Home About us Editorial board Ahead of print Current issue Search Archives Submit article Instructions Subscribe Contacts Login 
  • Users Online: 3006
  • Home
  • Print this page
  • Email this page

   Table of Contents      
Year : 2020  |  Volume : 68  |  Issue : 7  |  Page : 1443-1444

Multi-drug resistant Burkholderia cepacia keratitis following laser in situ keratomileusis complicated by interface fluid syndrome

1 Cornea and Anterior Segment, LV Prasad Eye Institute, GMR Varalakshmi Campus, Visakhapatnam, Andhra Pradesh, India
2 Ocular Microbiology Service, LV Prasad Eye Institute, GMR Varalakshmi Campus, Visakhapatnam, Andhra Pradesh, India

Date of Submission05-Sep-2019
Date of Acceptance20-Feb-2020
Date of Web Publication25-Jun-2020

Correspondence Address:
Dr. Merle Fernandes
Cornea and Anterior Segment Service, L V Prasad Eye Institute, GMR Varalakshmi Campus, Hanumanthawaka Jn, Visakhapatnam - 530 040, Andhra Pradesh
Login to access the Email id

Source of Support: None, Conflict of Interest: None

DOI: 10.4103/ijo.IJO_1633_19

Rights and Permissions

Keywords: Burkholderia cepacia, interface fluid syndrome, post LASIK microbial keratitis

How to cite this article:
Kanduri V, Madduri B, Mohan N, Fernandes M. Multi-drug resistant Burkholderia cepacia keratitis following laser in situ keratomileusis complicated by interface fluid syndrome. Indian J Ophthalmol 2020;68:1443-4

How to cite this URL:
Kanduri V, Madduri B, Mohan N, Fernandes M. Multi-drug resistant Burkholderia cepacia keratitis following laser in situ keratomileusis complicated by interface fluid syndrome. Indian J Ophthalmol [serial online] 2020 [cited 2020 Jul 13];68:1443-4. Available from: http://www.ijo.in/text.asp?2020/68/7/1443/287505

A 26-year-old female was referred to us with left eye microbial keratitis following laser in-situ keratomileusis (LASIK) done a month ago. The vision was 20/1200. The left eye had a central infiltrate involving the flap and beyond [Figure 1]. Corneal scrapings revealed short gram-negative bacilli. Hourly topical fortified cefuroxime sodium (5%) and ofloxacin (0.3%) were commenced. Burkholderia cepacia cultures were sensitive to imipenem and amikacin, intermediate sensitivity to ceftazidime, and resistant to all other antibiotics using the VITEK 2 system (bioMerieux, USA). Therapy was switched to fortified amikacin sulfate (2.5%) eye drops, ceftazidime sodium (5%) eye drops hourly, and betamethasone sodium phosphate (0.1%) eye drops 4 times daily. Two weeks later, her vision deteriorated with the appearance of interface fluid [Figure 2]. Digital tension was elevated. Interface fluid disappeared following steroid discontinuation. The complete resolution took 3 months [Figure 3] with a vision of 20/50.
Figure 1: Image of the left eye with sclerotic scatter, showing a central epithelial defect, underlying patchy infiltrate (2.8 × 2.5 mm) involving flap and interface, dense cellular reaction in the interface in the form of an incomplete ring with dense cellularity at the flap margins involving the flap as well as the interface

Click here to view
Figure 2: (a) (upper right corner): Slit lamp image of the left eye showing interface fluid causing separation of the flap superiorly and inferiorly (arrow) and patchy infiltrate involving the flap and interface with anterior stromal scarring and diffuse cellularity in the interface extending till the flap edge without increase in congestion or anterior chamber reaction. (b) Anterior segment OCT (DRI OCT Triton, Topcon, Tokyo, Japan) of the cornea showing an elevated flap with fluid in the interface, and interface haze

Click here to view
Figure 3: Image of the left eye with sclerotic scatter, 4 months later, showing a central scar, with interface haze

