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  Indian J Med Microbiol
 

Figure 1: Expression of IL-27 p28 and EBI3 protein in the cornea of HSK mice following corneal HSV-1 infection. (a) Expression of IL-27 p28 and EBI3 protein in the corneas was assessed by western blot analysis. *P< 0.01 indicates differences between HSK mice and uninfected mice (n = 6 in each group). (b) The corneal cryostat sections were immunostained with goat anti-mice IL-27 p28 antibody and donkey anti-goat IgG (green), and/or with rabbit anti-mice EBI3 antibody and donkey anti-rabbit IgG (red). Nuclei were counterstained with DAPI (blue fluorescence). (1) Neither IL-27 p28 nor EBI3 protein was expressed in the cornea of uninfected mice, only nuclei are shown in blue, (2) Both IL-27 p28 and EBI3 subunit were expressed simultaneously in the corneal epithelium, stroma, and endothelium of HSK mice (yellow-green fluorescence), (3) IL-27 p28 protein was shown in green, and (4) EBI3 protein was shown in red. Original magnification, ×400

Figure 1: Expression of IL-27 p28 and EBI3 protein in the cornea of HSK mice following corneal HSV-1 infection. (a) Expression of IL-27 p28 and EBI3 protein in the corneas was assessed by western blot analysis. *<i>P</i>< 0.01 indicates differences between HSK mice and uninfected mice (<i>n</i> = 6 in each group). (b) The corneal cryostat sections were immunostained with goat anti-mice IL-27 p28 antibody and donkey anti-goat IgG (green), and/or with rabbit anti-mice EBI3 antibody and donkey anti-rabbit IgG (red). Nuclei were counterstained with DAPI (blue fluorescence). (1) Neither IL-27 p28 nor EBI3 protein was expressed in the cornea of uninfected mice, only nuclei are shown in blue, (2) Both IL-27 p28 and EBI3 subunit were expressed simultaneously in the corneal epithelium, stroma, and endothelium of HSK mice (yellow-green fluorescence), (3) IL-27 p28 protein was shown in green, and (4) EBI3 protein was shown in red. Original magnification, ×400