|Year : 2016 | Volume
| Issue : 5 | Page : 369-375
Vitreoretinal lymphomas misdiagnosed as uveitis: Lessons learned from a case series
Luca Cimino1, Marco Coassin2, Chi-Chao Chan3, Sylvia Marchi1, Matteo Belpoliti2, Andrea Fanti2, Alfonso Iovieno2, Luigi Fontana2
1 Department of Ophthalmology, Uveitis Service, Santa Maria Nuova Hospital-IRCCS, Reggio Emilia, Italy
2 Department of Ophthalmology, Santa Maria Nuova Hospital-IRCCS, Reggio Emilia, Italy
3 Laboratory of Immunology, National Eye Institute, National Institutes of Health, Bethesda, MD, USA
|Date of Submission||20-Jul-2015|
|Date of Acceptance||28-Apr-2016|
|Date of Web Publication||6-Jul-2016|
Dr. Luca Cimino
Department of Ophthalmology, Uveitis Service, Santa Maria Nuova Hospital-IRCCS,Viale Risorgimento, 80 42123 Reggio Emilia
Source of Support: None, Conflict of Interest: None
Purpose: To present challenging cases of vitreoretinal lymphoma (VRL) that was misdiagnosed as uveitis because of the apparent intraocular inflammation. At the light of the new classification of intraocular lymphomas, we detail the characteristics that masqueraded the tumors and the clinical aspects that guided us to the correct diagnosis. Materials and Methods: We retrospectively reviewed the patients referred to our uveitis service between January 2006 and December 2014. Results: Seven patients referred with a presumptive diagnosis of idiopathic uveitis received a final diagnosis of VRL. The median time between the onset of symptoms and definitive diagnosis was 25 months for these complex cases. The median time from presentation at our clinic to final diagnosis was 1 month. The described clinical features including dense vitreous cells and subretinal infiltrates were characteristic and tend to be present in all these chronically ill patients. Vitreous samples were collected, and all demonstrated the pathognomonic tumor cells, the specific immunoglobulin heavy chain gene rearrangements, and an interleukin (IL)-10 to IL-6 ratio >1. Conclusion: VRLs are severe diseases with a poor prognosis that may be misdiagnosed as idiopathic inflammatory conditions of the eye. Treatment with steroids may occult the tumors and delay the correct diagnosis. Appropriate evaluation may prompt to a timely vitreous sampling and therefore to a faster diagnosis in these peculiar cases where the correct diagnosis was delayed by several months.
Keywords: Diagnostic vitrectomy, intraocular inflammation, intraocular lymphoma, subretinal infiltrates, uveitis, vitreoretinal lymphoma
|How to cite this article:|
Cimino L, Coassin M, Chan CC, Marchi S, Belpoliti M, Fanti A, Iovieno A, Fontana L. Vitreoretinal lymphomas misdiagnosed as uveitis: Lessons learned from a case series. Indian J Ophthalmol 2016;64:369-75
|How to cite this URL:|
Cimino L, Coassin M, Chan CC, Marchi S, Belpoliti M, Fanti A, Iovieno A, Fontana L. Vitreoretinal lymphomas misdiagnosed as uveitis: Lessons learned from a case series. Indian J Ophthalmol [serial online] 2016 [cited 2021 Jan 17];64:369-75. Available from: https://www.ijo.in/text.asp?2016/64/5/369/185600
Intraocular lymphomas are one of the most critical entities to take into consideration in the differential diagnosis of patients with apparent ocular inflammation referred to tertiary uveitis clinics. ,,, Presenting rarely to the uveitis specialist,  diagnosis is often delayed, and prognosis can be very poor in these forms of intraocular lymphomas. ,,,,
The traditional classification, distinguishing lymphomas in a primitive subtype arising in the eye (primary intraocular lymphoma) and in a secondary subtype that metastasizes to the eye from a primary site, has been recently updated. Intraocular lymphomas are now divided into vitreoretinal and uveal forms. ,,,,
Vitreoretinal lymphomas (VRLs) are aggressive tumors that may interest the central nervous system (CNS). ,, Often bilateral, they clinically present with vitreous haze and a yellowish tissue infiltrating the subretinal space. Vitreous opacities, retinal infiltrates, and the possible presence of iritis or keratic precipitates may mislead to a diagnosis of uveitis. , VRLs are usually extranodal, non-Hodgkin, diffuse, large B-cell type lymphomas that belong to the family of primary CNS lymphomas (although also T-cell VRLs have been described). ,, The incidence of these tumors in immunocompetent patients is increasing. 
Uveal lymphomas include primary and secondary forms. , Primary lymphomas of the choroid were previously called reactive lymphoid hyperplasia because of their low-grade nature. Secondary uveal lymphomas are metastatic localizations to the choroid in patients affected by non-Hodgkin lymphoma in other organs.
