINAL ARTICLE
Year : 1996  |  Volume : 44  |  Issue : 1  |  Page : 15-18

Changes in the corneal Na-K ATPase levels in eyes stored in moist chamber at 4C


Department of Ophthalmology, University of Toronto, 1 Spadina Crescent, Toronto, Ontario M5S 2K6, Canada, USA

Correspondence Address:
B Devi
Department of Ophthalmology, University of Toronto, 1 Spadina Crescent, Toronto, Ontario M5S 2K6, Canada
USA
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Source of Support: None, Conflict of Interest: None


PMID: 8828300

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This report deals with a chronological measurement of Na-K ATPase enzyme activity in human and bovine corneas stored in a moist chamber at 4C. Paired human and bovine eyes were sterilized by the standard eye bank procedure and stored up to 6 days. At the desired time, the corneal endothelium was assayed for Na-K ATPase activity. The protein content of each tissue sample was also determined. In a parallel set of experiments, the viability of identical stored corneas was determined by trypan blue and alizarin red staining technique, and morphometric analysis was done to quantify the extent of the corneal endothelial damage. The human corneas showed that there was a significant progressive decrease in the Na-K ATPase activity as the storage time increased. The decrease was related to morphological endothelial damage.


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