Close
  Indian J Med Microbiol
 

Figure 3: (Reproduced from Reference No. 44) flat-mount retinas from control subjects (ctrl) compared with AD patients stained with anti-Aβ42C-terminal-specific antibodies (12F4) and visualized with peroxidase-based labelling (DAB). (a) Control. (b and c) Aβ plaques along a retinal blood vessel. (d and e) Aβ42detected by fluorescence labelling (yellow), using curcumin (cur) staining (green), 12F4 staining (red), and 42,6-diamidino-2-phenylindole (DAPI) nuclear staining (blue). Sudan Black B (SBB) suppresses nonspecific autofluorescence. (f) Extracellular Aβ and cytosolic Aβ40using Cur and anti-Aβ40C-terminal-specific antibodies (11A5-B10) staining. Arrows indicate Aβ plaques

Figure 3: (Reproduced from Reference No. 44) flat-mount retinas from control subjects (ctrl) compared with AD patients stained with anti-Aβ<sub>42</sub>C-terminal-specific antibodies (12F4) and visualized with peroxidase-based labelling (DAB). (a) Control. (b and c) Aβ plaques along a retinal blood vessel. (d and e) Aβ<sub>42</sub>detected by fluorescence labelling (<i>yellow</i>), using curcumin (cur) staining (<i>green</i>), 12F4 staining (<i>red</i>), and 4<sup>2</sup>,6-diamidino-2-phenylindole (DAPI) nuclear staining (<i>blue</i>). Sudan Black B (SBB) suppresses nonspecific autofluorescence. (f) Extracellular Aβ and cytosolic Aβ<sub>40</sub>using Cur and anti-Aβ<sub>40</sub>C-terminal-specific antibodies (11A5-B10) staining. <i>Arrows</i> indicate Aβ plaques