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    Abstract
    Types
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    Methods of prepa...
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    Commercially ava...
    The technique fo...
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    Disadvantages
    Fibrin glue in o...
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CURRENT OPHTHALMOLOGY
Year : 2009  |  Volume : 57  |  Issue : 5  |  Page : 371-379
 

Fibrin glue in ophthalmology


1 Dr. Rajendra Prasad Centre for Ophthalmic Sciences, All India Institute of Medical Sciences, Ansari Nagar, New Delhi, India
2 Department of Ophthalmology, Subharti Institute of Medical Sciences, Meerut, Uttar Pradesh, India
3 Dr. Patnaik's Laser Eye Institute, New Delhi, India

Date of Submission07-Oct-2007
Date of Acceptance12-Nov-2008
Date of Web Publication14-Aug-2009

Correspondence Address:
Anita Panda
Dr. Rajendra Prasad Centre for Ophthalmic Sciences, All India Institute of Medical Sciences, Ansari Nagar, New Delhi-110 029
India
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DOI: 10.4103/0301-4738.55079

PMID: 19700876

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   Abstract 

Suturing is a time consuming task in ophthalmology and suture induced irritation and redness are frequent problems. Postoperative wound infection and corneal graft rejection are examples of possible suture related complications. To prevent these complications, ophthalmic surgeons are switching to sutureless surgery. A number of recent developments have established tissue adhesives like cyanoacrylate glue and fibrin glue as attractive alternatives to sutures. A possible and promising new application for tissue adhesives is to provide a platform for tissue engineering. Currently, tissue glue is being used for conjunctival closure following pterygium and strabismus surgery, forniceal reconstruction surgery, amniotic membrane transplantation, lamellar corneal grafting, closure of corneal perforations and descematoceles, management of conjunctival wound leaks after trabeculectomy, lid surgery, adnexal surgery and as a hemostat to minimise bleeding. The purpose of this review is to discuss the currently available information on fibrin glue.


Keywords: Blepharoplasty, cyanoacrylate glue, fibrin glue, fibrinogen, pterygium, stem cells, thrombin


How to cite this article:
Panda A, Kumar S, Kumar A, Bansal R, Bhartiya S. Fibrin glue in ophthalmology. Indian J Ophthalmol 2009;57:371-9

How to cite this URL:
Panda A, Kumar S, Kumar A, Bansal R, Bhartiya S. Fibrin glue in ophthalmology. Indian J Ophthalmol [serial online] 2009 [cited 2014 Jul 30];57:371-9. Available from: http://www.ijo.in/text.asp?2009/57/5/371/55079


Suturing is a time consuming process for which surgeons are in search of an ideal alternative. An ideal suture is one which is easy to handle, non-allergenic, affordable and does not promote infection. Besides, none of the sutures currently available fulfill the requirements of an ideal suture. To overcome these shortcomings, tissue adhesives are being increasingly used. An ideal tissue adhesive should have the following properties. [1]

  • Must allow sufficient working time before inducing firm adhesion.
  • Must have adequate tensile strength to maintain wound integrity.
  • Must be biocompatible
  • Should be clear enough to permit vision.
  • Should be permeable to fluids and metabolites to prevent necrosis.
  • Must not induce inflammation.
  • Must disappear eventually to permit healing at the interface.
  • Should not carry the risk of transferring an infectious agent.
  • Accessible and affordable
The aim of the present review is to discuss the currently used tissue adhesive at length.


   Types Top


The two basic categories of tissue adhesives are - synthetic (commonest is n-butyl-2-cyanoacrylate) and biological (fibrin glue). [1]

In addition to these two tissue adhesives, newer adhesives available for surgeons are:

  • Gelatin and thrombin products
  • Albumin and glutaraldehyde products
  • Polyethylene glycol polymers
Each of these products is unique in terms of advantages and limitations and consequently used for different indications. Of all tissue adhesives, more reports available in world literature are about cyanoacrylate and fibrin glue. In this review, we shall be discussing about fibrin glue.


   Cyanoacrylate Top


Cyanoacrylate-based glues have traditionally been the most widely used glues for ophthalmic surgery. [2] Cyanoacrylates require minimal hydration to polymerise and set. They can only be used externally because they induce inflammation. The tensile strength of the bonding is one of the highest of all glues. The major draw back of cyanoacrylate glue is that they form a solid, impermeable mass in situ . This persists as a foreign body causing inflammatory reactions like giant papillary conjunctivitis [3] and corneal neovascularization. They are also impermeable to fluids and metabolites. Though these disadvantages preclude its intraocular use, they are not very significant if the glue is applied superficially. [4]


   Fibrin Sealants Top


Fibrin glue is a blood-derived product that is absorbable, relatively easy to use, and can be kept at room temperature or in a refrigerator. Although the use of fibrin as a biologic adhesive was first introduced in 1909, it was not until 1944 that Tidrick et al . used fibrin for skin graft fixation. [5] Also it was in early forties that fibrin glue was introduced to ophthalmology to fixate penetrating corneal grafts in rabbits. [6]

Fibrin glue is a biological tissue adhesive which imitates the final stages of the coagulation cascade when a solution of human fibrinogen is activated by thrombin (the two components of fibrin glue). [7],[8],[9] Fibrin glue includes a fibrinogen component and a thrombin component, both prepared by processing plasma. It can be prepared at a blood transfusion center [10],[11],[12] or from patients own blood [13],[14],[15],[16],[17],[18],[19],[20],[21],[22],[23],[24],[25],[26] or obtained as a commercially available preparation. [27] When it is derived from individual volunteer donations, it may have a low concentration of fibrinogen. [28],[29],[30] The commercially available products are produced from pools of plasma, usually contain high yields of fibrinogen and, consequently, produce firm coagulums. Unlike cyanoacrylate glue, fibrin glue forms a smooth seal along the entire length of the wound edge and thereby provides greater postoperative comfort to the patient with fewer complications. [31]


   Mechanisms of action Top


When human tissue is injured, bleeding ensues and then ceases due to formation of a blood clot. This is the initial mechanism of natural wound closure. Clot is formed as a product of the final common pathway of blood coagulation. Fibrin glue mimics this coagulation cascade resulting in its adhesive capability. [7]

Once the coagulation cascade is triggered, activated factor X selectively hydrolyses prothrombin to thrombin. In the presence of thrombin, fibrinogen is converted to fibrin. Thrombin also activates factor XIII (present in the fibrinogen component of the glue), which stabilizes the clot, by promoting polymerization and cross linking of the fibrin chains to form long fibrin strands in the presence of calcium ions [Figure 1]. This is the final common pathway for both the extrinsic and intrinsic pathways of coagulation in vivo , which is mimicked by fibrin glue to induce tissue adhesion.

There is subsequent proliferation of fibroblasts and formation of granulation tissue within hours of clot polymerization. Clot organization is complete two weeks after application. The resultant fibrin clot degrades physiologically.


   Methods of preparation Top


Numerous techniques have been used to prepare fibrin glue, either from homologous or autologous plasma. [32],[33],[34],[35],[36],[37],[38],[39],[40] The autologous source avoids any possible risk of viral transmission. Homologous fibrin glue is prepared from donors screened like other blood products, followed by inactivation of viruses by solvent / detergent treatment. [41],[42]

The plasma is centrifuged to produce a precipitate containing fibrinogen and a supernatant containing the thrombin. The precipitate is resuspended in a small volume of the supernatant and used as the fibrinogen component. The supernatant is further treated by clotting to convert residual fibrinogen to fibrin followed by its filtration to isolate the fibrin. The resulting serum is used as the thrombin component.

