Correspondence Address:
S Chakraborty

Full Text

We would like to thank Dr. Chakraborty, for the comments. As we have already mentioned in the discussion, trypan blue, is taken up only by dead or damaged endothelial cells, and the normal cells remain unstained. So theoretically using the dye without the air bubble should not be a cause for concern. But whenever any study is carried out, it must precisely follow the guidelines set by previous researchers. This is to demonstrate that the technique is reproducible under our conditions.

We too had tried using the dye in some cases without the air bubble, after our study was completed. We too did not find much difference. But in absence of larger studies without use of air, we are unable to comment further.

While injecting the air bubble, multiple air bubbles cause irregular staining of the capsule. But this irregular staining actually does not cause much difficulty during the CCC. Also, while injecting the dye, one should move the cannula on the surface of the capsule and not just inject it from one point. This will result in more even spreading of the dye over the capsule.