Home About us Editorial board Ahead of print Current issue Search Archives Submit article Instructions Subscribe Contacts Login 
  • Users Online: 2028
  • Home
  • Print this page
  • Email this page

   Table of Contents      
ARTICLES
Year : 1981  |  Volume : 29  |  Issue : 3  |  Page : 151-152

Lens organ culture


Dr. V.M. Medical College, Solapur, India

Correspondence Address:
A G Chandorkar
Department of Pharmacology, Dr. V.M Medical College, Solapur-413 003
India
Login to access the Email id

Source of Support: None, Conflict of Interest: None


PMID: 7346418

Rights and PermissionsRights and Permissions

How to cite this article:
Chandorkar A G, Albal M V, Bulakh P M, Muley M P. Lens organ culture. Indian J Ophthalmol 1981;29:151-2

How to cite this URL:
Chandorkar A G, Albal M V, Bulakh P M, Muley M P. Lens organ culture. Indian J Ophthalmol [serial online] 1981 [cited 2020 Nov 30];29:151-2. Available from: https://www.ijo.in/text.asp?1981/29/3/151/30868

The incubation of the total lens either in a continuous perfusion fluid or immersed in tissue culture media constitutes a form of organ culture. Lens culture studies, in which the lens was maintained viable for 1 to 3 days, have been reported using either direct lens immersion in tissue culture media,[1],[2],[3] or in a perfusion system,[4],[5]. Almost all reported me­thods use lenses of rabbits. Hence, the present study was undertaken to evaluate the possi­bility of using lenses from human, goats, rats and rabbits for organ culture and to study the effect of varying, pH, temperature, media, anatomical positions and submersion or ex­posure to air.


  Materials and methods Top


Lenses obtained from the human patients, goats, rabbits and rats were used for the study. Human lenses collected from operation theatre, were kept in saline and transported in ice-box. While fresh goat lens were obtained from the frozen eye balls transported immediately from the slaughter house. Rat and rabbit lenses were dissected out by the method described by Haddad et al[6] for rabbits.

Lenses were carefully placed on a sterile tissue culture dish having a dark blue coloured nylon net, and were maintained either in tissue culture media T.C. 199, with or without either 20% human, rabbit or goat serum respectively;

or in a saline solution isotonic with aqueous. Rat lenses were maintained in isotonic saline and T.C. 199 without any serum. Experiments were carried out at either the pH of 7.2 or 7.8 and at room temperature (27° -29° C) or 37° C in an incubator.

Transparancy was measured by observing number and the characteristics of the squares seen through the lens. Lenses were observed for development of generalised haziness or opacities, intumescence or swelling, disruption and other morphological changes and weight gain. Changes were noted visually and recorded photographically.

Effect of partial and complete submersion of the lens in media fluid and placing on its anterior or posterior surface on the nylon grid was also studied.


  Observations Top


Lenses from all the four species could be maintained in either T.C. 199 with or without the addition of 20% serum or in isotonic saline for 3-7 days. Qualitatively similar and parellel pattern of change was seen.

The earliest change observed in a normal lens was an opacity starting peripherally and sometimes having a central cataract as well seen at the end of 30-40 hrs. Lens then showed swelling or intumuscence, as confirmed by thickening and distortion of lines of squares seen through the lens, with broadening of the squares and reduction in their number. As the opacity progressed (48-72 hrs) the lens became gradually transluscent and hazy. By the end of 72 hrs the lens developed full intumescence with a gain in weight of 100-500 mg and be­came completely opaque. It later started to disrupt and disintegrate by 96-120 hrs. The experiments were terminated by removing the lens from media fluid.

Rat lenses were least viable of the three animal species (3-4 days), rabbit lenses were more viable (4-5 days) and goat lenses were the most viable (5-7 days).

Lenses could be maintained at either pH of 7.2 or 7.8 and in both media or temperature without any significant effect on this pattern. However, if kept on anterior surface down or if submerged completely in culture media or saline, it hastened the above process of develop­ment of opacities by 2-3 days.


  Discussion Top


Our results indicate that lenses from other animal species like goats and rats in addition to rabbits can be used for lens culture. They could also be maintained at room temperature unlike most of the previous reports where they were maintained at 37°C in an incubator. It is interesting to note that when the lens was to­tally submerged it lost its transparancy much more rapidly than when its anterior surface remained facing the atmosphere. Also when the anterior surface of the lens faced the nylon grid there was a rapid development of opacities and disintegration indicating that either the posterior surface has some pump function diffe­rent from or may be of an opposite nature to that of anterior surface.

Lens culture can hence be used to study various transport and metabolic phenomenon in the lens and also as an experimental model for study of cataracts and for pharmacological evaluation of the drugs acting on lens specially and eye in general.


  Summary Top


Lenses of various species were maintained in either T.C. 199 media or in a saline solution made isotonic with aqueous humour. Effect of pH, temperature, media, anatomical position, submersion or exposure to air was studied.

It was noted that the lenses can be main­tained for 3-7 days in pH ranges of 7.2 to 7.8 in both the media, at 28-29°C (R.T.) or at 37°C. They should be placed on posterior sur­face and should not be submerged completely. The earliest changes start by 30-40 hrs. as an initial haziness, or peripheral opacities and which are followed by central cataract. Lenses swell by the end of 48-72 hrs. and usually dis­rupt and disintegrate by 96-120 hrs. These changes can be recorded visually as well as photographically.


  Acknowledgement Top


The authors thank the Dean, Dr. V.M. Medical College, Solapur for the facilities given to undertake this work.

The work was supported by research grant from Shivaji University, Kolhapur.

 
  References Top

1.
Kinsey, V.E., Wachtl, C., Constant, M.A., and Camachoe E., 1955, Amer. J. Ophthalmol., 40:216:  Back to cited text no. 1
    
2.
Constant, M.A., 1958, Amer. J. Ophthalmol., 45:372.  Back to cited text no. 2
    
3.
Harding, D.V., Rothstein, H., and Newman, M.B., 1962, Exp. Eye. Res. 1,457.  Back to cited text no. 3
    
4.
Schwartz, B., 1973, Culture of the lens, tissue culture Methods and Applications. New York, Acade­mic Press.  Back to cited text no. 4
    
5.
Mathur, S.P., 1976, Acta Sixth Afro-Asian Congress of Ophthalmol., 192-194.  Back to cited text no. 5
    
6.
Haddad, H.M., Shore, B., and Fuman M., 1967, Amer. J. Ophthalmol:., 63, 1731.  Back to cited text no. 6
    




 

Top
 
 
  Search
 
    Similar in PUBMED
   Search Pubmed for
   Search in Google Scholar for
    Access Statistics
    Email Alert *
    Add to My List *
* Registration required (free)  

 
  In this article
Materials and me...
Observations
Discussion
Summary
Acknowledgement
References

 Article Access Statistics
    Viewed2285    
    Printed91    
    Emailed5    
    PDF Downloaded0    
    Comments [Add]    

Recommend this journal