The various methods of preparation are:

  1. Fibrinogen: Modified Hartman's Procedure [13]
  2. Thrombin: Armand J Quick method [32]
  3. Fibrinogen rich concentrate [25],[43],[44],[45],[46],[47],[48]
  4. Preparation during emergency need [18],[44]

   Large-scale preparation of thrombin from human plasma Top


Recently Aizawa et al . have discussed the preparation of thrombin for large scale use. [49] Simlarly, Alston et al . described another cost effective method of autologus fibrin sealant from protamine precipitated fibrinogen concentrate. [50] De Somer et al . have demonstrated the mechanical and chemical characterstics of autologus surgical glue made by mixing ultra filtered plasma with gluteraldehyde. [51]


   Commercially available fibrin glue Top


Tisseel VH Fibrin sealant (Baxter AG, Vienna, Austria) is a commercially available fibrin adhesive approved by the US Food and Drug Administration as an adjunct to hemostasis. The kit contains the following in separate vials.

1. Large Blue Bottle: Sealer protein concentrate (Human), Freeze dried, vapour treated, containing:

  • Clottable protein - 75 to 115mg
  • Fibrinogen - 70 to 110mg
  • Plasma fibronectin - 2 to 9 mg
  • Factor XIII - 10 to 50 IU
  • Plasminogen - 40 to 120 mg (microgram)


2. Small Blue Bottle: Aprotinin solution, bovine 3000 KIU /ml

3. White Bottle: Thrombin 4 (bovine), freeze dried reconstituted contains 4IU/ml

4. Large Black Bottle: Thrombin 500 (bovine), freeze dried reconstituted contains 500 IU/ml

5. Small Black Bottle: Calcium chloride solution, 40mmol/L 1+2 (Fibrin component)

3+5 (Thrombin component)- Used for slow release

4+5 (Thrombin component)- Used for rapid release

It is advisable to maintain the cold chain, i.e., to keep the temperature well regulated constantly from the time after preparation till use. Before use, the syringes containing two components of fibrin glue, namely, Thrombin (Black) and Fibrinogen (Blue) are taken out from the deep freeze and thawed to room temperature.

The fibrin sealant (Tisseel VH, Baxter Healthcare Corp, Deerfield, IL) is prepared according to the manufacturer's directions. In brief, color-coded vials (color code-Black and Blue) are warmed for several minutes in a patented fibrotherm device. The procedure requires the addition of the fibrinolysis inhibitor, Aprotinin, to the sealer protein concentrate vial followed by warming. While this solution is being stirred, the second component is prepared by injecting the contents of calcium chloride vial into the Thrombin vial (Thrombin 500 or Thrombin 4, depending upon whether an early or a delayed clot is required) which is then warmed. Only a small amount of the thrombin-calcium chloride solution is required to drive the reaction to fibrin formation. To slow the process of fibrin formation, only 0.1ml of the thrombin-calcium chloride solution is withdrawn into a disposable syringe to which 0.9ml of balanced salt solution (Acorn Inc, Decatur, IL, USA) is added to achieve a 1:10 dilution. This syringe is placed into the duploject injector along with a parallel disposable syringe containing the fibrin sealer protein and fibrinolysis inhibitor. A mixer nosecone, topped by a blunt applicator needle, is attached to the 2-syringe nozzle to facilitate mixing of the two syringe components. When the common plunger is depressed, the fibrin sealer solution and the thrombin solution are combined in the nosecone, in equal volumes, to form the resulting fibrin sealant that is directly applied to the designated tissues.

The principle behind the use of fibrin glue is taken from wound healing wherein the first phase is inflammation which involves formation of thrombus through a series of events in the coagulation cascade. An indigenous preparation Reliseal (Reliance Industries, India) is also available with similar components. All fibrin sealants in use have two major ingredients, purified fibrinogen (a protein) and purified thrombin (an enzyme) derived from human or bovine (cattle) blood. Many sealants have two additional ingredients, human blood factor XIII and a substance called aprotinin, which is derived from cows' lungs. Factor XIII is a compound that strengthens blood clots by promoting cross-linkage of fibrin strands. Aprotinin is a protein that inhibits the enzymes that break down blood clots.


   The technique for application Top


The two components of fibrin glue can either be applied simultaneously or sequentially, depending on the surgeons preference.

When simultaneous application is preferred, both the components are loaded into two syringes with tips forming a common port (Duploject syringe). When injected, the two components meet in equal volumes at the point of delivery. The thrombin converts the fibrinogen to fibrin by enzymatic action at a rate determined by the concentration of thrombin. The more concentrated thrombin solution , thrombin 500, produces a fibrin clot in about 10 seconds and the more dilute thrombin solution, thrombin 4 ,results in a clot in about 60 seconds after glue application to the surgical field. As mentioned earlier, both the extrinsic and the intrinsic mechanisms of blood coagulation are bypassed but the physiological final common pathway of coagulation is replicated. Factor XIII (present in the fibrinogen component of the glue) cross links and stabilizes the clot's fibrin monomers while aprotinin inhibits fibrinolytic enzymes, consequently resulting in a stable clot.

For sequential application, thrombin is first applied on to the area of interest, followed by a thin layer of fibrinogen. In a minute or two, coagulation starts and by two or three minutes, polymerization is complete.

Alternatively, when apposition is required between opposing surfaces, thrombin solution may be applied to one and fibrinogen to the other surface.

In all of these cases, prior to application of the glue, the surgical field must be dried meticulously. After application, the tissue is pressed gently over the glue for 3 minutes for firm adhesion. At the end of the procedure, pad and bandage is applied after instillation of antibiotic drops.


   Safety of Fibrin glue Top


Fibrin glue prepared from donor is as safe as other tested blood products. [27] Most but not all viruses can be inactivated by solvent / detergent treatment. [41],[42]

The alternative approach to ensure that fibrin glue is virus free is by preparing it from homologous fresh frozen plasma from donors in whom current tests for viral markers are negative for at least six months after the donation. [47],[48] This simple accreditation measure excludes the theoretical possibility of the donors having been in the "window period" when they donated blood or plasma. To further ensure its safety, most of the proteinaceous products are sterilized by gamma irradiation.


   Advantages Top


Fibrin glue reduces the total surgical time because time required to place sutures is saved. [6],[27],[52] The use of glue has been found to lower the risk of post-operative wound infection, contrary to conventional suturing. [52],[53],[54] This can be attributed to accumulation of mucous and debris in sutures which may act as a nidus for infection. However, there is no data available to substantiate the low incidence of post operative reaction and infection.

Mixtures of fibrin glue and antibiotics are being used for local delivery of antimicrobial activity. [53] It is well tolerated, non-toxic to the tissue wherever it is applied and has some antimicrobial activity. The smooth seal along the entire length of the wound edge results in a higher tensile strength, with the bond being resistant to greater shearing stress. Fibrin glue is also a useful adjunct to control bleeding in selected surgical patients. [54] It has a low incidence of allergic reactions. However, anaphylactic reactions following its application have been reported. [55],[56] This reaction has been attributed to the presence of aprotinin in fibrin glue.

Fibrin glue encourages the formation of adhesions when applied to contaminated tissues. Its use in infected wounds [57],[58] has been reported by two authors. This could be possible due to presence of aprotinin which possesses some antimicrobial activity. [59] Chen et al . however, reported that fibrin glue failed to demonstrate any bacteriostatic effects to either Gram-ve or Gram+ve bacteria by verifying the size of the bacterial growth inhibition. [60] They also detected minimal cytotoxic activity but this was not found to be significant clinically.


   Disadvantages Top


The major drawback to its use is the risk of transmitted disease from pooled and single-donor blood donors. [47],[48],[49],[50] The same can be minimized to a great extent by obtaining the blood from screened healthy donors. [47],[48] The safest preparation is by using the patient's own blood to prepare fibrin glue. It is expensive and autologous donation requires at least 24 hours for processing.The resultant product often has variable concentrations thereby resulting in an unpredictable performance. Moreover, tensile strength of fibrin glue has not been adequately determined and precludes quantification, being dependant on various extraneous factors also.


