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OPHTHALMIC IMAGE
Year : 2020  |  Volume : 68  |  Issue : 10  |  Page : 2252

Corneal epithelial cells division assessed by scanning electron microscopy


Department of Corneal and Anterior Segment, Hospital Clinic of Barclona, Barcelona, Spain

Date of Web Publication23-Sep-2020

Correspondence Address:
Dr. Carlos Rocha-de-Lossada
Hospital Clinic of Barcelona, Barcelona
Spain
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Source of Support: None, Conflict of Interest: None


DOI: 10.4103/ijo.IJO_1214_20

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How to cite this article:
Rocha-de-Lossada C, Torras-Sanvicens J, Peraza-Nieves J. Corneal epithelial cells division assessed by scanning electron microscopy. Indian J Ophthalmol 2020;68:2252

How to cite this URL:
Rocha-de-Lossada C, Torras-Sanvicens J, Peraza-Nieves J. Corneal epithelial cells division assessed by scanning electron microscopy. Indian J Ophthalmol [serial online] 2020 [cited 2020 Oct 31];68:2252. Available from: https://www.ijo.in/text.asp?2020/68/10/2252/295662



Scanning electron microscopy (SEM) is a technique for obtaining high resolution images of biological and non-biological specimens. SEM has been used in the characterization of the surface of human corneo-conjunctival anatomy.[1]

The corneal epithelial cells divide in a rapid turnover to maintain the ocular surface homeostasis. Corneal epithelial cells are constantly replenished by limbal epithelial stem cells (LESC), after differentiation, they can proliferate by mitosis and migrate centripetally. Alterations to limbal environment can result in LESC dysfunction.[2]

Our aim is to show the ultrastructure by SEM of the corneal epithelial cells during mitotic division above the epithelial basal membrane. The cells are edematous in profile and intermediate in brightness, with multiple microprojections of different shapes, sizes, and numbers (microplicae and microvilli) [Figure 1]a and [Figure 1]b.
Figure 1 (a and b): Red arrow: Mitotic division. Yellow arrows: Epithelial basement membrane. Green arrows: Microvilli. Blue arrows: Lateral interdigitations

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Financial support and sponsorship

Nil.

Conflicts of interest

There are no conflicts of interest.



 
  References Top

1.
Versura P, Bonvicini F, Caramazza R, Laschi R. Scanning electron microscopy study of human cornea and conjunctiva in normal and various pathological conditions. Scan Electron Microsc. 1985;(Pt 4):1695-1708.  Back to cited text no. 1
    
2.
Zhao Y, Ma L. Systematic review and meta-analysis on transplantation of ex vivo cultivated limbal epithelial stem cell on amniotic membrane in limbal stem cell deficiency. Cornea 2015;34:592-600.  Back to cited text no. 2
    


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