Indian Journal of Ophthalmology

ORIGINAL ARTICLE
Year
: 2008  |  Volume : 56  |  Issue : 3  |  Page : 197--201

Transconjunctival penetration of mitomycin C


T Velpandian, Ramanjit Sihota, Ankur Sinha, Viney Gupta 
 Dr. Rajendra Prasad Centre for Ophthalmic Sciences, All India Institute of Medical Sciences, New Delhi, India

Correspondence Address:
Ankur Sinha
Dr. Rajendra Prasad Centre for Ophthalmic Sciences, All India Institute of Medical Science, Ansari Nagar, New Delhi - 110 029
India

Aims: The study was performed to estimate transconjunctival penetration of mitomycin C (MMC) to Tenon�SQ�s tissue following application over the intact conjunctiva before routine trabeculectomy. Settings and Design: Institution-based case series. Materials and Methods: In 41 eyes of 41 patients, MMC (0.4 mg/ml for 3 min) was applied over the intact conjunctiva before beginning trabeculectomy. Tenon�SQ�s capsule directly beneath the site of application was excised during trabeculectomy and was homogenized, centrifuged and MMC concentrations were analyzed using high-performance liquid chromatography (HPLC). Statistical Analysis Used: Statistical analysis was performed using stata0 8.0 version software (STATA Corporation, Houston, TX, USA). In this study, P -values less than 0.05 were considered as statistically significant. Results: The average weight of the sample of Tenon�SQ�s tissue excised was 5.51 � 4.42 mg (range: 0.9-17.1) and the average estimated MMC concentration found to be present in Tenon�SQ�s tissue using HPLC was 18.67 � 32.36 � 10−6 moles/kg of the tissue (range: 0.38-197.05 � 10−6 ). In 36 of the 41 patients (87.80%), the MMC concentration reached above 2 � 10−6 moles/kg of the tissue concentration required to inhibit human conjunctival fibroblasts. Conclusions: Mitomycin C does permeate into the subconjunctival tissue after supraconjunctival application for 3 min. Application of MMC over the conjunctiva may be a useful alternative to subconjunctival or subscleral application during routine trabeculectomy and as an adjunct for failing blebs.


How to cite this article:
Velpandian T, Sihota R, Sinha A, Gupta V. Transconjunctival penetration of mitomycin C.Indian J Ophthalmol 2008;56:197-201


How to cite this URL:
Velpandian T, Sihota R, Sinha A, Gupta V. Transconjunctival penetration of mitomycin C. Indian J Ophthalmol [serial online] 2008 [cited 2024 Mar 29 ];56:197-201
Available from: https://journals.lww.com/ijo/pages/default.aspx/article.asp?issn=0301-4738;year=2008;volume=56;issue=3;spage=197;epage=201;aulast=Velpandian;type=0