   Fibrin glue in ophthalmology Top


Both synthetic and biologic fibrin glues have a long history of use in ophthalmology. [5],[6] Tisseel (Baxter Healthcare Corp, Deerfield II, USA) a commercially available fibrin biosealant has been used in Europe for more than 25 years in over 9.5 million surgical procedures. [61],[62]

Conjunctival surgery

As early as 1986 the glue was used in conjunctival surgery utilizing pericardium. [63] Currently, its use has gained popularity for both conjunctival closure and transplant. [64],[65] A number of authors have tried its efficacy with favorable outcome. [66],[67] Kheirkhah et al . performed amniotic membrane transplantation using fibrin glue in eyes with refractory conjunctivochalasis and achieved a complete/smooth/significant conjunctival surface in 44-56% of eyes. [68]

In pterygium surgery

Ever since the introduction of fibrin glue in ophthalmology, its major use has been in pterygium surgery. [69],[70],[71],[72],[73],[74],[75] It is a safe and effective method for attaching conjunctival autografts for wound closure following pterygium surgery. Its use results in a shorter operating time, less post operative discomfort and inflammation.

Jiang et al . in 2008 evaluated the efficacy and safety of fibrin glue in conjunctival autograft fixation in primary pterygium compared with that of suturing. [73] They found that fibrin glue application takes significantly shorter operating time and associated with fewer post operative symptoms than a sutured graft, indicating the safety of the procedure. Studies have also demonstrated its efficacy for amniotic membrane graft fixation during pterygium surgery in terms of reduction of surgical time and post operative discomfort. [74],[75] Srinivasan et al . reported that rubbing the eye can cause graft dehiscence following pterygium surgery with fibrin glue. [76]

In strabismus surgery

Spierer et al . in 1997 carried out an experimental study using fibrin glue in strabismus surgery for conjunctival closure. [77]

An experimental study was carried out by Erbil et al . in 1991 where fibrin glue was used for conjunctival wound closure in place of classical sutures. [78] Histopathological study revealed better results with fibrin sealant than those with sutures. Biender et al . in 1996 conducted a clinical study on six patients who underwent squint surgery and concluded that conjunctival closure with fibrin glue was a good alternative to suturing the conjunctiva in strabismus surgery. [79] It also resulted in a more comfortable postoperative course.

Two comparative studies, suture versus fibrin glue, from India are reported, suggesting the efficacy of fibrin glue in conjunctival closure following strabismus surgery. [80],[81] The recent study by Erbagei et al . emphasizes the role of fibrin sealant (Beriplast) as an attractive therapeutic modality in strabismus surgery. [82] Tonelli et al . in 2004 used it in a rabbit model to find the efficacy of the glue in Faden operation. [83] Though the outcome was good in their study, they commented that in small muscle recessions, the glue was not strong enough to overcome the contractive strength of the muscle.

In Corneal surgery

a) Corneal perforation and melt

Lagoutte et al . in 1989 used the glue for perforated corneal ulcer and ulcer with impending perforation, descemetocele and extremely thinned out corneas. [84] Vrabec et al. commented that it can be used as an alternative to corneal/scleral tissue fixation in perforations smaller than 2mm. [85] According to Hick, fibrin glue and cyanoacrylate tissue adhesives are both effective in the closure of corneal perforations up to 3mm in diameter. [86] They used the glue in fixing the amniotic membrane in refractory and perforated corneal ulcers and found it to be a viable option. Fibrin glue provides faster healing and induces significantly less corneal vascularization. Further studies by Bernaur et al . in 1995, Duchesne et al . in 2001 and Sii et al . in 2005 highlighted its use in corneal melt. [87],[88],[89] Similarly, Sii et al . have emphasized its use in perforated hydrops. [90]

b) Amniotic membrane transplantation

Hick et al . in 2005 and Duchesne et al . in 2001 reported its use in amniotic membrane transplantation. [86],[88] It was found to be safe and effective in fixing the amniotic membrane to the ocular surface.

Liu et al . used fibrin glue to fix a polymethyl methacrylate ring to an amniotic membrane patch on the ocular surface as a therapeutic contact lens. [91] They found it to be effective in alleviating the patient's pain and shortening surgical time.

Kheirkhah et al . performed amniotic membrane transplantation using fibrin glue in eyes with partial limbal cell deficiency and found it to be a safe and effective procedure. [92]

Sekiyama et al . evaluated the efficacy and safety of transplantation of fibrin glue coated freeze dried amniotic membrane (FD-AM) for ocular surface reconstruction. [93] They found out that the FD-AM retained most of its biological characteristics indicating that it was safe and efficacious for ocular surface reconstruction.

c) Lamellar keratoplasty

Rosenthal et al . used a platelet/fibrinogen/thrombin mixture to fixate the lamellar corneal graft in experimental animals. [94] It has also been used successfully in lamellar graft in highly vascularized and infiltrated corneas. [95],[96],[97],[98]

d) Deep anterior lamellar keratoplasty

Narendran et al . carried out deep lamellar keratoplasty (DLK) using fibrin glue supported with overlay sutures. [99] They found it to be a time efficient and effective technique. They concluded that fibrin glue is ideally suited when both recipient bed and donor buttons are of same size and thickness.

e) Penetrating keratoplasty

Katzin in early forties introduced fibrin glue into ophthalmology and performed penetrating corneal grafts in rabbits. [6] This pioneering study was the inspiration behind various studies including those by Ignacio et al . in 2006 and Bahar et al . in 2007. [100],[101] They reported that in "Top Hat" keratoplasty, fibrin glue was mechanically more stable than suturing. They used incisional bursting pressure of 185 mmHg (range 90-300 mm Hg) for testing stability, demonstrating that fibrin glue provides faster healing and induces significantly less corneal vascularization.

f) Limbal cell transplantation

Fibrin glue has also been used effectively and safely to fix the donor limbal lenticule on the bed of the recipient in cases of limbal deficiency. As suturing the thin lenticule in limbal transplantation creates special problems related to tissue apposition, suture related inflammation, vascularization and patient discomfort to the exposed sutures on the ocular surface, the use of glue was a rational alternative. [102]

g) Epikeratophakia

Using biological adhesive, Rostron et al . carried out an experimental comparative study with that of suturing the lenticules. [103] They calculated that the operating time reduced to 50% when glue was used instead of sutures. In another experimental comparitive study using both cyanoacrylate and fibrin glue, Brittain et al . found that the bond strength was 140 gm/cm. [104] Moreover, the bond strength of both, fibrin glue and cyanoacrylate glue was similar.

h) Temporary keratoprosthesis

Uhlig et al. used the fibrin glue as an aid to stabilize temporarily sutured keratoprosthesis. [105]

Refractive surgery

a) Treating epithelial ingrowth

In the refractive arena, it is for epithelial ingrowth that the product has gained the most ground, typically in recalcitrant cases of epithelial ingrowth. The glue forms a mechanical barrier and prevents the epithelial cells from growing underneath the flap, at least until the flap is healed. The glue typically dissolves gradually over a two weeks period and by then, the epithelial surface and stromal interface show complete healing with no cells in the interface.

Anderson et al . in 2003 and Yeh et al . in 2006 suggested that additional use of fibrin glue in conjunction with debridement may be helpful in preventing recurrence of epithelial ingrowth. [106],[107] Naravaez et al . in 2006 commented that even severe progressive epithelial ingrowth may be treated successfully with a combination of mechanical debridement, flap suturing and fibrin glue application. [108]

However, the major disadvantages of the use of fibrin glue for flap reattachment is that the glue is fairly opaque when it polymerizes and as a result it is difficult to see through it to determine if there are inflammatory cells in the interface. In addition, it is expensive and requires special equipment and preparation time.