Click here to view

  Discussion Top

B. cepacia is a notorious multidrug resistant organism and a significant cause of morbidity and mortality.[1] Keratitis due to Burkholderia species is uncommon. The risk factors include corneal transplants,[2],[3] prior herpes simplex virus (HSV) keratitis,[4] orthokeratology lens wear [5], and cataract surgery.[6] Microbial keratitis post LASIK is about 0–1.5%.[7] Burkolderia pickettii was responsible for diffuse lamellar keratitis following LASIK.[8] However, B. cepacia keratitis following LASIK is extremely rare with only one case reported prior.[9] Our patient had a patchy central infiltrate with a dense cellular reaction in the interface similar to an immune ring, hence topical steroids were started. This led to interface fluid syndrome (IFS), which resolved on discontinuation of steroids. IFS can occur following a steroid response, toxic anterior segment syndrome, herpetic keratouveitis, or dysfunctional endothelium [10] but has not been reported with microbial keratitis. Fluid accumulates in the potential space in the interface. Topical steroids or intraocular inflammation could have precipitated a rise in intraocular pressure and IFS in our case.

B. cepacia in our patient was multidrug resistant similar to a previous report.[9] However, a relapse was noted in the latter 3 weeks after treatment initiation. Our patient recovered completely over 3 months with a combination of ceftazidime and amikacin.

Multidrug resistant B. cepacia keratitis following LASIK is extremely rare necessitating prompt microbiological work-up with combination drug therapy for resolution of infection. IFS may occur due to topical steroids or intraocular inflammation and hence concomitant inflammation requires judicious use of steroids.

Declaration of patient consent

The authors certify that they have obtained all appropriate patient consent forms. In the form the patient(s) has/have given his/her/their consent for his/her/their images and other clinical information to be reported in the journal. The patients understand that their names and initials will not be published and due efforts will be made to conceal their identity, but anonymity cannot be guaranteed.

Financial support and sponsorship

This work was supported by the Hyderabad Eye Research Foundation.

Conflicts of interest

There are no conflicts of interest.

  References Top

Gautam V, Singhal L, Ray P. Burkholderia cepacia complex: Beyond pseudomonas and acinetobacter. Indian J Med Microbiol 2011;29:4-12.  Back to cited text no. 1
[PUBMED]  [Full text]  
Ritterband D, Shah M, Cohen K, Lawrence J, Seedor J. Burkholderia gladioli keratitis associated with consecutive recurrent endophthalmitis. Cornea 2002;21:602-3.  Back to cited text no. 2
Chaurasia S, Muralidhar R, Das S, Sangwan VS, Reddy AK, Gopinathan U. Keratitis caused by Burkholderia cepacia. Br J Ophthalmol 2011;95:746-7.  Back to cited text no. 3
Matoba AY. Polymicrobial keratitis secondary to Burkholderia ambifaria, Enterococcus, and Staphylococcus aureus in a patient with herpetic stromal keratitis. Am J Ophthalmol 2003;136:748-9.  Back to cited text no. 4
Ying-Cheng L, Chao-Kung L, Ko-Hua C, Wen-Ming H. Daytime orthokeratology associated with infectious keratitis by multiple Gram-negative bacilli: Burkholderia cepacia, Pseudomonas putida, and Pseudomonas aeruginosa. Eye Contact Lens 2006;32:19-20.  Back to cited text no. 5
Ornek K, Ozdemir M, Ergin A. Burkholderia cepacia keratitis with endophthalmitis. J Med Microbiol 2009;58:1517-8.  Back to cited text no. 6
Chang MA, Jain S, Azar DT. Infections following laser in situ keratomileusis: An integration of the published literature. Surv Ophthalmol 2004;49:269-80.  Back to cited text no. 7
Holland SP, Mathias RG, Morck DW, Chiu J, Slade SG. Diffuse lamellar Keratitis related to endotoxins released from sterilizer reservoir biofilms. Ophthalmology 2000;107:1227-33.  Back to cited text no. 8
Reddy JC, Tibbetts MD, Hammersmith KM, Nagra PK, Rapuano CJ. Successful management of Burkholderia cepacia keratitis after LASIK. J Refract Surg 2013;29:8-9.  Back to cited text no. 9
Randleman JB, Shah RD. LASIK Interface complications: Etiology, risk factors and outcomes. J Refract Surg 2012;28:575-86.  Back to cited text no. 10


  [Figure 1], [Figure 2], [Figure 3]


    Similar in PUBMED
   Search Pubmed for
   Search in Google Scholar for
 Related articles
    Access Statistics
    Email Alert *
    Add to My List *
* Registration required (free)  

  In this article
Article Figures

 Article Access Statistics
    PDF Downloaded35    
    Comments [Add]    

Recommend this journal