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Year : 1992  |  Volume : 40  |  Issue : 1  |  Page : 11-14

Effect of SH reagents on rod photoresponse of isolated frog retina

1 Division of Molecular Biology, Department of Biophysical Engineering, Faculty of Engineering Science, Osaka University, Toyonaka, Otsaka 560, Japan
2 Department of Biology, College of General Education, Osaka University, Toyonaka, Osaka 560, Japan
3 Department of Biological and Chemical Engineering, Gunma University, Kiryu, Gunma 376, Japan
4 Department of Physiology, Kinki University School of Medicine, Osakasayama-shi 589, Japan

Correspondence Address:
T Shinozawa
Department of Biology. College of General Education, Osaka University, Toyonaka, Osaka 560
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Source of Support: None, Conflict of Interest: None

PMID: 1464446

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Several SH reagents, N-ethylmaleimide (NEM), p-chloromercuribenzoic acid (PCMB), p-chloromercuribenzene sulphonate (PCMBS) and monoiodoacetic acid (MIAA) changed the wave form and the peak of the amplitude of the photoresponse remarkably. The effects of amino group modifying reagents, ethyl acetimidate (EA) and isethinyl acetimidate (ITA) on photoresponse were very slight. The possibility of a SH protein as cGMP-sensitive cation channel protein is discussed.

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