While prevention of recurrent epithelial ingrowth is the most common refractive use for fibrin glue, it also has other possible applications. It is used like a bandage contact lens or as an ocular surface bandage. However, as the most rapid visual rehabilitation is required after LASEK or Epi-LASIK and glue does not form a good optical surface for vision, rather forms a rough Band-Aid, it is not advocated in routine LASEK or Epi-LASIK surgery.

b) As a temporary basement membrane

It is being used on photorefractive keratectomy operated corneas to reduce corneal haze. [109]

c) In flap tear /traumatic flap dislocation

Usually a flap tear occurs secondary to trauma, which induces some epithelial defect. In that scenario, it adheres better to the denuded surface on or around the flap and prevents epithelial ingrowth. [107]

In glaucoma surgery

a) Conjunctival closure:

Its use as an effective method of achieving conjunctival wound closure in glaucoma surgery has been described by O'Sullivan et al . in 1996. [110]

b) Management of post operative leaking bleb:

Its successful use has been reported in the management of post trabeculectomy hypotony. A number of studies have been carried out both experimentally and clinically to prove its efficacy. [111],[112],[113]

c) In glaucoma drainage device (GDD) surgery:

It is considered as a safe substitute for some of the sutures used in GDD surgery. It does not alter intra ocular pressure (IOP) control, reduces postoperative conjunctival inflammation and reduces the surgical time. However, further studies are needed to better understand the role of the glue in GDD implantation. Vatimarki used fibrin glue intra-operatively in a series of 42 eyes subjected to GDD surgery. [114] Their results revealed that mean IOP on first post operative day was 30.5 ± 10 mm Hg which subsequently became within normal limits indicating that the intraoperative use of fibrin glue was a viable option for reducing peribulbar filtration and preventing immediate post operative hypotony after GDD surgery.

Lens surgery

Its use in cataract surgery to close the capsular perforation [115] and cataract incision [116],[117] has been tried since 1987. Bushmann in 1994 conducted both experimental and clinical studies to seal the traumatic perforation of anterior and posterior capsule by fibrin glue with successful results. [118] It has also been used for the prevention of post operative astigmatism and to seal the wound in small incision cataract surgery. [119],[120] Recently, the glue is being used to fix the haptics of IOL to the tissue in place of sutures. [121]

Vitreo retinal surgery

As early as 1988, Zauberman et al. have reported its use for conjunctival wound closure following retinal detachment surgery. [64] Mentens et al . in 2007 compared the efficacy of fibrin glue in comparison with conjunctival closure by sutures following 20 gauge needle pars plana vitrectomy in 504 eyes. [122],[123] They commented that fibrin glue offers significantly better results than suturing for closure of conjunctival wounds. In another study, Batman et al . supported the view of Mentens and suggested that in case of persistance of leaking wound following transconjunctival sutureless vitreoretinal surgery, application of fibrin glue is a better alternative over suturing. [124] Its use has also been reported in macular hole surgery. [125]

Lid and adnexal surgery

a) Lid surgery

The glue was used in eyelid surgery for fixing the free autologous skin transplants for covering skin defects and the procedure is advantageous as early fibrovascular ingrowth into the transplant is stimulated. It is also helpful in lid split procedure combined with free skin graft for severe upper eyelid entropion. In lower eyelid trichiasis, glue has been used for fixation of free autologous conjunctival transplants from the upper fornix after separation of the lashes from the posterior lamella with a lid split technique. [126],[127]

b) Lacrimal surgery

It has been used for reconstructing lacerated canaliculi, [128] in canaliculocystotomy, canaliculodacryocystorhinostomy, for the microanastomosis between canaliculi and lacrimal sac and for attaching lacrimal and nasal mucosal flaps. [129]

Plastic, reconstructive and orbital surgery

Gosalin et al . used fibrin glue to attach soft tissue in oculoplastic surgery. [130] In orbital surgery it has been used to fix the secondary orbital implant. It has also been used in plastic and reconstructive surgery. [130],[131]

Drug delivery

The suitability of existing topical fibrin glue preparations for local drug delivery is greatly limited because of the limited capacity of fibrinogen to actively bind growth factors or other therapeutic agents.


   Conclusions Top


As the use of sutures is fraught with complications , bioadhesives have emerged as a viable alternative for tissue cooptation, over the last three decades. In summary, the advantages of fibrin glue that makes it so useful in ophthalmolgy are:

  • It reduces surgical time
  • Adequate bond strength, good sealent, safe, minimal allergic or toxic reactions and minimizes bleeding
  • Easy to undo
  • Disappears eventually
  • Can plug perforations
  • Excess amount can be trimmed
  • Does not produce inflammation
However, further studies are required before fibrin glue is inducted in standard ophthalmological practice in the place of sutures.