Patients with intraocular malignancies are usually referred to the ocular oncology service. We herein describe a number of challenging cases that were not immediately recognized as neoplasm and were referred to our uveitis service for evaluation of the apparent intraocular inflammation. , The purpose is to present the clinical aspects that masqueraded the tumors and delayed the diagnosis, putting at risk the lives of the patients.
| Materials and Methods|| |
We retrospectively reviewed the electronic medical records of all patients referred to the uveitis service of our hospital between January 1, 2006, and December 31, 2014. All immunocompetent patients with a presumptive diagnosis of uveitis underwent an extensive work-up as previously described  and were classified according to the criteria of the International Uveitis Study Group.  The Ethical Committee of our hospital approved this clinical research protocol.
The patients with a final diagnosis of VRL were individuated. We collected from their medical records: Patient demographics including age, gender, and race; previous medical history; symptoms of initial presentation; signs and clinical characteristics of the ocular disease; time between the onset of symptoms to definitive diagnosis of lymphoma; the specific work-up including the surgical procedures; and the methods of histopathologic and molecular diagnosis.
Undiluted vitreous samples were collected and processed as previously recommended. ,,, Briefly, vitreous specimen was immediately centrifuged. The supernatant was removed and used for cytokine analysis by ELISA assay. The precipitant was used for cytology; the cells were placed on the coated slides and prepared for Giemsa stain. Supernatants and coated slides were immediately sent to the Laboratory of Immunology of the National Eye Institute (National Institutes of Health, Bethesda, Maryland, USA) for cytology and molecular analysis. ,, The levels of interleukin (IL) 6 and 10 were measured to differentiate between inflammatory and neoplastic diseases.  Microdissection techniques with polymerase chain reaction (PCR) amplification were used to detect monoclonality of the malignant B-cells and specifically, the rearrangements of the immunoglobulin heavy chain (IgH) gene, as previously described. ,,
| Results|| |
One thousand and three hundred patients with a presumed new diagnosis of uveitis were seen in our tertiary center between January 2006 and December 2014. Seven cases (0.54%) had a diagnosis of VRL after pars plana vitrectomy (PPV). The age of patients ranged from 45 to 86 years (mean 67.8 years), 4 were males and 3 females, and they were all Caucasian [Table 1].
|Table 1: Description of history, clinical findings, molecular analysis, and time to diagnosis in cases with vitreoretinal lymphomas|
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The median time between the onset of symptoms and definitive diagnosis was 25 months (interquartile range: 8.5-28 months). The median time from presentation at our clinic to final diagnosis was 1 month. Six patients had a confirmed diagnosis of intraocular lymphoma within 1 month from presentation to our institution, whereas it took 13 months for one patient (the first of the series).
At the ophthalmic evaluation, all patients presented with vitreous haze and subretinal lesions in both eyes [Figure 1] and [Figure 2]. Three patients had in addition anterior chamber signs such as flare, cells, fibrin, or keratic precipitates. One patient showed serous retinal detachment and choroidal thickening. Four patients did initially respond to systemic corticosteroids.
|Figure 1: (a) Clinical fundus picture showing a very aggressive form of vitreoretinal lymphoma: Mild vitreous haze and subretinal yellowish infiltrates. (b) Clinical fundus picture showing a very aggressive form of vitreoretinal lymphoma: 1 week later the lesions are rapidly growing and retinal hemorrhages are present|
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|Figure 2: The subretinal pigment epithelium localization of the tumor cells in vitreoretinal lymphoma are well demonstrated by optical coherence tomography|
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The seven patients came to our attention with a presumptive diagnosis of idiopathic uveitis. The final diagnosis was primary VRL in all the cases, and their cellular type was diffuse large B-cell non-Hodgkin lymphoma.
Two patients had a previous CNS lymphoma before the intraocular form. Case 1 had a CNS lymphoma cured 2 years before the diagnosis of the ocular disease. Case 2 was successfully treated for cerebral lymphoma 15 years before the diagnosis of intraocular lymphoma. Both patients had negative brain magnetic resonance imaging (MRI) and were considered in clinical remission from the previous systemic disease when we performed the diagnostic vitrectomy because of the ocular signs.
Case 5 received the diagnosis of cerebral and ocular lymphoma at the same time (7 months after the initial ocular symptoms). The other four patients with VRL did not show any sign of CNS disease.
All patients had the diagnosis of lymphoma confirmed by cytology, showing large atypical lymphoid cells with scant cytoplasm, segmented nuclei, and prominent nucleoli [Figure 3] and [Figure 4]. IgH gene rearrangement was confirmed in all patients. The concentrations of IL-6 and IL-10 in the vitreous are summarized in [Table 1]. The mean levels of IL-6 and IL-10 were 483.5 pg/ml and 15,977.3 pg/ml, respectively, with an IL-10/IL-6 ratio >1 in 100% of cases.
|Figure 3: Cytology showing vitreoretinal lymphoma cells with large irregular nuclei, prominent nucleoli, and scant basophilic cytoplasm (×400)|
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| Conclusion|| |
The incidence of ocular lymphomas has increased in the past years,  and patients may be referred to the uveitis specialist because these tumors may mimic inflammatory eye disease. ,,,
Prognosis is severe and diagnosis difficult and often delayed. , In this series, the median time from symptoms to final diagnosis was 25 months. A median time of 1 month was necessary to reach a conclusive diagnosis since they were referred to our institution. Rapid diagnoses of ocular lymphoma may be obtained taking into consideration some characteristics that increase clinical suspicion.
