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Figure 2: The graph showing the amplification curve obtained from the real-time polymerase chain reaction (RT-PCR). The blue line corresponds to CMV whereas the orange one corresponds to VZV. An increase in fluorescence after multiple amplification cycles denotes the presence of the viral genes in the sample (a); The flat lines denote the control arm. 6-Carboxyfluorescein (FAM) dye was used to detect any fluorescent activity. The flat lines indicate the absence of significant fluorescent activity (b) |
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