 
   References Top

1.Forseth M, O'Grady K, Toriumi DM. The current status of cyanoacrylate and fibrin tissue adhesives. J Long Term Eff Med Implants 1992;2:221-33.   Back to cited text no. 1    
2.Trott AT. Cyanoacrylate tissue adhesives. An advance in wound care. JAMA 1997;277:1559-60.   Back to cited text no. 2    
3. Carlson AN, Wilhelmus KR. Giant papillary conjunctivitis associated with cyanoacrylate glue. Am J Ophthalmol 1987;104:437-8.  Back to cited text no. 3    
4.Tseng YC, Hyon SH, Ikada Y, Shimizu Y, Tamura K, Hitomi S. In vivo evaluation of 2-cyanoacrylates as surgical adhesives. J Appl Biomater 1990;1:111-9.  Back to cited text no. 4    
5.Tidrick RT, Warner ED. Fibrin fixation of skin transplant. Surgery 1944;15:90-5.  Back to cited text no. 5    
6.Katzin HM. Aqueous fibrin fixation of corneal transplants in the rabbit. Arch Ophthalmol 1945;35:415-20.  Back to cited text no. 6    
7.Thompson DF, Letassy NA, Thompson GD. Fibrin glue: A review of its preparation, efficacy, and adverse effects as a topical hemostat. Drug Intell Clin Pharm 1988;22:946-52.   Back to cited text no. 7    
8.Chabbat J, Tellier M, Porte P, Steinbuch M. Properties of a new fibrin glue stable in liquid state. Thromb Res 1994;15:525-33.  Back to cited text no. 8    
9.Le Guιhennec L, Layrolle P, Daculsi G. A review of bioceramics and fibrin sealant. Eur Cell Mater 2004;8:1-10.  Back to cited text no. 9    
10.Spotnize WD, Mintz PD, Avery N, Bithell TC, Kaul S, Nolan SP. Fibrin glue from stored human plasma. An inexpensive and efficient method for local blood bank preparation. Am Surg 1987;53:460-2.  Back to cited text no. 10    
11.Kram HB, Nathan RC, Mackabee JR, Klien SR, Shoemaker WC. Clinical use of nonautologous fibrin glue. Am Surg 1988;54:570-3.   Back to cited text no. 11    
12.Gammon RR, Avery N, Mintz PD. Fibrin sealant: An evaluation of methods of production and the role of the blood bank. J Long Term Eff Med Implants 1998;8:103-16.  Back to cited text no. 12    
13.Hartman AR, Galanakis DK, Honig MP, Seifert FC, Anagnostopoulos CE. Autologous whole plasma fibrin gel. Intraoperative procurement. Arch Surg 1992;127:357-9.   Back to cited text no. 13    
14.Stechison MT. Rapid polymerizing fibrin glue from autologous or single donor blood: Preparation and indications. J Neurosurg 1992;76:626-8.   Back to cited text no. 14    
15.Kjaergard HK, Weis-Fogh US, Sørensen H, Thiis J, Rygg I. A simple method of preparation of autologous fibrin glue by means of ethanol. Surg Gynecol Obstet 1992;175:72-3.   Back to cited text no. 15    
16.Alston SM, Solen KA, Broderick AH, Sukavaneshvar S, Mohammad SF. New method to prepare autologous fibrin glue on demand. Transl Res 2007;149:187-95.  Back to cited text no. 16    
17.Quigley RL, Perkins JA, Gottner RJ, Curran RD, Kuehn BE, Hoff WJ, et al . Intraoperative procurement of autologous fibrin glue. Ann Thorac Surg 1993;56:387-9.  Back to cited text no. 17    
18.Pace RM. Intraoperative preparation of autologous fibrin gel. AORN J 1995;62:604-7.  Back to cited text no. 18    
19.Isaacson G, Herman JH. Autologous plasma fibrin glue; rapid preparation and selective use. Am J Otolaryngol 1996;17:92-4.  Back to cited text no. 19    
20.Buckley RC, Breazeale EE, Edwand JA, Brzezienski MA. A simple preparation of autologous fibrin glue for skin-graft fixation. Plast Reconstr Surg 1999;103:202-6.   Back to cited text no. 20    
21.Yoshida H, Hirozane K, Kamiya A, Comparative study of autologous fibrin glues prepared by cryo-centrifugation, cryo-filtration, and ethanol precipitation methods. Biol Pharm Bull 1999;22:1222-5.  Back to cited text no. 21    
22.Komatsu F, Yoshida S. Large volume apheresis of autologous plasma and preparation of autologous fibrin glue from the plasma. Ther Apher 2001;5:12-6.   Back to cited text no. 22    
23.Buchta C, Dettke M, Funovics PT, Hφcker P, Knφbl P, Macher M, et al . Fibrin sealant produced by the CryoSeal FS System: Product chemistry, material properties and possible preparation in the autologous preoperative setting. Vox Sang 2004;86:257-62.  Back to cited text no. 23    
24.Buchta C, Dettke M, Funovics PT, Hirschl AM, Macher M, Worel N, et al . Impact of manufacturing, irradiation and filtration steps to bacterial contamination of autologous fibrin sealant. Biologicals 2004;32:165-9.  Back to cited text no. 24    
25.Shukla J, Jain P. Preparation of autologous platelet-rich fibrin glue, an effective tissue adhesive in the haematology laboratory. Indian J Pathol Microbiol 2004;47:213-4.  Back to cited text no. 25    
26.Schips L, Dalpiaz O, Cestari A, Lipsky K, Gidaro S, Zigeuner R, et al . Autologous fibrin glue using the Vivostat system for hemostasis in laparoscopic partial nephrectomy. Eur Urol 2006;50:801-5.   Back to cited text no. 26    
27.Siedentop KH, Park JJ, Shah AN, Bhattacharya TK, O'Grady KM. Safety and efficacy of currently available fibrin tissue adhesives. Am J. Otolaryngol 2001;22:230-5.   Back to cited text no. 27    
28.Dresdale A, Rose EA, Jeevanandam V, Reemtsma K, Bowman FO, Malm JR. Preparation of fibrin glue from single-donor fresh-frozen plasma. Surgery 1985;97:750-5.  Back to cited text no. 28    
29.Dresdale A, Bowman FO Jr, Malm JR, Reemtsma K, Smith CR, Spotnitz HM, et al . Hemostatic effectiveness of fibrin glue derived from single-donor fresh frozen plasma. Ann Thorac Surg 1985;40:385-7.  Back to cited text no. 29    
30.Stuart JD, Morgan RF, Kenney JG. Single donor fibrin glue for hand burns. Ann Plast Surg 1990;24:524-7.   Back to cited text no. 30    
31.Sharma A, Kaur R, Kumar S, Gupta P, Pandav S, Patnaik B, Gupta A. Fibrin glue versus N-butyl-2-cyanoacrylate in corneal perforations. Ophthalmology 2003;110:291-8.  Back to cited text no. 31    
32.Quick AJ. Hemostasis and thrombosis: a new look. Minn Med 1967;50:1333-7.   Back to cited text no. 32    
33.Durham LH, Willatt DJ, Yung MW, Jones I, Stevension PV, Ramadan MF. A method for preparation of fibrin glue. J Laryngol Otol 1987;101:1182-6.  Back to cited text no. 33    
34.DePalma L, Criss VR, Luban NL. The preparation of fibrinogen concentrate for use as fibrin glue by four different methods. Transfusion 1993;33:717-20.  Back to cited text no. 34    
35.Silver FH, Wang MC, Pins GD. Preparation of fibrin glue; a study of chemical and physical methods. J Appl Biomater 1995;6:175-83.  Back to cited text no. 35    
36.Cheng H, Fraidakis M, Biomback B, Lapchak P, Hoffer B, Olson L. Characterization of fibrin glue - GDNF slow - release preparation. Cell Transplant 1998;7:53-61.  Back to cited text no. 36    
37.Yoshida H, Hirozane K, Kamiya A. Adhesive strength of autologous fibrin glue. Biol Pharm Bull 2000;23:313-7.  Back to cited text no. 37    
38.Buchta C, Hedrich HC, Macher M, Hφcker P, Redl H. Biochemical characterization of autologous fibrin sealants produced by CryoSeal and Vivostat in comparison to the homologous fibrin sealant product Tissucol/Tisseel. Biomaterials 2005;26:6233-41.   Back to cited text no. 38    
39.Rock G, Neurath D, Lu M, Alharbi A, Freedman M. The contribution of platelets in the production of cryoprecipitates for use in a fibrin glue. Vox Sang 2006;91:252-5.  Back to cited text no. 39    
40.Rock G, Neurath D, Semple E, Harvey M, Freedman M. Preparation and characterization of human thrombin for use in a fibrin glue.Transfus Med 2007;17:187-91.  Back to cited text no. 40    
41.Evenson SA, Rollag H. Solvent/detergent-treated clotting factors and hepatitis A virus seroconversion. Lancet 1993;341:971-2.  Back to cited text no. 41    
42.Lefrere JJ, Mariotti M, Thauvin M. B19 parvovirus DNA in solvent/ detergent-treated anti-haemophilia concentrates. Lancet 1994;343:211-2.  Back to cited text no. 42    
43.Yoshida H, Kamiya A. A quicker preparation method for autologous fibrin glue. Biol Pharm Bull 1998:21:1367-70.  Back to cited text no. 43    
44.Gammon RR, Prum BE Jr, Avery N, Mintz PD. Rapid preparation of small volume autologous fibrinogen concentrate and its same day use in bleb leaks after glaucoma filtration surgery. Ophthalmic Surg Lasers 1998;29:1010-2.  Back to cited text no. 44    
45.Man D, Plosker H, Winland-Brown JE. The use of autologous platelet-rich plasma (platelet gel) and autologous platelet-poor plasma (fibrin glue) in cosmetic surgery. Plast Reconstr Surg 2001;107:229-37.  Back to cited text no. 45    
46.Yazawa M, Ogata H, Nakajima T, Mori T, Watanabe N, Handa M. Basic studies on the clinical applications of platelet-rich plasma. Cell Transplant 2003;12:509-18.  Back to cited text no. 46    
