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Year : 1984  |  Volume : 32  |  Issue : 5  |  Page : 427-428

Role of glucose oxidase in combination with antibiotics on ocular pathogens

JL N. Medical College, Ajmer, India

Correspondence Address:
G K Sharma
J.L.N. Medical College, Ajmer
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Source of Support: None, Conflict of Interest: None

PMID: 6545334

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How to cite this article:
Sharma G K, Kabra S G, Bedi D, Nepalia L K. Role of glucose oxidase in combination with antibiotics on ocular pathogens. Indian J Ophthalmol 1984;32:427-8

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Sharma G K, Kabra S G, Bedi D, Nepalia L K. Role of glucose oxidase in combination with antibiotics on ocular pathogens. Indian J Ophthalmol [serial online] 1984 [cited 2022 Sep 27];32:427-8. Available from: https://www.ijo.in/text.asp?1984/32/5/427/27530

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The glucose is a diffusible but non-ionized constituent of lacrimal fluid. Since the pioneering work of Ridley,[1] several workers, including Gaur et al,[2] have attempted to quantitatively correlate the level of tear glucose with that of blood in diabetic states. Sharma et a1[3] observed an increase in glucose in tear fluid in ocular inflammations. This prompted the idea that if glucose, the nutrient for pathogenic organisms, is eliminated from tears, it should definitely help the antibiotics to have a better control over the infections.

A search in this regard by Sharma et a1[3] found 0.1% glucose oxidase to be innocuous in nature and effective to reduce the glucose contents of the tears. An experimental study was, therefore, designed to evaluate the effect of 0.1% glucose oxidase alone, in combination with antibiotics and antibiotics alone, on the control of the growth of glucose utilizing pathogens.

  Material and methods Top

This study was carried out on the organisms isolated from 25 cases each of acute conjunctivitis and corneal ulcers.

These isolated organisms were tested for sensitivity to various antibiotics alone, antibiotics with glucose oxidase and glucose oxidase alone, in terms of zone of inhibition in milimeters.

Antibiotics selected for this study were penicillin, chloramphenicol, terramycin and gentamycin sulphate in the strength of 100 units per disc, 50 ug per disc, 25 ug per disc and 10 ug per disc respectively.

Discs which were impregnated with antibiotics and glucose oxidase had the same amount of antibiotics, in addition there were 0.1 mg of glucose oxidase per disc and the next set of discs had only 0.1 mg per disc of glucose oxidase as control.

  Observations and discussion Top

Results of isolation of pathogenic organisms from 25 cases each of acute con­junctivitis and corneal ulcers are presented in [Table - 1].

The comparison of inhibition of bacterial growth between the antibiotics used alone and in conjunction with glucose oxidase is presented in [Table - 2].

Because of the variability of growth zone of the different isolated organisms in each group, a correlation analysis of the quantum of inhibition between the components of each group, was considered of greater relevance than simple statistical difference in the means of two groups.

The results reveal that quantum of inhibi­tion is significantly higher and bears a significant correlation with the addition of glucose oxidase to the antibiotics. It is, therefore, clear that addition of glucose oxidase has a signifi­cant potentiating effect with all the antibiotics in all the pathogens tested.

In three cases of penicillin resistant organisms, interestingly addition of glucose oxidase showed an appreciable zone of inhibition. viz., 10, 12 and 16 mm in com­parison to zero with penicillin alone. This however, resulted in anomalous variability that rendered the correlation analysis statis­tically insignificant As a matter of fact, however, these three cases reveal a highly significant neutralization of the resistance of the organisms to penicillin by glucose oxidase.

  Summary Top

There is unequivocal evidence that glucose oxidase used in conjunction with antibiotics, significantly enhances their antibacterial property.

  References Top

Ridley F., 1930. Brit. J. Exp. Path., 11: 217.  Back to cited text no. 1
Gaur M., Sharma G.K., Kabra S.G., Sharma P.R and Nepalia L.K., 1982. Ind. J. Ophthalmol. Vol 30: 367.  Back to cited text no. 2
Sharma G.K., Kabra S.G., Sharma P.K., Napalia LK., Bhardwaj S., and Mehra A,1982. Ind. J. Ophthalmol. Vol. 31: 563.  Back to cited text no. 3


  [Table - 1], [Table - 2]


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