|Year : 2020 | Volume
| Issue : 10 | Page : 2252
Corneal epithelial cells division assessed by scanning electron microscopy
Carlos Rocha-de-Lossada, Josep Torras-Sanvicens, Jorge Peraza-Nieves
Department of Corneal and Anterior Segment, Hospital Clinic of Barclona, Barcelona, Spain
|Date of Web Publication||23-Sep-2020|
Dr. Carlos Rocha-de-Lossada
Hospital Clinic of Barcelona, Barcelona
Source of Support: None, Conflict of Interest: None
|How to cite this article:|
Rocha-de-Lossada C, Torras-Sanvicens J, Peraza-Nieves J. Corneal epithelial cells division assessed by scanning electron microscopy. Indian J Ophthalmol 2020;68:2252
|How to cite this URL:|
Rocha-de-Lossada C, Torras-Sanvicens J, Peraza-Nieves J. Corneal epithelial cells division assessed by scanning electron microscopy. Indian J Ophthalmol [serial online] 2020 [cited 2020 Oct 31];68:2252. Available from: https://www.ijo.in/text.asp?2020/68/10/2252/295662
Scanning electron microscopy (SEM) is a technique for obtaining high resolution images of biological and non-biological specimens. SEM has been used in the characterization of the surface of human corneo-conjunctival anatomy.
The corneal epithelial cells divide in a rapid turnover to maintain the ocular surface homeostasis. Corneal epithelial cells are constantly replenished by limbal epithelial stem cells (LESC), after differentiation, they can proliferate by mitosis and migrate centripetally. Alterations to limbal environment can result in LESC dysfunction.
Our aim is to show the ultrastructure by SEM of the corneal epithelial cells during mitotic division above the epithelial basal membrane. The cells are edematous in profile and intermediate in brightness, with multiple microprojections of different shapes, sizes, and numbers (microplicae and microvilli) [Figure 1]a and [Figure 1]b.
|Figure 1 (a and b): Red arrow: Mitotic division. Yellow arrows: Epithelial basement membrane. Green arrows: Microvilli. Blue arrows: Lateral interdigitations|
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Conflicts of interest
There are no conflicts of interest.
| References|| |
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cultivated limbal epithelial stem cell on amniotic membrane in limbal stem cell deficiency. Cornea 2015;34:592-600.