47.Everts PA, Knape JT, Weibrich G, Schonberger JP, Hoffman J, Overdevest EP, et al . Platelet-rich plasma and platelet gel; a review. J Extra Corpor Technol 2006;38:174-87.  Back to cited text no. 47    
48.Dohan DM, Choukroun J, Diss A, Dohan SL, Dohan AJ, Mouhyl J, et al . Platelet-rich fibribn (PRF); a second-generation platelet concentrate. Part I: Technological concepts and evolution. Oral Surg Oral Med Oral Pathol Oral Radiol Endod 2006;101:37-44.  Back to cited text no. 48    
49.Aizawa P, Winge S, Karlsson G. Large-scale preparation of thrombin from human plasma. Thromb Res 2008:122:560-7.  Back to cited text no. 49    
50.Alston SM, Solen KA, Sukavaneshvar S, Mohammad SF. In vivo efficacy of a new autologus fibrin sealant. J Surg Res 2008;146:143-8.  Back to cited text no. 50    
51.De Somer F, Delanghe J, Somers P, Debrouwere M, Van Nooten G. Mechanical and chemical characteristics of an autologus glue. J Biomed Mater Res A 2007;86:1106-12.  Back to cited text no. 51    
52.Valbonesi M. Fibrin glues of human origin. Best Pract Res Clin Haematol 2006;19:191-203.  Back to cited text no. 52    
53.Marone P, Monzillo V, Segu C, Antoniazzi E. Antibiotic-impregnated fibrin glue in ocular surgery: In vitro antibacterial activity. Ophthalmologica 1999;213:12-5.  Back to cited text no. 53    
54.Berguer R, Staerkel RL, Moore EE, Moore FA, Galloway WB, Mockus MB. Use of fibrin glue in deep hepatic wounds. J Trauma 1991;31:408-11.  Back to cited text no. 54    
55.Shirari T, Shimota H, Chida K, Sano S, Takeuchi Y, Yasueda H. Anaphylaxis to aprotinin in fibrin sealant. Intern Med 2005;44:1088-9.  Back to cited text no. 55    
56.Beieriein W, Scheule AM, Antoniadis G, Braun C, Schosser R. Anaphylaxis Transfusion 2000;40:302-5.  Back to cited text no. 56    
57.Jabs AD Jr, Wider TM, DeBellis J, Hugo NE, The effect of skin grafts in infected sites. Plastic Reconstruction Surg 1992;89:268-71.  Back to cited text no. 57    
58.Vedung S, Hedlung A. Fibrin glue Its use for skin grafting of contaminated burn wounds in areas difficult to mmobilize. J Burn Care Rehabil 1993;14:356-8.  Back to cited text no. 58    
59.Biswas NR, Das H., Satpathy G. Mohanty SS, Panda A. Role of aprotinin in the management of experimental fungalkeratitis. Ophthalmic Res 2001;33:147-50.  Back to cited text no. 59    
60.Chen WL, Lin CT,Hsieh CY, Tu IH, Chen WY, Hu FR. Comparison of the bacteriostatic effects, corneal cytotoxicity, and the ability to seal corneal incisions among three different tissue adhesives. Cornea 2007;26:12228-34.  Back to cited text no. 60    
61.Saltz R, Sierra D, Feldman D, Saltz MB, Dimick A, Vasconez LO. Experimental and clinical application of fibrin glue. Plast Reconstr Surg 1991;88:1005-15.   Back to cited text no. 61    
62.Bhatia SS. Ocular surface sealants and adhesives. Ocul Surf 2006;4:146-54.  Back to cited text no. 62    
63.Stenikogler FJ. Haddad R. Experimental experience with fibrin glued heterogenic pericardium in conjunctival surgery. In: Schlag G, Redl H, editors. Fibrin Sealant in Operative Medicine. Vol. 2. Ophthalmology-Neurosurgery. Berlin: Springer; 1986. p. 88- 91.   Back to cited text no. 63    
64.Zauberman H, Hemo I, Use of Fibrin glue in ocular surgery. Ophthalmic Surg 1988;19:132-3.  Back to cited text no. 64    
65.Cohen RA, McDonald MB. Fixation of conjunctival autografts with an organic tissue adhesive. Arch Ophthalmol 1993;111:1167-8.  Back to cited text no. 65    
66.Koranyi G, Seregard S, Kopp ED. Cut and paste: a no suture, small incision approach to pterygium surgery. Br J Ophthalmol 2004;88:911-4.  Back to cited text no. 66    
67.Koranyi G, Seregard S, Kopp ED. The cut-and-paste method for primary pterygium surgery: long-term follow-up. Acta Ophthalmol Scand 2005;83:298-301.  Back to cited text no. 67    
68.Kheirkhah A, Casas V, Blanco G, Li W, Hayashida Y, Chen YT, Tseng SC. Amniotic membrane transplantation with fibrin glue for conjunctivochalasis. Am J Ophthalmol 2007;144:311-3.  Back to cited text no. 68    
69.Uy HS, Reyes JM, Flores JD, Lim-Bon-Siong R. Comparison of fibrin glue and sutures for attaching conjunctival autografts after pterygium excision. Ophthalmology 2005;112:667-71.  Back to cited text no. 69    
70.Bahar I, Weinberger D, Dan G, Avisar R. Pterygium Surgery. Cornea 2006;25:1168-72.   Back to cited text no. 70    
71.Marticorena J, Rodrνguez-Ares MT, Touriρo R, Mera P, Valladares MJ, Martinez-de-la-Casa JM, et al . Pterygium surgery: Conjunctival autograft using a fibrin adhesive. Cornea 2006;25:34-6.  Back to cited text no. 71    
72.Bahar I, Weinberger D, Gaton DD, Avisar R. Fibrin glue versus vicryl sutures for primary conjunctival closure in pterygium surgery: long-term results. Curr Eye Res 2007;32:399-405.  Back to cited text no. 72    
73.JiangJ, Yang Y, Zhang M, Fu X, Bao X, Yao K. Comparison of fibrin sealant and sutures for conjunctival autograft fixation in pterygium surgery;one year followup. Ophthalmologica 2008;222:105-11.  Back to cited text no. 73    
74.Jain AK, Bansal R, Sukhija J. Human amniotic membrane transplantation with fibrin glue in management of primary pterygia; a new tuck-in technique. Cornea 2008;27:94-9.  Back to cited text no. 74    
75.Kheirkhah A, Gasas V, Sheha H, Raju VK, Tseng SC. Role of conjunctival inflammation in surgical outcome after amniotic membrane transplantation with or without fibrin glue for pterygium. Cornea 2008;27:56-63.  Back to cited text no. 75    
76.Srinivasan S, Slomovie AR.Eye rubbing conjunctival graft dehiscence following pterygium surgery with fibrin glue. Eye 2007;21:865-7.  Back to cited text no. 76    
77.Spierer A, Barequet I, Rosner M, Solomon AS, Martinowitz U. Reattachment of extraocular muscles using fibrin glue in a rabbit model. Invest Ophthalmol Vis Sci 1997;38:543-6.  Back to cited text no. 77    
78.Erbil H, Sinav S, Sullu Y, Kandemir B. An experimental study on the use of fibrin sealants in strabismus surgery. Turk J Pediatr 1991;33:111-6.  Back to cited text no. 78    
79.Biedner B, Rosenthal G. Conjunctival closure in strabismus surgery; Vicryl versus fibrin glue. Ophthalmic Surg Lasers 1996;27:967-70.  Back to cited text no. 79    
80.Dadeya S, Ms K. Strabismus surgery: Fibrin glue versus vicryl for conjunctival closure. Acta Ophthalmol Scand 2001;79:515-7.  Back to cited text no. 80    
81.Mohan K, Malhi RK, Sharma A, Kumar S. Fibrin glue for conjunctival closure in strabismus surgery. J Paediatric Ophthalmol Strabismus 2003;40:158-60.  Back to cited text no. 81    
82.Erbaqci I, Bekir N. Sutureless closure of the conjunctiva with a commercial fibrin sealant in strabismus. Strabismus 2007;15:89-4.  Back to cited text no. 82    
83.Tonelli E Jr, de Almeida HC, Bambirra EA. Tissue adhesives for a sutureless faden operation: An experimental study in a rabbit model. Invest Ophthalmol Vis Sci 2004;45:4340-5.  Back to cited text no. 83    
84.Lagoutte FM, Gauther L, Comte PRM. A fibrin sealant for perforated and preperforated corneal ulcers. Br J Ophthalmol 1989;73:757-61.   Back to cited text no. 84    
85.Vrabec MP, Jordan JJ. A surgical technique for the treatment of central corneal perforations J Ref Corneal Surg 1994;10:365-7.  Back to cited text no. 85    
86.Hick S, Demers PE, Brunette I, La C, Mabon M, Duchesne B. Amniotic membrane transplantation and fibrin glue in the management of corneal ulcers and perforations: A review of 33 cases Cornea 2005;24:369-77.  Back to cited text no. 86    
87.Bernauer W, Ficker LA, Watson PG, Dart JK. The management of corneal perforation associated with rheumatoid arthritis. An analysis of 32 eyes. Ophthalmology 1995;102:1325-37.  Back to cited text no. 87    
88.Duchesne B, Tahi H, Galand A. Use of human fibrin glue and amniotic membrane transplant in corneal perforation. Cornea 2001;20:230-2.  Back to cited text no. 88    
89.Sii F, Lee GA Fibrin glue in the management of corneal melt. Clin Experiment Ophthalmol 2005;33;532-4.  Back to cited text no. 89    
90.Sii F, Lee GA, Gole GA. Perforated corneal hydrops treated with sulfur hexafluoride (SF6) gas and tissue adhesive. Cornea 2005;24:503-4.  Back to cited text no. 90    
91.Liu BQ, Wang Zc, Liu LM, Liu JB, Li Ny Wang LN, Ma P, Jiang RZ, Ge J. Sutureless fixation of amniotic membrane patch as a therapeutic contact lens by using a polymethyl methacrylate ring and fibrin sealant in a rabbit model. Cornea 2008;27:74-9.  Back to cited text no. 91    
