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  Indian J Med Microbiol
 

Figure 2: Anti-IL-27 treatment reduces the severity of HSK and CD4+ T cell infiltrations in cornea. BALB/c mice were infected with 105 PFU HSV-1 (KOS). BALB/c mice received an intraperitoneal injection of 100 μg anti-IL-27 antibodies or IgG isotype control on days -1, +1, +3, and +5 relative to the viral infection. HSK lesion severity was determined by slit-lamp biomicroscopy, and the clinical severity of stromal keratitis of individually scored mice was recorded. Stromal keratitis was graded from 0 to 4+, depending on the corneal opacity with neovascularization, edema, and infiltration. Each group of mice consisted of twelve animals, and the results shown are the representative of two similar experiments. (a) The average scores of twelve mice per group over a period of 14 days post infection. Data are recorded as mean±SEM. Anti-IL-27 treated group showed the decreased corneal lesion compared to IgG treated group, with significant differences occurring on days 8, 10, 12 and 14 (*P<0.01). (b) Each dot represents corneal opacity of an individual BALB/c eye on the 14th day following HSV-1 infection. Crossbar indicates the mean. *P<0.01 vs IgG treated group. (c) Pictures representative for naïve eye, IgG treated eye, and anti-IL-27 treated eye were taken at day 14 post infection. (d) The typical histological findings of cornea stained with hematoxylin and eosin by day 14 after infection were shown. The naïve mouse showed normal corneal tissue. The cornea of the IgG treated group exhibited severely swollen, heavily infiltrated with inflammatory cells, and numerous neovascular tissues in the stroma. The cornea of anti-IL-27 treated group showed decreased stromal swelling, few inflammatory cell infiltration, and less neovasculars in the stroma. Original magnification, 400× (e) Six corneal samples from each group of treated mice were liberase digested at day 14 postinfection. The bars represent the total number of viable cells present per cornea of two groups. *P<0.01 compared with IgG-treated group. (f) The cells isolated from infected corneas were stained for CD4 marker and the bars represent the total number of CD4+ T cells present per cornea from two groups of mice. Decreased number of CD4+ T cells in the cornea of anti-IL-27 treated mice (*P<0.01 compared with IgG-treated group). (g) The percentage of CD4+ T cells in total cells of per cornea from two groups. Reduced percentage of CD4+ T cells in the cornea of anti-IL-27 treated mice (*P<0.01 compared with IgG treated group). (h) Representative plot denotes the percentage of inflammatory cells expressing CD4+ T cells markers on cornea of different group mice

Figure 2: Anti-IL-27 treatment reduces the severity of HSK and CD4<sup>+</sup> T cell infiltrations in cornea. BALB/c mice were infected with 105 PFU HSV-1 (KOS). BALB/c mice received an intraperitoneal injection of 100 μg anti-IL-27 antibodies or IgG isotype control on days -1, +1, +3, and +5 relative to the viral infection. HSK lesion severity was determined by slit-lamp biomicroscopy, and the clinical severity of stromal keratitis of individually scored mice was recorded. Stromal keratitis was graded from 0 to 4+, depending on the corneal opacity with neovascularization, edema, and infiltration. Each group of mice consisted of twelve animals, and the results shown are the representative of two similar experiments. (a) The average scores of twelve mice per group over a period of 14 days post infection. Data are recorded as mean±SEM. Anti-IL-27 treated group showed the decreased corneal lesion compared to IgG treated group, with significant differences occurring on days 8, 10, 12 and 14 (*<i>P</i><0.01). (b) Each dot represents corneal opacity of an individual BALB/c eye on the 14th day following HSV-1 infection. Crossbar indicates the mean. *<i>P</i><0.01 vs IgG treated group. (c) Pictures representative for naïve eye, IgG treated eye, and anti-IL-27 treated eye were taken at day 14 post infection. (d) The typical histological findings of cornea stained with hematoxylin and eosin by day 14 after infection were shown. The naïve mouse showed normal corneal tissue. The cornea of the IgG treated group exhibited severely swollen, heavily infiltrated with inflammatory cells, and numerous neovascular tissues in the stroma. The cornea of anti-IL-27 treated group showed decreased stromal swelling, few inflammatory cell infiltration, and less neovasculars in the stroma. Original magnification, 400× (e) Six corneal samples from each group of treated mice were liberase digested at day 14 postinfection. The bars represent the total number of viable cells present per cornea of two groups. *<i>P</i><0.01 compared with IgG-treated group. (f) The cells isolated from infected corneas were stained for CD4 marker and the bars represent the total number of CD4<sup>+</sup> T cells present per cornea from two groups of mice. Decreased number of CD4<sup>+</sup> T cells in the cornea of anti-IL-27 treated mice (*<i>P</i><0.01 compared with IgG-treated group). (g) The percentage of CD4<sup>+</sup> T cells in total cells of per cornea from two groups. Reduced percentage of CD4<sup>+</sup> T cells in the cornea of anti-IL-27 treated mice (*<i>P</i><0.01 compared with IgG treated group). (h) Representative plot denotes the percentage of inflammatory cells expressing CD4<sup>+</sup> T cells markers on cornea of different group mice