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Figure 3: Agarose gel electrophoretogram showing results of ITS PCR applied to ocular specimens after two rounds of amplifi cation Lane 1: Negative control (II round) Lane 2: Negative control (I round) Lane 3: M 5137/05 AH negative Lane 4: M 865/ 03 VF Positive (556bp I round 255bp II round) Lane 5: M 4921/05 VF Positive (300bp II round) Lane 6: M 279/05 AH Positive (595bp I round, 328bp II round) Lane 7: 4890/05 AH negative Lane 8: 464/03 VF Positive (328bp II round) Lane 9 M 536/05 VF Positive (595bp I round, 282bp II round) Lane 10: 3612/05 VF: 320bp (II round) Lane 11: M 2542/05 VF Positive (595bp I round, 310bp II round). Lane 12:M 4642 /05 VF Positive (320bp II round) Lane 13: Positive control: Candida albicans ATCC 24433 MW: Molecular weight marker Phi X 174 bacteriophage DNA, Hinf I digest |
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