92.Kheirkhah (LCT)A, Casas V, Raju VK, Tseng SC. Sutureless amniotic membrane transplantation for partial limbal stem cell deficiency. Am J Ophthalmol 2008;145:787-94.  Back to cited text no. 92    
93.Sekiyama E, Nakamura T, Kurihara E, Cooper LJ, Fullwood NJ, Takaoka M, et al . Novel sutureless transplantation of bioadhesive-coated, freeze-dried amniotic membrane for ocular surface reconstruction. Invest Ophthalmol Vis Sci 2007;48:1528-34.  Back to cited text no. 93    
94.Rosenthal AR, Harbury C, Ebgen PR. Use of a platelet-fibrinogen-thrombin mixture as a corneal adhesive experiments with sutureless lamellar keratoplasty in the rabbit. Invest Ophthalmol Vis Sci 1975;14:872-5.  Back to cited text no. 94    
95.Kim MS, Kim JH. Effects of tissue adhesive (Tisseel) on corneal wound healing in lamellar keratoplasty in rabbits. Korean J Ophthalmol 1989;3:14-21.  Back to cited text no. 95    
96.Ibrahim-Elzembely HA, Kaufman SC, Kaufman HE. Human fibrin tissue glue for corneal lamellar adhesion in rabbits: A preliminary study. Cornea 2003;22:735-9.  Back to cited text no. 96    
97.Kaufman HE, Insler MS, Ibrahim-Elzembely HA, Kaufman SC. Human fibrin tissue adhesive for sutureless lamellar keratoplasty and scleral patch adhesion; a pilot study. Ophthalmology 2003;110:2168-72.  Back to cited text no. 97    
98.Duarte MC, Kim T. Sutureless lamellar keratoplasty: A modified approach for fibrin glue application. Cornea 2007;26:1127-8.  Back to cited text no. 98    
99.Narendran N, Mohamed S, Shah S. No sutures corneal grafting--a novel use of overlay sutures and fibrin glue in Deep Anterior Lamellar Keratoplasty. Cont Lens Anterior Eye 2007;30:207-9.  Back to cited text no. 99    
100.Ignacio TS, Nguyen TB, Chuck RS, Kurtz RM, Sarayba MA. Top hat wound configuration for penetrating keratoplasty using the femtosecond laser: a laboratory model. Cornea 2006;25;336-40.  Back to cited text no. 100    
101.Bahar I, Kaiserman I, Slomovic A, McAllunm P, David Rootman. Fibrin Glue for opposing wound edges in "Top Hat" Penetrating Keratoplasty-A laboratory study. Cornea 2007;26:1235-8.  Back to cited text no. 101    
102.Pfister RR, Sommers CL. Fibrin sealant in corneal stem cell transplantation. Cornea 2005;24:593-8.  Back to cited text no. 102    
103.Rostron CK, Brittain GP, Morton DB, Rees JE. Experimental epikeratophakia with biological adhesive. Arch Ophthalmol 1988;106:1103-6.  Back to cited text no. 103    
104.Brittain GP, Rostron CK, Morton DB, Rees JE. The use of a biological adhesive to achieve sutureless epikeratophakia. Eye 1989;3:56-63.  Back to cited text no. 104    
105.Uhlig CE, Gerding H. Fibrin sealing improves stability of corneal prostheses during vitreoretinal procedures. Retina 2003;23:209-14.  Back to cited text no. 105    
106.Anderson NJ, Hardten DR. Fibrin glue for the prevention of epithelial ingrowth after laser in situ keratomileusis. J Cataract Refract Surg 2003;29:1425-9.  Back to cited text no. 106    
107.Yeh DL, Bushley DM, Kim T. Treatment of traumatic LASIK flap dislocation and epithelial ingrowth with fibrin glue. Am J Ophthalmol 2006;141:960-2.  Back to cited text no. 107    
108.Narvaez J, Chakrabarty A, Chang K. Treatment of epithelial ingrowth after LASIK enhancement with a combined technique of mechanical debridement, flap suturing, and fibrin glue application. Cornea 2006;25:1115-7.  Back to cited text no. 108    
109.Bonatti JA, Bechara SJ, DalT Col MW, Cresta FB, Carricondo PC, Kara-Jose N. A fibrin-related line of research and theoretical possibilities for the use of fibrin glue as a temporary basal membrane in non-perforated corneal ulcers and in photorefractive keratectomy (PRK) -operated corneas. Arq Bras Oftalmol 2007;70;884-9.  Back to cited text no. 109    
110.O'Sullivan F, Dalton R, Rostron LK. Fibrin glue; an alternative method of wound closure in glaucoma surgery. J Glaucoma 1996;5:367-70.  Back to cited text no. 110    
111.Grewing R, Mester U. Fibrin sealant in the management of complicated hypotony after trabeculectomy. Ophthalmic Surg Lasers 1997;28:124-7.  Back to cited text no. 111    
112.Wright MM, Brown EA, Maxwell K, Cameron JD, Walsh AW. Laser-cured fibrinogen glue to repair bleb leaks in rabbits. Arch Ophthalmol 1998;116:199-202.  Back to cited text no. 112    
113.Seligsohn A, Moster MR, Steinmann W, Fontanarosa J. Use of Tisseel fibrin sealant to manage bleb leaks and hypotony;case series. J Glaucoma 2004;13:227.  Back to cited text no. 113    
114.Valimaki J.Fibrin glue for preventing immediate postoperative hypotony following glaucoma drainage implant surgery. Acta Ophthalmol Scand 2006;84:372-4.  Back to cited text no. 114    
115.Buschmann W. Microsurgical treatment of lens capsule perforations-Part II: Clinical applications and results. Ophthalmic Surg 1987;18:276-82.  Back to cited text no. 115    
116.Henrick A, Gaster RN, Silverstone PJ. Organic tissue glue in closure of cataract incisions. J Cataract Refract Surg 1987;13:551-5.  Back to cited text no. 116    
117.Mestr U, Zuche M, Rauber M. Astigmatism after Phaco with PCIOL: Small incision technique with fibrin adhesive for wound closure. JCRS 1993;19:616-9.  Back to cited text no. 117    
118.Buschmann W. Progress in fibrin sealing of eye lens and conjunctiva. In: Schlag G, Ascher PW, Steinkogler F Stammberger H, editors. Fibrin Sealing in Surgical and Nonsurgical Fields: Vol. 5. Neurosurgery, Ophthalmic Surgery, ENT. Berlin: Springer-Verlag; 1994. p. 97-106.   Back to cited text no. 118    
119.Mester U, Zuche M, Rauber M. Astigmatism after phacoemulsification with posterior chamber lens implantation: small incision technique with fibrin adhesive for wound closure. J Cataract Refract Surg 1993;19:616-9.  Back to cited text no. 119    
120.Alio JL, Mulet E, Sakla HF, Gobbi F. Efficacy of synthetic and biological bioadhesives in scleral tunnel Phaco in eyes with high myopia. JCRS 1998;24:983-8.  Back to cited text no. 120    
121.Agarwal A, Kumar DA, Jacob S, Baid C, Agarwal A, Srinivasan S. Fibrin glue-assisted sutureless posterior chamber intraocular lens implantation in eyes with deficient posterior capsules. J Cataract Refract Surg 2008;34:1433-8.  Back to cited text no. 121    
122.Mentens R, Devogelaere T, Stalmans P.Comparing fibrin glue to sutures for conjunctival closure in pars plana vitrectomy. Bull Soc Belge Ophthalmol 2007;306:49-56.  Back to cited text no. 122    
123.Mentens R, Stalmans P.Comparison of fibrin glue and sutres for conjunctival closure in pars plana vitrectomy. Am J Ophthalmol 2007;144:128-31.  Back to cited text no. 123    
124.Batman C, Ozdamar Y, Aslan O, Sonmez K, Mutevelli S, Zilelioglu G. Tissue glue in sutureless vitreoretinal surgery for the treatment of wound leakage. Ophthalmic Surg Lasers Imaging 2008;39:100-6.  Back to cited text no. 124    
125.Blumenkranz MS, Ohana E, Shaikh S, Chang S, Coll G, Morse LS, et al . Adjuvant methods in macular hole surgery: intraoperative plasma-thrombin mixture and postoperative fluid-gas exchange. Ophthalmic Surg Lasers 2001;32:198-207.  Back to cited text no. 125    
126.Steinkogler FJ. The use of fibrin sealant in lid surgery In: Schlag G, Redl H, editors. Fibrin Sealant in Operative Medicine: Vol. 2. Ophthalmology-Neurosurgery. Berlin: Springer; 1986. p. 85-7.  Back to cited text no. 126    
127.Mandel MA. Closure of blepharoplasty incisions with autologous fibrin glue. Arch Ophthalmol 1990;108:842-4.  Back to cited text no. 127    
128.Steinkogler FJ. Fibrin tissue adhesive for the repair of lacerated canaliculi lacrimales. In: Schlag G, Redl H, editors. Fibrin Sealant in Operative Medicine: Vol. 2. Ophthalmology-Neurosurgery. Berlin: Springer; 1986. p. 92-4.   Back to cited text no. 128    
129.Steinkogler FJ, Moser E. Canaliculo-cystostomy using the fibrin glue technique Fortschr Ophthalmol 1989;86:76-7.  Back to cited text no. 129    
130.Gosain AK, Lyon VB. Plastic Surgery Educational Foundation DATA Committee. The current status of Tissue Glues: Part II. For adhesion of soft tissues. Plastic Reconstr Surg 2002;110:1581-5.  Back to cited text no. 130    
131.Steinkogler FJ, Kuchar A. Fibrin sealant in ophthalmic plastic and reconstructive surgery. In: Schlag G, Ascher PW, Steinkogler F Stammberger H, editors. Fibrin Sealing in Surgical and Nonsurgical Fields: Vol. 5. Neurosurgery, Ophthalmic Surgery, ENT. Berlin: Springer-Verlag; 1994. p. 87-96.  Back to cited text no. 131    


