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   Table of Contents      
ORIGINAL ARTICLE
Year : 2014  |  Volume : 62  |  Issue : 5  |  Page : 545-548

Deep anterior lamellar keratoplasty with a manual spatula: Anatomical and functional results


Division of Cornea and External Disease, Clinics Hospital, University of São Paulo, HCFMUSP-SP, Division of Refractive Surgery of UNICO SP, Brazil

Date of Submission31-Mar-2013
Date of Acceptance14-Feb-2014
Date of Web Publication30-May-2014

Correspondence Address:
Mário Henrique Camargos de Lima
Rua João Moura 945, apto 73, Pinheiros, São Paulo- SP, 05412002
Brazil
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Source of Support: None, Conflict of Interest: None


DOI: 10.4103/0301-4738.133483

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  Abstract 

Objective: Evaluate the anatomical, refractive, and functional results of an innovative technique of deep anterior lamellar keratoplasty with a manual spatula. Materials and Methods: We evaluated the results and examinations of 16 eyes from 14 patients who underwent deep anterior lamellar keratoplasty using the technique described by Ferrara. Residual bed thickness after keratoplasty was evaluated postoperatively using Visante. The measurement was performed using a technique similar to that used to measure flap thickness after laser in situ keratomileusis refractive surgery. The measurements were performed at the center of the cornea in an area comprising the central 3-mm in the 45° and 135° meridians. Results: Best-corrected visual acuity was 0.34 ± 0.18 LogMar (0.09 to 0.60 LogMar), the spherical equivalent was -4.31 ± 3.38 D (+0.25 to -9.50 diopters), and keratometry was 45.75 ± 2.77 D (41.11 to 52.48 diopters) postoperatively. Corneal astigmatism was 3.19 ± 2.78 D (0.18 to 11.81 diopters). Residual stromal bed thickness measured by optical coherence tomography showed values of 67.1 ± 24.3 μm (30 to 109 μm). The statistical correlation by Spearman's test between the best-corrected visual acuity and the residual stromal bed thickness was 0.11 (P = 0.67). Conclusion: Deep anterior lamellar keratoplasty, in which manual dissection was performed using an instrument similar to that used to implant corneal rings, provided good visual and anatomical results.

Keywords: Cornea, corneal transplantation, eye diseases, keratoconus, ophthalmology


How to cite this article:
de Lima MC, Rizzi AR, Simoceli RA, Cresta FB, Alves MR. Deep anterior lamellar keratoplasty with a manual spatula: Anatomical and functional results. Indian J Ophthalmol 2014;62:545-8

How to cite this URL:
de Lima MC, Rizzi AR, Simoceli RA, Cresta FB, Alves MR. Deep anterior lamellar keratoplasty with a manual spatula: Anatomical and functional results. Indian J Ophthalmol [serial online] 2014 [cited 2020 Nov 30];62:545-8. Available from: https://www.ijo.in/text.asp?2014/62/5/545/133483

Deep anterior lamellar keratoplasty (DALK) is a technique for partial thickness corneal transplantation that preserves endothelial cells after removing all or part of the stroma, resulting in a less thick but uniform residual bed. [1]

The advantages of using this type of keratoplasty are numerous when compared to penetrating keratoplasty. First, it is conducted on a closed eye, which decreases the risk of complications such as the formation of synechiae, glaucoma, and endophthalmitis. [1],[2] DALK may also have increased tectonic strength and superior resistance to rupture of the globe after blunt trauma. [3],[4]

Moreover, maintenance of the recipient endothelial cells eliminates complications related to endothelial rejection, which is a major cause of penetrating keratoplasty failure. [5] Corticosteroids have an important role in avoid stromal rejection in these patients. Nevertheless, some studies suggest that there is no need to use prolonged immunosuppressive therapy, particularly corticosteroids, which minimizes the risk of cataract, glaucoma, and postoperative infection. [1],[5]

The literature shows similar visual acuity results for DALK compared with penetrating keratoplasty. [6],[7],[8]

The use of the big bubble technique has improved the outcome of DALK with respect to residual thickness of the stroma; [1],[2] however, there is no consensus on the visual function achieved in eyes in which variable thickness of the posterior stroma is preserved. [9],[10]

A steep learning curve is associated with the DALK procedure. The most common intraoperative complication is perforation of Descemet's membrane, which requires conversion to penetrating keratoplasty. To avoid this complication, surgeons seek to carry out techniques that preserve the minimum thickness of the posterior stroma. [11],[12],[13] The use of a manual dissection technique for deep stroma with an instrument developed by Ferrara to implant corneal rings requires a shallower learning curve and offers greater regularity of the residual stroma. [14]

This study evaluated the anatomical and functional results of DALK performed using a manual tunneler and correlated the residual stromal bed thickness results with those of best-corrected visual acuity (BCVA).


