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ORIGINAL ARTICLE
Year : 2012  |  Volume : 60  |  Issue : 2  |  Page : 115-118

Sphingosine-1 phosphate prevents ethanol-induced corneal epithelial apoptosis


1 EA4555 Génétique des troubles de la réfraction et des anomalies du dévelopement de l'oeil, CHU Purpan; Service d'Ophtalmologie, CHU Purpan; and Université Toulouse III Paul Sabatier, Toulouse, 31000, France
2 EA4555 Génétique des troubles de la réfraction et des anomalies du dévelopement de l'oeil, CHU Purpan; Université Toulouse III Paul Sabatier, Toulouse, 31000, France
3 Université Toulouse III Paul Sabatier; Service d'Anatomie Pathologique, CHU Rangueil; and Inserm, U858, Institut de Médecine Moléculaire de Rangueil, Toulouse, 31432, France
4 Université Toulouse III Paul Sabatier; Laboratoire des IMRCP, UMR CNRS 5623, Toulouse, 31000, France

Correspondence Address:
Jean-Claude Quintyn
Service d'Ophtalmologie, CHU Purpan, Place du Dr Baylac, 31000 Toulouse Cedex 9
France
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Source of Support: None, Conflict of Interest: None


DOI: 10.4103/0301-4738.94052

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Background: Apoptosis is a programmed cell death in multicellular organisms, found in a wide variety of conditions, including inflammatory process, everywhere in the body, including the cornea and conjunctiva. Aim: To evaluate the effect of a new topical formulation of sphingosine-1 phosphate on preventing apoptosis of the corneal epithelium. Setting: Medical University. Materials and Methods: We tested several formulations suitable for topical application. Twenty-five rabbits were distributed among five groups. Group 1 comprised the controls. In Group 2, 20% ethanol was applied topically for 20 seconds; in Group 3, 50 μM topical sphingosine-1 phosphate was applied 2 hours prior to 20% ethanol application. In Group 4, 200 μM topical sphingosine-1 phosphate was applied 2 hours before the 20% ethanol application. In Group 5, only 200 μM topical sphingosine-1 phosphate was applied. Apoptosis was evaluated using the terminal deoxynucleotidyl transferase biotin-dUTP Nick End Labeling (TUNEL) assay. Pairwise comparisons were performed using t-tests with Scheffe's correction. Data were analyzed using STATA 9.0 statistical software. Results: A suspension of sphingosine-1 phosphate in the presence of Montanox 80 was stable and could be formulated without sonication. Epithelial apoptosis was detected only in Groups 2 and 3. Conclusion: Sphingosine-1 phosphate can prevent ethanol-induced apoptosis in the corneal epithelium of rabbits.


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