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Medical Journal Armed Forces India. 2013;
[Pubmed]
8 Management of haemostasis in surgery
Daniele Lodi,Tommaso Iannitti,Beniamino Palmieri
Blood Coagulation & Fibrinolysis. 2012; 23(6): 465
[Pubmed]
9 Safety and Efficacy of Fibrin Glue versus Vicryl Sutures in Recurrent Pterygium with Amniotic Membrane Grafting
Reda A. Mahdy, Mostafa M. Wagieh
Ophthalmic Research. 2012; 47(1): 23
[VIEW]
10 Current variations of glaucoma filtration surgery :
Sarwat Salim
Current Opinion in Ophthalmology. 2012; 23(2): 89
[VIEW]
11 Use of a fibrin adhesive for conjunctival closure in trabeculectomy
José M. Martinez-de-la-Casa, Omar Rayward, Federico Saenz-Frances, Carmen Mendez, Enrique Santos Bueso, Julián Garcia-Feijoo
Acta Ophthalmologica. 2012; : no
[VIEW]
12 Comparison of bursting pressure after scleral tunnel incision sealed with sutures or an adherent ocular bandage in human globes
Jun, E.J., Kim, J.H., Purcell, T.L., Schanzlin, D.J.
Journal of International Medical Research. 2012; 40(2): 756-760
[Pubmed]
13 Fibrin glue coating of the surgical surfaces may facilitate formation of a successful bleb in trabeculectomy surgery
Yasar Sakarya, Rabia Sakarya, Selcuk Kara, Tulay Soylu
Medical Hypotheses. 2011;
[VIEW]
14 Fibrin Glue–Assisted Sutureless Limbal Stem Cell Transplantation Surgery for the Treatment of Severe Ocular Chemical Injury :
Baris Sonmez, Ümit Beden
Cornea. 2011; 30(3): 296
[VIEW]
15 Sutureless amniotic membrane transplantation combined with narrow-strip conjunctival autograft for pterygium
Hande Taylan Sekeroglu, Elif Erdem, Nese Cetin Dogan, Meltem Yagmur, Reha Ersoz, Ahmet Dogan
International Ophthalmology. 2011;
[VIEW]
16 Cut and paste: A novel method of reattaching rectus muscles with cyanoacrylate during recessions in strabismus
Agrawal, S., Singh, V.
Indian Journal of Ophthalmology. 2011; 59(5): 408-409
[Pubmed]
17 Can we overcome the challenges of sutures in lamellar keratoplasty
Panda, A., Kumar, S.
Indian Journal of Ophthalmology. 2011; 59(4): 308-310
[Pubmed]
18 Tissue adhesives as an alternative for conjunctival closure in strabismus surgeries
Basmak, H., Gursoy, H., Cakmak, A.I., Niyaz, L., Yildirim, N., Sahin, A.
Strabismus. 2011; 19(2): 59-62
[Pubmed]
19 A light-activated method for repair of corneal surface defects
Ying Wang, Irene E. Kochevar, Robert W. Redmond, Min Yao
Lasers in Surgery and Medicine. 2011; 43(6): 481
[VIEW]
20 Sharps injuries in ophthalmic practice
A-J Ghauri, K N Amissah-Arthur, A Rashid, B Mushtaq, M Nessim, S Elsherbiny
Eye. 2011; 25(4): 443
[VIEW]
21 Cauterization for the Prevention of Leaking Sclerotomies After 23-Gauge Transconjunctival Pars Plana Vitrectomy : An easy way to obtain sclerotomy closure
Francesco Boscia, Gianluca Besozzi, Nicola Recchimurzo, Luigi Sborgia, Claudio Furino
Retina. 2011; 31(5): 988
[VIEW]
22 Complications of strabismus surgery :
John W Simon
Current Opinion in Ophthalmology. 2010; 21(5): 361
[VIEW]
23 Polyethylene Glycol Hydrogel Polymer Sealant for Closure of Sutureless Sclerotomies: A Histologic Study
Ajay Singh, Mojgan Hosseini, Seenu M. Hariprasad
American Journal of Ophthalmology. 2010; 150(3): 346
[VIEW]
24 Fibrin glue in ophthalmology
Kumar, N., Al Sabti, K.
Indian Journal of Ophthalmology. 2010; 58(2): 176
[Pubmed]
25 Hemostats, sealants, and adhesives II: Update as well as how and when to use the components of the surgical toolbox
Spotnitz, W.D., Burks, S.
Clinical and Applied Thrombosis/Hemostasis. 2010; 16(5): 497-514
[Pubmed]
26 Polyethylene glycol hydrogel polymer sealant for closure of sutureless sclerotomies: A histologic study
Singh, A., Hosseini, M., Hariprasad, S.M.
American Journal of Ophthalmology. 2010; 150(3): 346-351
[Pubmed]
27 Cut and paste: A novel method of re-attaching rectus muscles with cyanoacrylate during recessions in strabismus
Darakshan, A., Amitava, A.
Indian Journal of Ophthalmology. 2010; 58(5): 395-398
[Pubmed]



 

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© 2005 - Indian Journal of Ophthalmology
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Online since 1st April '05