  Materials and Methods Top


We studied a series of cases with intervention to evaluate the anatomical and functional results of 16 eyes from 14 patients who underwent DALK using the manual dissection technique and the instrument developed by Ferrara. [14] This study was approved by the Ethics Committee of the Faculty of Medicine, University of São Paulo, São Paulo, SP, Brazil.

Patients included in the study were diagnosed with keratoconus, defined by the presence of at least one of the following criteria: Slit lamp changes (Vogt striae or Fleischer ring), central power > 48.7 D, and the difference between corneal power in the lower and upper cornea in the area of 3-mm (IS) >1.9 D. The patients were 21-41 years of age with BCVA (glasses or contact lenses) ≤20/80 (0.60 LogMar) and with no deep corneal scarring or previous hydrops episodes. We excluded patients with other ocular co-morbidities such as cataract, glaucoma, retinal disease, high myopia, amblyopia, or previous ocular surgery.

All surgeries were performed by one surgeon (RA Simoceli) under peribulbar anesthesia and sedation. The surgical technique shared some steps with intrastromal corneal ring implantation. A corneal incision was made at 12 pm with a diamond knife at a 90% depth in the 8-mm optical zone [Figure 1]. Ultrasonic pachymetry was performed intraoperatively in the 90° axis to calculate the depth of the incision [Figure 1]. The incision was enlarged with a blunt instrument [Figure 2], and a manual spatula was inserted to make a tunnel similar to that made for intrastromal corneal ring implantation [Figure 2]. The spatula was equipped with a 6-0 nylon thread at its end and was inserted into the tunnel [Figure 3]. After tunneling, the spatula was removed and the nylon thread was pulled from both sides of the radial incision to obtain a deep dissection of the corneal stroma leaving the least possible residual stroma [Figure 4]. Trephine and scissors were used to remove the corneal flap and to regularize the bed for suturing. The size of excised corneal button has the same size of the spatula, 8 mm in all eyes. The donor cornea button was trepanned, 0, 5-mm larger than the recipient site, and without removing Descemet's membrane and the endothelium, was sutured with 16 equidistant nylon 10-0 sutures [Figure 5].
Figure 1: A corneal incision was made at 12 pm with a diamond knife at a 90% depth in the 8-mm optical zone

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Figure 2: The incision was enlarged with a blunt instrument, and a manual spatula was inserted to make a tunnel similar to that made for intrastromal corneal ring implantation

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Figure 3: A manual spatula was inserted to make a tunnel similar to that made for intrastromal corneal ring implantation. The spatula was equipped with a 6-0 nylon thread at its end and was inserted into the tunnel

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Figure 4: After tunneling, the spatula was removed and the nylon thread was pulled from both sides of the radial incision to obtain a deep dissection of the corneal stroma leaving the least possible residual stroma

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Figure 5: Trephine and scissors were used to remove the corneal flap and to regularize the bed for suturing. The donor cornea button was trepanned, 0, 5-mm larger than the recipient site, and without removing Descemet's membrane and the endothelium, was sutured with 16 nylon 10-0 equidistant sutures

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Results were obtained by ophthalmologic examination performed on all 14 patients (16 eyes) at 6-8 months postoperatively when the sutures had been removed. For each participant, we recorded measurement values ​​of BCVA in LogMar, refractive error expressed by the spherical equivalent, spherical diopters of the central corneal curvature using the Eye Sys topographer, and thickness of the residual bed measured with optical coherence tomography (OCT) of the anterior segment (Visante, Carl Zeiss Meditec, Dublin, CA, USA) using standard procedures similar to those validated for measurement of flap thickness after LASIK refractive surgery. [15] Measurements of the residual thickness of the stroma were performed in the center of the cornea in a zone comprising the central 3-mm of the 45 th meridian (including the 225 th ) and the 135 th meridian (including the 315 th ). A total of 10 measurements were made (five in each meridian), and the results were expressed as the mean of the measurements in microns [Figure 6].
Figure 6: The measurement was performed using a technique similar to that used to measure flap thickness after laser in situ keratomileusis refractive surgery. The measurements were performed at the center of the cornea in an area comprising the central 3-mm in the 45° and 135° meridians

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We used SPSS ver. 17.0 for statistical analysis (SSPSS Inc., Chicago, IL, USA). Quantitative data are presented as means and standard deviation. We evaluated the correlation between the quantitative variables (thickness of the stroma and residual better AVCC) by calculating Spearman's r coefficient and the P value. The significance level for the tests was 5%.


  Results Top


We had a predominance of male patients (78.5%) compared to females (21.5%). The preoperative BCVA was 1.21 ± 0.44 (0.18-2.1) LogMar. No micro or macro perforations occurred during the surgeries.

