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  Indian J Med Microbiol
 

Figure 3: (a) 10% KOH wet mount preparation of corneal scraping showed long sparsely septate hyaline hyphae of Pythium insidiosum. The presence of numerous vesicles within the hyphae is usually observed. (b) Gram stain image showed the thick cell wall, a few septate, and mass of vesicles inside. (c) A 3 day old culture of P. insidiosum at 37°C grown on 5% sheep blood agar. (d) A vesicles with zoospores that developed after 3 h incubation before zoospore release (×10)

Figure 3: (a) 10% KOH wet mount preparation of corneal scraping showed long sparsely septate hyaline hyphae of <i>Pythium insidiosum</i>. The presence of numerous vesicles within the hyphae is usually observed. (b) Gram stain image showed the thick cell wall, a few septate, and mass of vesicles inside. (c) A 3 day old culture of <i>P. insidiosum</i> at 37°C grown on 5% sheep blood agar. (d) A vesicles with zoospores that developed after 3 h incubation before zoospore release (×10)