|Year : 1983 | Volume
| Issue : 1 | Page : 17-19
Effect of estrogen on iris in the albino rat
KK Bisaria, Sharma Devi Bisaria
Department of Anatomy, King George's Medical College and Department of Ophthalmology, Vivekananda Polyclinic, Lucknow, India
K K Bisaria
Department of Anatomy, King George's Medical College, Lucknow
Source of Support: None, Conflict of Interest: None
|How to cite this article:|
Bisaria K K, Bisaria SD. Effect of estrogen on iris in the albino rat. Indian J Ophthalmol 1983;31:17-9
The effect of estrogen on iris in the albino rats is not reported in the available literature. However, its effects on other organs of the body like thyroid gland, ovary, liver, spleen, myocardium, coronary arteries etc. have been described by many workers.
The effect of estrogen on some parts of eye also have been described. Lipachutz mentioned about conjunctival tumorgenesis, Jonek on the effect on the DNA synthesis in corneal epithelium and Gierek on intraocular tension in the eyeballs of rabbits.
| Materials and methods|| |
In total 24 adult albino rats of either sex were used for this study. 6 were kept as control animal and the rest 18 were divided into 2 groups. One group was given estrogen (Neoclinestrol) 0.05 mg intramuscular injection daily for I week, 3 weeks and 5 weeks and another 0. 25 mg intra muscular injection for 1 week, 3 weeks and 5 weeks. The animals were sacrificed after these intervals by cutting the internal carotid artery under deep ether anaesthesia. The eyes were enucleated and kept in Bouin's fluid for 48 hours. These were then washed in running water for 1 hour. The eyes were then treated with graded alcohols. The paraffin blocks were prepared and sections were cut at 5 micron thickness. All slides were stained with haematoxylin and eosin.
| Observations|| |
Normal structure : The normal iris showed anteriorly stroma containing the sphincter and dilator pupillae, The sphincter was massed near the pupillary margin and dilator pupillae formed a single cell layer in front of pigment epithelium. The bilayered pigment epithelium were observed in all eyes posteriorly [Figure - 1].
Rats treated with 0.05 mg intramuscular injection of estrogen showed
After 1 and 3 weeks-No change
After 5 weeks-Blood vessels were congested in all the eyeballs of 3 rats.
Rats treated with 0.25 mg intramuscular injections of estrogen showed
After I week-In 1 rat blood vessels were congested and in rest 2 rats no change.
After 3 weeks-Blood vessels in all the 3 rats were congested. The posterior synechiae were observed in 2 rats [Figure - 2] and in 1 they were broken leaving pigments with exudate on anterior surface of lens [Figure - 3].
After 5 weeks-In 2 rats total posterior synechiae, were seen with gross atrophy [Figure - 4][Figure - 5], In 1 rat partial posterior synechiae with atrophy were noted.
| Discussion|| |
The estrogen alone or with other hormone is widely used now a days either for treatment or as contraceptive measures. It has been administered for the treatment of prostatic hypertrophy, oligospermia, pulmonary haemorrhage, osteoporosis, atherosclerosis, ovarian granulose cell tumor, male juvenile acne, bronchial asthma etc.
In the orbit, the effect of estrogen on Harder glands of albino rats, lens epithelium, cornea, conjunctiva have been described but its effect on iris has not been found in the available literature. In ihis work estrogen had little inflammatory effect in lower doses but in higher doses and for a long period of administration it showed profound inflammatory reaction followed by atrophy of iris tissue. It is thus suggested that during treatment with high doses of estrogen alone or with combination in various ailments, the eye examination for any evidence of iritis must be always kept in mind.
| Summary|| |
The effect of estrogen on the iris of male albino rats has not been reported in the past. In low dose of estrogen in early stage no changes in the iris were observed. In high dose even in early stage inflammatory changes were observed but in prolonged administration the postinflammatory atrophic changes in iris were noted.
| Acknowledgement|| |
We are greatly indebted to late Prof. D. Narayan, for his able guidance in the examination of microslides.
| References|| |
Lipachutz. L., 1941, L. Exper. Biol & Med. 48: 467
Jonek, J., 1967, Ginek. Pol, 38 : 117
Gierek, A., 1976, ophthalmologica. 172 : 400
[Figure - 1], [Figure - 2], [Figure - 3], [Figure - 4], [Figure - 5]