Mean values​​, standard deviations, and variations in BCVA were 0.34 ± 0.18 LogMar (LogMar 0.09 to 0.60), spherical equivalent of the refractive error -4.31± -3.38 D (+0 25 to -9.50 D), astigmatism 3.19 ± 2.78 D (0.18 to 11.81 D). The keratometry was 45.75 ± 2.77 D (41.11 to 52.48 diopters) postoperatively. The thickness of the residual central stroma showed values of 67.1 ± 24.3 μm [Table 1]. The thinnest total thickness measured by Visante in the 0-2 mm zone was 534.25 ± 55 μm (407 to 608 μm). Residual stromal bed thickness was < 80 μm in 11 eyes (68.75%).
Table 1: The chart shows the patient's ages (years), best corrected visual acuity

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The correlation between the bed thickness of the central stroma and the BSCVA was 0.11 (Spearman's r, P ≥ 0.67).


  Discussion Top


DALK presents many advantages over penetrating keratoplasty (e.g. preservation of corneal endothelial cells with decreased rates of rejection) possibility due to the low corneal endothelial cell counts and closed-eye surgery, which reduces perioperative complications. [16],[17] The main disadvantage is that the learning curve is longer. [18]

No micro or macro-perforations were observed in the present study. The Descemet's perforation is a complication that occurs in ISCR implantation, especially during the incision with the diamond knife. Nevertheless, this small Descemet's perforation does not require the conversion to PK, and the technique can be performed without major problems.

Bahar et al. 13 compared the results of 17 patients who underwent DALK with 27 patients who underwent penetrating keratoplasty and did not obtain a significant difference in BCVA. They reported that the average BCVA was 20/40 for DALK and 20/30 for penetrating keratoplasty during the first 12 months. Despite these differences not being significant, most studies show a slight trend toward better vision following penetrating keratoplasty compared to DALK. [8],[19]

The Anwar and Melles techniques of DALK have comparable visual acuity and refractive outcomes. [20] The BCVA values ​​obtained were considered good, as 12 eyes (75%) obtained a BCVA ≥ 0.5 logMAR (20/63), which is considered acceptable even after penetrating keratoplasty. [1],[7],[8] Our technique showed comparable CDVA with Anwar and Melles techniques. Further controlled studies comparing the techniques are necessary to clarify the difference between them.

The postoperative values ​​of the spherical equivalent of the refractive error and topographic astigmatism in the present study were considered acceptable when compared with postoperative values ​​for penetrating keratoplasty. [7],[8]

Deep stromal dissection performed using a wire facilitated removal of the posterior stromal tissue, which was corroborated by the finding that the residual stromal beds in 11 eyes (68.75%) were ≤80 μm. The wire dissection provided a regular stromal bed, and the thickness measures showed a slight tendency to be thicker in the periphery. Nonetheless, the central bed thickness measures were lower than 80 μm, providing a good visual result. Ardjomand et al. evaluated the residual stromal bed after lamellar keratoplasty in 14 patients and found that most patients with values < 80 μm achieved acceptable vision postoperatively. [21]

Despite the procedure does not create a bare Descemet's membrane, the interface haze was not an issue in any evaluated case. The amount of residual bed thickness (67.1 ± 24.3 μm) provided a BCVA similar to other techniques. The regularity of the stromal bed thickness produced by the nylon thread traction may explain that result.

We found no correlation between postsurgical BCVA and the thickness of the residual stromal bed (P ≥ 0.67). This finding may be explained by other variables, such as keratometry, final spherical equivalent, and irregular astigmatism, which may have influenced postoperative BCVA.


  Conclusion Top


DALK using the manual tunneling technique provided good visual and anatomical results. DALK using this technique can be considered an alternative procedure due to its simplicity and low incidence of complications.

 
  References Top

1.
Vajpayee RB, Tyagi J, Sharma N, Kumar N, Jhanji V, Titiyal JS. Deep anterior lamellar keratoplasty by big-bubble technique for treatment corneal stromalopacities. Am J Ophthalmol 2007;143:954-7.  Back to cited text no. 1
    
2.
Unal M, Arslan OS, Atalay E, Mangan MS, Bilgin AB. Deep Anterior Lamellar Keratoplasty for the Treatment of Stromal Corneal Dystrophies. Cornea 2013;32:301-5.  Back to cited text no. 2
    
3.
Reinhart WJ, Musch DC, Jacobs DS, Lee WB, Kaufman SC, Shtein RM. Deep anterior lamellar keratoplasty as an alternative to penetrating keratoplasty: A report by the American Academy of Ophthalmology. Ophthalmology 2011;118:209-18.  Back to cited text no. 3
    
4.
Borderie VM, Sandali O, Bullet J, Gaujoux T, Touzeau O, Laroche L. Long-term results of deep anterior lamellar versus penetrating keratoplasty. Ophthalmology 2012;119:249-55.  Back to cited text no. 4
    