The first of these characteristics is the age of the patient referred for presumed uveitis. In our series, the mean age at diagnosis of ocular lymphoma was 64.4 years. This data are in line with other reports  and significantly higher than the age of patients diagnosed with uveitis (average 31.1 years at diagnosis in a similar population  ).
Clinical characteristics should also be carefully considered. VRLs usually affect both eyes. Although many diseases have been classically considered in the differential diagnosis of VRLs, the characteristic subretinal deposits are quite striking [Figure 1] and [Figure 2]. All our patients with primary VRL presented with vitreous haze and subretinal yellowish deposits, sometimes rapidly enlarging. Patients usually complain of blurred vision and/or floaters [Figure 5], [Figure 6], [Figure 7], [Figure 8]. Extraocular symptoms are rare, but patients should be questioned for ataxia, dizziness, headaches, or low-grade fever. 
|Figure 5: Case 1 right (a) and left (b) eye: vitreoretinal lymphoma, acute phase (diffuse subretinal yellowish lesions). At bottom of (b) we see two left eye fluoroangiography pictures. (c and d) Right and left eye: Convalescent phase|
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|Figure 6: (a and b) Case 3: Primary vitreoretinal lymphoma in the right eye, at diagnosis and after 1 month therapy (intravitreal methotrexate and systemic chemotherapy [b])|
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|Figure 7: (a and b) Case 4: Bilateral primary vitreoretinal lymphoma (subretinal yellowish lesions at posterior pole and vitreous infiltration more evident in the right eye), at diagnosis (right [a] and left [b] eye)|
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|Figure 8: (a and b) Case 5: Bilateral vitreoretinal lymphoma, faint subretinal infiltrates, mild vitritis (at presentation). (c) Positron emission tomography scan shows brain lesion (at presentation). (d and e) Right and left eye in the convalescent phase|
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The standard work-up for infectious and noninfectious uveitis is usually unremarkable. In the majority of the cases, this prompts the diagnosis of idiopathic uveitis and treatment with systemic steroids. As described in previous papers, this might severely delay the final diagnosis. ,, A temporary, intermittent, or incomplete response to steroids should raise suspicion of intraocular lymphoma. As shown by our series, when chest X-ray, lumbar puncture, brain MRI, or total body positron emission tomography scans are negative, the diagnosis of intraocular lymphoma is still possible. If VRL lymphoma is present, a vitreous sample obtained by pars plana vitrectomy is virtually the only way to confirm the diagnosis.
If obtained, vitreous samples should be manipulated and preserved properly. ,,, If an expert cytopathologist is not available locally, arrangements should be made before surgery and the material should be safely sent to a specialized laboratory. All the vitreous samples from our patients were shipped as instructed ,,, to the immunopathology Section of the National Eye Institute in the United States, according to a research project with Dr. Chi-Chao Chan.
Cytological examination is considered the gold standard for diagnosis of intraocular lymphoma. , Cytology showed large atypical lymphoid cells with scant cytoplasm, pleomorphic nuclei, and prominent multiple nucleoli [Figure 3] that showed the classic phenotypic profile by immunohistochemistry.  Molecular analysis is currently gaining credit as a diagnostic tool, and it is currently debated if cytology is the most sensitive and specific technique for diagnosing intraocular lymphomas.  All our samples underwent microdissection and PCR analysis demonstrating IgH gene rearrangement. , In this study, all cases of intraocular B-cell lymphoma were identified by molecular analysis using primers FR2A, FR3A, and/or CDR3 that covered the CDR3 region of the IgH gene in B-cell lymphoma. 
Finally, in all seven cases, the IL-10 to IL-6 ratio was >1, being highly suggestive for VRL. ,
VRLs are severe diseases with a poor prognosis, and their incidence is increasing. They may resemble inflammatory eye diseases and may equivocally respond to systemic corticosteroids. Nevertheless, their clinical appearance - yellowish infiltrates at the retinal pigment epithelium level and vitreous haze - is quite characteristic [Figure 5], [Figure 6], [Figure 7], [Figure 8]. When tests for uveitis are not diagnostic in a patient with bilateral subretinal infiltrates, a VRL should be suspected. Because these malignancies have CNS tropism, lumbar puncture, and a brain MRI should be requested. If these last tests are negative, the presence of a VRL is still possible. Therefore, a diagnostic vitrectomy should be considered and followed by adequate cytological and molecular evaluation. Careful ophthalmic examination, appropriate consideration of past medical history, and a diagnostic vitrectomy timely performed could lead to the correct diagnosis of intraocular lymphoma in less than a month.
We wish to thank Ione Tamagnini PhD, Davide Niccoli PhD and Raffaella Aldigeri PhD for their contribution in laboratory tests and data management, respectively.
Financial support and sponsorship
Conflicts of interest
There are no conflicts of interest.
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