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ORIGINAL ARTICLE
Year : 1983  |  Volume : 31  |  Issue : 1  |  Page : 17-19

Effect of estrogen on iris in the albino rat


Department of Anatomy, King George's Medical College and Department of Ophthalmology, Vivekananda Polyclinic, Lucknow, India

Correspondence Address:
K K Bisaria
Department of Anatomy, King George's Medical College, Lucknow
India
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Source of Support: None, Conflict of Interest: None


PMID: 6629445

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How to cite this article:
Bisaria K K, Bisaria SD. Effect of estrogen on iris in the albino rat. Indian J Ophthalmol 1983;31:17-9

How to cite this URL:
Bisaria K K, Bisaria SD. Effect of estrogen on iris in the albino rat. Indian J Ophthalmol [serial online] 1983 [cited 2020 Nov 23];31:17-9. Available from: https://www.ijo.in/text.asp?1983/31/1/17/27425

The effect of estrogen on iris in the albino rats is not reported in the available literature. However, its effects on other organs of the body like thyroid gland, ovary, liver, spleen, myocardium, coronary arteries etc. have been described by many workers.

The effect of estrogen on some parts of eye also have been described. Lipachutz[1] mentioned about conjunctival tumorgenesis, Jonek[2] on the effect on the DNA synthesis in corneal epithelium and Gierek[3] on intraocular tension in the eyeballs of rabbits.


  Materials and methods Top


In total 24 adult albino rats of either sex were used for this study. 6 were kept as con­trol animal and the rest 18 were divided into 2 groups. One group was given estrogen (Neoclinestrol) 0.05 mg intramuscular injection daily for I week, 3 weeks and 5 weeks and another 0. 25 mg intra muscular injection for 1 week, 3 weeks and 5 weeks. The animals were sacrificed after these intervals by cutting the internal carotid artery under deep ether anaes­thesia. The eyes were enucleated and kept in Bouin's fluid for 48 hours. These were then washed in running water for 1 hour. The eyes were then treated with graded alcohols. The paraffin blocks were prepared and sections were cut at 5 micron thickness. All slides were stained with haematoxylin and eosin.


  Observations Top


Normal structure : The normal iris showed anteriorly stroma containing the sphincter and dilator pupillae, The sphincter was massed near the pupillary margin and dilator pupillae formed a single cell layer in front of pigment epithelium. The bilayered pigment epithelium were observed in all eyes posteriorly [Figure - 1].

Rats treated with 0.05 mg intramuscular injection of estrogen showed

After 1 and 3 weeks-No change

After 5 weeks-Blood vessels were conges­ted in all the eyeballs of 3 rats.

Rats treated with 0.25 mg intramuscular injections of estrogen showed

After I week-In 1 rat blood vessels were congested and in rest 2 rats no change.

After 3 weeks-Blood vessels in all the 3 rats were congested. The posterior synechiae were observed in 2 rats [Figure - 2] and in 1 they were broken leaving pigments with exudate on anterior surface of lens [Figure - 3].

After 5 weeks-In 2 rats total posterior synechiae, were seen with gross atrophy [Figure - 4][Figure - 5], In 1 rat partial posterior synechiae with atrophy were noted.


  Discussion Top


The estrogen alone or with other hormone is widely used now a days either for treatment or as contraceptive measures. It has been administered for the treatment of prostatic hypertrophy, oligospermia, pulmonary haemor­rhage, osteoporosis, atherosclerosis, ovarian granulose cell tumor, male juvenile acne, bronchial asthma etc.

In the orbit, the effect of estrogen on Har­der glands of albino rats, lens epithelium, cornea, conjunctiva have been described but its effect on iris has not been found in the available literature. In ihis work estrogen had little inflammatory effect in lower doses but in higher doses and for a long period of adminis­tration it showed profound inflammatory reac­tion followed by atrophy of iris tissue. It is thus suggested that during treatment with high doses of estrogen alone or with combination in various ailments, the eye examination for any evidence of iritis must be always kept in mind.


  Summary Top


The effect of estrogen on the iris of male albino rats has not been reported in the past. In low dose of estrogen in early stage no changes in the iris were observed. In high dose even in early stage inflammatory changes were observed but in prolonged administration the postinflammatory atrophic changes in iris were noted.


  Acknowledgement Top


We are greatly indebted to late Prof. D. Narayan, for his able guidance in the examina­tion of microslides.

 
  References Top

1.
Lipachutz. L., 1941, L. Exper. Biol & Med. 48: 467  Back to cited text no. 1
    
2.
Jonek, J., 1967, Ginek. Pol, 38 : 117  Back to cited text no. 2
    
3.
Gierek, A., 1976, ophthalmologica. 172 : 400  Back to cited text no. 3
    


    Figures

  [Figure - 1], [Figure - 2], [Figure - 3], [Figure - 4], [Figure - 5]



 

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