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Year : 1979  |  Volume : 27  |  Issue : 4  |  Page : 28-29

Electrophoretic and immunological studies of aqueous and serum in cases of uveitis

Railway Hospital, Allahabad, India

Correspondence Address:
N K Bhatnagar
Eye Surgeon, Railway Hospital Allahabad
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Source of Support: None, Conflict of Interest: None

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How to cite this article:
Bhatnagar N K, Singh M. Electrophoretic and immunological studies of aqueous and serum in cases of uveitis. Indian J Ophthalmol 1979;27:28-9

How to cite this URL:
Bhatnagar N K, Singh M. Electrophoretic and immunological studies of aqueous and serum in cases of uveitis. Indian J Ophthalmol [serial online] 1979 [cited 2021 Apr 18];27:28-9. Available from: https://www.ijo.in/text.asp?1979/27/4/28/32565

Table 1

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Table 1

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The protein content of the aqueous rises significantly in uveal inflammation, mostly due to increased permeability of anterior uveal capillaries. There are some evidences to suggest that immunoglobulines may be getting manu­factured in the uveal tissue as a local immulo­gic response to a variety of antigen including lens proteins[2]. Besides, ocular inflammation does influence the globulin factors in the circu­lating serum. Thus, a qualitative analysis of protein fraction of aqueous and serum is expec­ted to be valuable in the understanding of pathophysiology of uveitis.

With this background the present work was undertaken.

  Material and methods Top

All cases were adults. Following groups of cases were taken for study:­

(1) Traumatic iritis with macroscopic and micro­scopic hyphaema (10).

(2) Post traumatic lens induced uveitis with escape of lens matter in anterior chamber. In this group hyper­mature cataract with lens induced uveitis and secondary glaucoma were also included (15).

(3) Uveitis of non-specific nature; both recurrent and with hypopyon (5).

(4) Control group of senile cataract cases (20).

In each case 2 specimens were:

1. Aqueous was withdrawn by an ordinary syringe and needle.

2. 5 ml. blood from same patients was collected for the serological tests.


(i) Sera and aqueous were subjected to electro­phoresis.

(ii) The sera of patients were tested for compli­ment fixing antibodies against lens proteins in cases of traumatic uveitis and also against non specific antigen in cases of other uveitis.


No significant difference was seen between age group and sex hence these factors were eliminated from report.

The results of CFT and RBC agglutination ran almost parallel to each other. At times CET appeared simpler, more sensitive as well as more specific for antibodies.

  Conclusions Top

1. An effort has been made to recognise and isolate presence of immune responsible gamma globulins in cases of uveitis than simple mention of proteins as a result of inflamma­tion.

2. Electrophoretic pattern of aqueous protein is slightly different in cases of non­granulomatous as against lens induced granu­lomatous response. There being no protein in normal subject, the bands are absent.

3. Presence of gamma globulins in aqueous in all types of uveitis cases is indicative of local immunological response of uveal tissue to different antigen (T cell immune response) and there being no effect in titre of compliment fix­ing antibody of serum, as, response is not humoral, except that of lens induced variety where compliment fixing antibodies are present in all cases.

4. Presence of compliment fixing antibodies in serum in cases of lens induced uveitis seems to be a humoral response and chain of B-cell mediated response is affected producing circu­lating antibodies. This explains the hypersensi­tivity of uveal tissue in non-injured eye in case of sympathetic ophthalmitis as reported by other workers.

5. Electrophoretic bands of aqueous in traumatic uveitis with hyphaema and serum were similar probably because of presence of blood in aqueous.

  Summary Top

A simple technique of recognising gamma globulin has been described. Work for specific varieties of antibodies and positive control is still in progress.

  References Top

Witmer, R., 1975, Phacogene Uveitis Ophthal­mologica, Basel, 133, 326.  Back to cited text no. 1
Miller, H., 1963, Trans. Ophthal. Soc. U.K., 83, 689.  Back to cited text no. 2


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