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  Indian J Med Microbiol
 

Figure 3: Quantitative measurement of type I/type III collagen fibers by ImageJ. (a) Type I collagen fibers are recognized by their thick fibers and bright red or yellow staining, type III collagen fibers are characterized by their slender fibers and green staining. (b) The researcher manually drew the borders of the stained iris tissue. The total area of demarcated iris tissue is measured. (c and d) Type I and type III collagen fibers are differentiated by “split channels” in ImageJ due to their different colors. The morphometry of the cumulative areas of type I/type III collagen are measured

Figure 3: Quantitative measurement of type I/type III collagen fibers by ImageJ. (a) Type I collagen fibers are recognized by their thick fibers and bright red or yellow staining, type III collagen fibers are characterized by their slender fibers and green staining. (b) The researcher manually drew the borders of the stained iris tissue. The total area of demarcated iris tissue is measured. (c and d) Type I and type III collagen fibers are differentiated by “split channels” in ImageJ due to their different colors. The morphometry of the cumulative areas of type I/type III collagen are measured