Indian Journal of Ophthalmology

ORIGINAL ARTICLE - AIOS AWARD PAPER*
Year
: 2020  |  Volume : 68  |  Issue : 4  |  Page : 565--572

Evaluation of thrombospondin–1 gene polymorphisms in corneal allograft rejection in Asian Indian patients


Murugesan Vanathi1, Rashmi Shukla2, Prahlad Balakrishnan2, Roopa Dwivedi1, Noopur Gupta1, Radhika Tandon1 
1 Cornea, Ocular Surface, Cataract and Refractive Services, Dr R P Centre for Ophthalmic Sciences, New Delhi, India
2 Department of Pediatric Genetics, All India Institute of Medical Sciences, New Delhi, India

Correspondence Address:
Dr. Murugesan Vanathi
Cornea and Ocular Surface Services, Room No 475, IVth Floor, Dr Rajendra Prasad Centre for Ophthalmic Sciences, All India Institute of Medical Sciences, Ansari Nagar, New Delhi - 110 029
India

Purpose: To evaluate the frequency and the association of Thrombospondin 1 (THBS1) gene single nucleotide polymorphisms (SNPs) in Asian Indian patients with optical full thickness corneal grafting surgery. Methods: Prospective case–control analysis of optical penetrating keratoplasty patients with and without immune rejection and controls for genotyping of 3 THBS1 gene SNPs (rs1478604 A>G; rs2228261 C>T; rs2228262 A>G) by Amplification Refractory Mutation System-Polymerase Chain Reaction (ARMS PCR). Results: Among 58 patients [45 with immune allograft rejection (DNA isolation was possible in 38 samples) and 13 without immune corneal allograft rejection] and 65 controls, allele frequencies observed for rs1478604 (A>G) are A: 69.7% and 72.6%, G: 30.2% and 27.3%; for rs2228261 (C>T) are T: 70.2% and 62.3%, C: 29.7% and 37.6%; and for rs2228262 (A>G) A: 97.4% and 98.4%; G 2.5% and 1.5% respectively. Genotype frequencies were rs1478604 (A>G) AA: 57.8% and 59.3%, AG 23.6% and 26.5%; GG 18.4% and 14%; for rs2228261 (C>T) TT: 40.5% and 33.8%, TC: 59% and 56.9%, CC: 0% and 9.2%; for rs2228262 (A>G) AA: 94.8% and 96.8%, AG: 5.1% and 3.1% in rejection and controls respectively. The allele and genotype frequency for the 3 described THSB1 SNPs did not show any difference between the corneal graft immune rejection patients and controls. Conclusion: Asian Indian population evaluated for THBS1 gene SNPs by ARMS PCR genotyping in Asian Indian population did not show any genetic association to immune rejection occurrence in our study.


How to cite this article:
Vanathi M, Shukla R, Balakrishnan P, Dwivedi R, Gupta N, Tandon R. Evaluation of thrombospondin–1 gene polymorphisms in corneal allograft rejection in Asian Indian patients.Indian J Ophthalmol 2020;68:565-572


How to cite this URL:
Vanathi M, Shukla R, Balakrishnan P, Dwivedi R, Gupta N, Tandon R. Evaluation of thrombospondin–1 gene polymorphisms in corneal allograft rejection in Asian Indian patients. Indian J Ophthalmol [serial online] 2020 [cited 2024 Mar 29 ];68:565-572
Available from: https://journals.lww.com/ijo/pages/default.aspx/article.asp?issn=0301-4738;year=2020;volume=68;issue=4;spage=565;epage=572;aulast=Vanathi;type=0