|Year : 1981 | Volume
| Issue : 3 | Page : 303-305
A study on transaminase level in aqueous humour
KK Sengupta1, AK Das1, R Bose2, A Chatterjee2, Ranabir Mukherji1
1 Department of Ophthalmology, Calcutta National Medical College, Calcutta, India
2 Department of Biochemistry, Calcutta National Medical College, Calcutta, India
K K Sengupta
Department of Ophthalmology, Calcutta National Medical College, Calcutta
Source of Support: None, Conflict of Interest: None
|How to cite this article:|
Sengupta K K, Das A K, Bose R, Chatterjee A, Mukherji R. A study on transaminase level in aqueous humour. Indian J Ophthalmol 1981;29:303-5
|How to cite this URL:|
Sengupta K K, Das A K, Bose R, Chatterjee A, Mukherji R. A study on transaminase level in aqueous humour. Indian J Ophthalmol [serial online] 1981 [cited 2020 Dec 1];29:303-5. Available from: https://www.ijo.in/text.asp?1981/29/3/303/30907
The enzymes, glutamic-oxalo-acetic transaminase (G.O.T.) and glutamic pyruvic transaminase (G.P.T.) are widely distributed in the cells of human body and destruction of any cell leads to leakage of these enzymes in the blood stream.
The aim of the present study is (i) to find out the normal level of G.O.T. and G.P.T. in aqueous humour and also (ii) to observe whether in conditions where G.O.T. and G.P.T. level is increased in blood, there is corresponding increase of these enzymes in aqueous humour.
| Materials and methods|| |
For the purpose of this study, the G.O.T. and G.P.T. content of blood and aqueous humour of 24 individuals were estimated. All these individuals were patients of the Eye Department of the Calcutta National Medical College, Hospital and underwent surgery for cataract. The subjects were divided into Groups A and B Group A comprising of 12 healthy individuals, in whom no disease other than cataract could be detected, constituted the normal control series. GroupB comprised of 12 individuals who were known sufferers of diseases like cirrhosis liver and connective tissue disorders. The samples of blood and aqueous from patients of both the series were collected during cataract surgery. The transaminase content in blood and aqueous was estimated by calorimetric technique of Mohum and Cook.
| Observations and discussions|| |
The G.O.T. and G.P.T. levels in aqueous and blood of individuals belonging to Group A and Group B is shown in [Table - 1][Table - 2] respectively.
It has been observed in the present study that the normal G.O.T. and G.P.T. level in aqueous is 24.3 + 0.7 1.U. /ml and 23.2 + 0.6 I U/ml. respectively [Table - 3]
[Table - 3] further reveal that transaminase level of aqueous is more or less constant in individuals of both Groups A and B although the blood level of these enzymes is much higher in the Group B individuals. Thus, there has been no appreciable rise in the G.O.T. level of aqueous humour even when these enzymes in blood are as higher as double its normal level (64 to 74 I.U./ml.).
| Summary|| |
The G.O.T. and G.P.T. levels of aqueous and blood were estimated in two Groups of individuals -one constituted the normal control series who had no systemic disease and the other group of patients who were known sufferers of diseases which cause high transaminase content in blood. When the results of both the groups were compared, it was observed that the transaminase level in aqueous remained within normal limits even in cases where there were high serum transaminase.
| Acknowledgements|| |
We are thankful to Dr. H.N. Ghosh, Principal-Superintendent, Calcutta National Medical College & Hospitals for kindly allowing us to carry out this work and to publish it.
| References|| |
Mohum, A.F. and Cook, I.J.Y., 1957, J. Clin. Path. 10 : 394.
[Table - 1], [Table - 2], [Table - 3]