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Year : 2010  |  Volume : 58  |  Issue : 6  |  Page : 549-550

Sabouraud dextrose agar for the diagnosis of fungal keratitis

Wiwanitkit House, Bangkhae, Bangkok - 10160, Thailand

Date of Web Publication16-Oct-2010

Correspondence Address:
Viroj Wiwanitkit
Wiwanitkit House, Bangkhae, Bangkok - 10160
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Source of Support: None, Conflict of Interest: None

DOI: 10.4103/0301-4738.71684

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How to cite this article:
Wiwanitkit V. Sabouraud dextrose agar for the diagnosis of fungal keratitis. Indian J Ophthalmol 2010;58:549-50

How to cite this URL:
Wiwanitkit V. Sabouraud dextrose agar for the diagnosis of fungal keratitis. Indian J Ophthalmol [serial online] 2010 [cited 2020 Jun 4];58:549-50. Available from: http://www.ijo.in/text.asp?2010/58/6/549/71684

Dear Editor,

I read the recent publication on diagnosis of fungal keratitis with great interest. [1] Das et al. concluded that "This study recommends use of blood agar or chocolate agar for diagnosis of both bacterial and fungal keratitis in situations with limited resources." [1] Omitting sabouraud dextrose agar (SDA) seems to be a challenge. In microbiology, SDA is used as a standard for fungus study. [2] The use of few samples in this study should be carefully considered. In addition, some fungi, especially for Histoplasma spp. cannot be successfully cultured via blood or chocolate agars. [3],[4]

  References Top

Das S, Sharma S, Kar S, Sahu SK, Samal B, Mallick A. Is inclusion of Sabouraud dextrose agar essential for the laboratory diagnosis of fungal keratitis?. Indian J Ophthalmol 2010;58:281-6.  Back to cited text no. 1
[PUBMED]  Medknow Journal  
Odds FC. Sabouraud('s) agar. J Med Vet Mycol 1991;29:355-9.  Back to cited text no. 2
Procop GW, Cockerill FR 3rd, Vetter EA, Harmsen WS, Hughes JG, Roberts GD. Performance of five agar media for recovery of fungi from isolator blood cultures. J Clin Microbiol 2000;38:3827-9.  Back to cited text no. 3
Roberts GD, Washington JA 2nd. Detection of fungi in blood cultures. J Clin Microbiol 1975;1:309-10.  Back to cited text no. 4

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1 Authorsę reply
Sharma, S., Das, S.
Indian Journal of Ophthalmology. 2010; 58(6): 550


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