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OPHTHALMIC IMAGE
Year : 2020  |  Volume : 68  |  Issue : 8  |  Page : 1665

Human corneo-conjunctival limbus anatomy assessed by scanning electron microscopy


Hospital Clinic of Barcelona, Barcelona, Spain

Date of Web Publication24-Jul-2020

Correspondence Address:
Dr. Carlos Rocha de Lossada
Sabino de Arana 1, Barcelona
Spain
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Source of Support: None, Conflict of Interest: None


DOI: 10.4103/ijo.IJO_2205_19

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How to cite this article:
Peraza Nieves J, Rocha de Lossada C, Sabater Cruz N, Torras Sanvicens J. Human corneo-conjunctival limbus anatomy assessed by scanning electron microscopy. Indian J Ophthalmol 2020;68:1665

How to cite this URL:
Peraza Nieves J, Rocha de Lossada C, Sabater Cruz N, Torras Sanvicens J. Human corneo-conjunctival limbus anatomy assessed by scanning electron microscopy. Indian J Ophthalmol [serial online] 2020 [cited 2020 Aug 10];68:1665. Available from: http://www.ijo.in/text.asp?2020/68/8/1665/290427



Limbus is formed by the junction of the corneal and conjunctival epithelia, where the epithelium gradually becomes thicker toward the sclera.[1] It is a 1–2-mm-wide anatomical ring-shaped transition zone between the opaque sclera and the clear cornea.[2] Anatomically, the limbus contains important features related to eye functions including fibrovascular ridges radially oriented known as palisades of Vogt that host corneal stem cells for epithelial turnover.[1] Scanning electron microscopy (SEM) is a technique for obtaining high-resolution images of biological and nonbiological specimens.

Our aim was to show the ultrastructure of the corneal limbus using SEM [Figure 1].[3]
Figure 1: (×150): Red arrow: conjunctiva; yellow arrows: limbal cryptx; green arrows: stromal projections; blue arrow: epithelial basement membrane. Superficial stromal projections around the limbal crypts providing a niche for stem and epithelial cells. There is a transitional zone form peripheral projections to a smoother epithelial basement membrane centrally

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Financial support and sponsorship

Nil.

Conflicts of interest

There are no conflicts of interest.



 
  References Top

1.
Consejo A, Llorens-Quintana C, Radhakrishnan H, Iskander DR. Mean shape of the human limbus. J Cataract Refract Surg 2017;43:667-72.  Back to cited text no. 1
    
2.
Spaniol K, Witt J, Mertsch S, Borrelli M, Geerling G, Schrader S. Generation and characterisation of decellularised human corneal limbus. Graefes Arch Clin Exp Ophthalmol 2018;256:547-57.  Back to cited text no. 2
    
3.
Versura P, Bonvicini F, Caramazza R, Laschi R. Scanning electron microscopy study of human cornea and conjunctiva in normal and various pathological conditions. Scan Electron Microsc 1985;:1695-708.  Back to cited text no. 3
    


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  [Figure 1]



 

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