5.
Olson EA, Tu EY, Basti S. Stromal rejection following deep anterior lamellar keratoplasty: Implications for postoperative care. Cornea 2012;31:969-73.  Back to cited text no. 5
    
6.
Huang T, Zhang X, Wang Y, Zhang H, Huand A, Gao N. Outcomes of deep anterior lamellar keratoplasty using the big-bubble technique in various corneal diseases. Am J Ophthalmol 2012;154:282-9.  Back to cited text no. 6
    
7.
Söðütlü Sari E, Kubaloðlu A, Ünal M, Piñero Llorens D, Koytak A, Ofluoglu AN, et al . Penetrating keratoplasty versus deep anterior lamellar keratoplasty: Comparison of optical and visual quality outcomes. Br J Ophthalmol 2012;96:1063-7.  Back to cited text no. 7
    
8.
Yeung SN, Lichtinger A, Kim P, Amiran MD, Rootman DS. Retrospective contralateral study comparing deep anterior lamellar keratoplasty with penetrating keratoplasty: A patient's perspective. Can J Ophthalmol 2012;47:360-4.  Back to cited text no. 8
    
9.
Sugita J, Kondo J. Deep lamellar keratoplasty with complete removal of pathological stroma for vision improvement. Br J Ophthalmol 1997;81:184-8.  Back to cited text no. 9
    
10.
Marchini G, Mastropasqua L, Pedrotti E, Nubile M, Ciancaglini M, Sbabo A. Deep lamellar keratoplasty by intracorneal dissection: A prospective clinical and confocal microscopic study. Ophthalmology 2006;113:1289-300.  Back to cited text no. 10
    
11.
Rama P, Knutsson KA, Razzoli G, Matuska S, Viganò M, Paganoni G. Deep anterior lamellar keratoplasty using an original manual technique. Br J Ophthalmol 2013;97:23-7.  Back to cited text no. 11
    
12.
Ghanem RC, Ghanem MA. Pachymetry-guided intrastromal air injection ("pachy-bubble") for deep anterior lamellar keratoplasty. Cornea 2012;31:1087-91.  Back to cited text no. 12
    
13.
Lange AP, Moloney G, Arino M, Ng A, McCarthy JM, White VA, et al. Enzyme-Assisted Deep Anterior Lamellar Keratoplasty-A New Method of Lamellar Dissection-A Wetlab-Based Pilot Study. Cornea 2013;32:98-103.  Back to cited text no. 13
    
14.
Ferrara P, Torquetti L, Cunha L. New technique of Deep Anterior Lamellar Keratoplasty. J Emmetropia 2011;2:79-83.  Back to cited text no. 14
    
15.
Ju WK, Lee JH, Chung TY, Chung ES. Reproducibility of LASIK Flap Thickness Using the Zeiss Femtosecond Laser Measured Postoperatively by Optical Coherence Tomography. J Refract Surg 2011;27:106-10.  Back to cited text no. 15
    
16.
Javadi MA, Feizi S, Yazdani S, Mirbabaee F. Deep anterior 
lamellar keratoplasty versus penetrating keratoplasty for 
keratoconus: a clinical trial. Cornea 2011;29:365-71.  Back to cited text no. 16
    
17.
Feizi S, Javadi MA, Kanavi MR. Cellular changes of donor corneal tissue after deep anterior lamellar keratoplasty versus penetrating keratoplasty in eyes with eratoconus: A confocal study. Cornea 2011;29:866-70.  Back to cited text no. 17
    
18.
Reinhart WJ, Musch DC, Jacobs DS, Lee WB, Kaufman SC, Shtein RM. Deepanterior lamellar keratoplasty as an alternative to penetrating keratoplasty a report by the American academy of ophthalmology. Ophthalmology 2011;118:209-18.  Back to cited text no. 18
    
19.
Bahar I, Kaiserman I, Srinivasan S, Ya-Ping J, Slomovic AR, Rootman DS. Comparison of three different techniques of corneal transplantation for keratoconus. Am J Ophthalmol 2008;146:905-12.  Back to cited text no. 19
    
20.
Baradaran-Rafii A, Eslani M, Sadoughi MM, Esfandiari H, Karimian F. Anwar versus Melles Deep Anterior Lamellar Keratoplasty for Keratoconus, Ophthalmology 2013;120:252-9.  Back to cited text no. 20
    
21.
Ardjomand N, Hau S, McAlister JC, Bunce C, Galaretta D, Tuft SJ, et al. Quality of vision and graft thickness in deep anterior lamellar and penetrating corneal allografts. Am J Ophthalmol 2007;143:228-35.  Back to cited text no. 21
    


    Figures

  [Figure 1], [Figure 2], [Figure 3], [Figure 4], [Figure 5], [Figure 6]
 
 
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