Glyxambi
Home About us Editorial board Ahead of print Current issue Search Archives Submit article Instructions Subscribe Contacts Login 
  • Users Online: 524
  • Home
  • Print this page
  • Email this page


 
   Table of Contents      
LETTER TO THE EDITOR
Year : 2019  |  Volume : 67  |  Issue : 2  |  Page : 307-308

Commentary: Pseudopestalotiopsis theae keratitis


Tej Kohli Cornea Institute, L. V. Prasad Eye Institute, Hyderabad, Telangana, India

Date of Web Publication23-Jan-2019

Correspondence Address:
Dr. Prashant Garg
L. V. Prasad Eye Institute, L. V. Prasad Marg, Banjara Hills, Hyderabad - 500 034, Telangana
India
Login to access the Email id

Source of Support: None, Conflict of Interest: None


DOI: 10.4103/ijo.IJO_1490_18

Rights and Permissions

How to cite this article:
Garg P. Commentary: Pseudopestalotiopsis theae keratitis. Indian J Ophthalmol 2019;67:307-8

How to cite this URL:
Garg P. Commentary: Pseudopestalotiopsis theae keratitis. Indian J Ophthalmol [serial online] 2019 [cited 2019 Apr 25];67:307-8. Available from: http://www.ijo.in/text.asp?2019/67/2/307/250665



Fungi are important causes of keratitis, especially in countries with a tropical climate. The organism group accounts for nearly 40% of all isolates from corneal ulcer cases.[1],[2] In the last decade, we have witnessed a lot of interest in the study of infections caused by fungi. The outbreak of Fusarium keratitis in contact lens users probably provided stimulus in generating this interest and necessary financial support. One of the fields that got attention of researchers is improvement in the identification of fungal isolates.

Classically, most laboratories use culture characteristics and morphology of spores for the identification of fungal isolates. Unfortunately, samples positive for fungus on microscopic examination might fail to grow on culture. Further, colonies on culture media might fail to sporulate. Both these factors result in a significant proportion of fungi remaining unidentified or isolates identified to the genus level only.

It is increasingly being recognized that accurate diagnosis of fungi to species level will throw new light in understanding the epidemiology, drug susceptibility, and treatment outcomes of mycotic keratitis cases. The most commonly used technique for species identification is sequence-based molecular identification, wherein a fragment from a specific locus is amplified by polymerase chain reaction (PCR), followed by the analysis of the resulting sequence with a nucleotide–nucleotide BLAST search in the nucleotide sequence collection of the National Center for Biotechnology Information (NCBI). The locus most frequently applied for species identification is the internal transcribed spacer (ITS) region of the ribosomal RNA gene cluster (rDNA). The application of this approach has resulted in the identification of several uncommon fungi from keratitis cases. Prominent among these isolates are Lagenidium and Pythium species from the Oomycota division.[3]

Using these techniques, an Indo–Hungarian Fungal Keratitis (IHFK) Working Group demonstrated an impressive phylogenetic diversity of the genus Fusarium causing human keratomycosis in South India.[4] The diversity was reflected in antimicrobial susceptibility as well. The group highlighted the need for species-level identification of fungal isolates both for understanding precise epidemiology as well as appropriate drug treatment.

In this issue of the journal, you will find an article [5] where authors described a case of keratitis caused by a plant pathogen Pseudopestalotiopsis theae. The organism belongs to Pestalotiopsis group that contains two genera Neopestalotiopsis and Pseudopestalotiopsis.

This is probably the first report of human infection caused by the organism and the precise identification was possible because of the use of molecular technique. The casereiterates the importance of definitive diagnosis of fungal isolates to species level.

To conclude, because in India fungi play an important role in the causation of keratitis, a potentially blinding disorder, it is time that few well-established laboratories must start adopting techniques that help identifying fungal isolates to species levels on a routine basis. This combined with antimicrobial susceptibility will help better define the epidemiology, medical management, and help understand the causes of treatment failure besides identifying uncommon pathogens.



 
  References Top

1.
Shah A, Sachdev A, Coggon D, Hossain P. Geographic variations in microbial keratitis: An analysis of the peer-reviewed literature. Br J Ophthalmol 2011;95:762-7.  Back to cited text no. 1
    
2.
Kredics L, Narendran V, Shobana C, Group I-H. Filamentous fungal infections of the cornea: A global overview of epidemiology and drug sensitivity. Mycoses 2015;58:243-60.  Back to cited text no. 2
    
3.
Sharma S, Balne PK, Motukupally SR, Das S, Garg P, Sahu SK, et al. Pythium insidiosum keratitis: Clinical profile and role of DNA sequencing and zoospore formation in diagnosis. Cornea 2015;34:438-42.  Back to cited text no. 3
    
4.
Hassan AS, Al-Hatmi AM, Shobana CS, Group I-H. Antifungal susceptibility and phylogeny of opportunistic members of the genus Fusarium causing human keratomycosis in South India. Medical Mycology 2016;54:287-94.  Back to cited text no. 4
    
5.
Sane S, Sharma S, Konduri R, Fernandes M. Emerging corneal pathogens:First report of Pseudopestalotiopsis theae keratitis. Indian J Ophthalmol 2019;67:150-2.  Back to cited text no. 5
[PUBMED]  [Full text]  




 

Top
 
 
  Search
 
    Similar in PUBMED
   Search Pubmed for
   Search in Google Scholar for
    Access Statistics
    Email Alert *
    Add to My List *
* Registration required (free)  

 
  In this article
References

 Article Access Statistics
    Viewed277    
    Printed1    
    Emailed0    
    PDF Downloaded40    
    Comments [Add]    

Recommend this journal