|Year : 2020 | Volume
| Issue : 11 | Page : 2439-2444
Hypochlorous acid antiseptic washout improves patient comfort after intravitreal injection: A patient reported outcomes study
Anthony Fam, Paul T Finger, Ankit S Tomar, Gaurav Garg, Kimberly J Chin
The New York Eye Cancer Center, New York, New York, USA
|Date of Submission||18-Jun-2020|
|Date of Acceptance||21-Sep-2020|
|Date of Web Publication||26-Oct-2020|
Paul T Finger
MD, The New York Eye Cancer Center, Fifth Floor, 115 East 61st Street, New York, NY 10065
Source of Support: None, Conflict of Interest: None
Purpose: Current ocular antiseptic practice for intravitreal injection (IVI) employs 5% povidone–iodine (Betadine®) drops which frequently cause ocular discomfort and prolonged irritation. In an effort to improve comfort while maintaining efficacy, we studied a hypochlorous acid (HOCL 0.01%) spray washout prior to injection. Methods: Patients had received a minimum of 3 IVIs prepared with Betadine®antisepsis prior to entry in this study. Their subsequent IVIs were prepared with Betadine®followed by HOCL 0.01% washout. Facets of comfort were measured by a Likert-scaled questionnaire to compare their experiences after IVI. Results: Thirty-seven participants were enrolled. Addition of HOCL 0.01% spray after Betadine®reduced the duration of discomfort (P = 0.001) and need for artificial tears postinjection (P = 0.003). It improved their reported quality of life (P = 0.04) and sleep (P = 0.01). There were neither HOCL-related side effects nor endophthalmitis during this study. Conclusion: Topical HOCL 0.01% spray after topical Betadine®antisepsis significantly improved patient comfort following IVIs.
Keywords: Antisepsis, Betadine®, comfort, HOCL, hypochlorous acid, intravitreal, PRO, prophylaxis, quality of life
|How to cite this article:|
Fam A, Finger PT, Tomar AS, Garg G, Chin KJ. Hypochlorous acid antiseptic washout improves patient comfort after intravitreal injection: A patient reported outcomes study. Indian J Ophthalmol 2020;68:2439-44
|How to cite this URL:|
Fam A, Finger PT, Tomar AS, Garg G, Chin KJ. Hypochlorous acid antiseptic washout improves patient comfort after intravitreal injection: A patient reported outcomes study. Indian J Ophthalmol [serial online] 2020 [cited 2020 Nov 28];68:2439-44. Available from: https://www.ijo.in/text.asp?2020/68/11/2439/299040
Intraocular vasculopathies such as age-related macular degeneration, diabetic macular edema, retinal vein occlusions, radiation retinopathy, radiation optic neuropathy, and other such pathologies utilize intravitreal antivascular endothelial growth factor and steroids in their treatment.,,,, Thus, intravitreal injections (IVIs) have become the most frequently performed procedure in ophthalmology. One of the most feared complications of IVI is endophthalmitis because it risks loss of vision, loss of eye, and mental health. Studies have revealed the likelihood of endophthalmitis to be 0.019–0.09% per IVI. To prevent this complication, disinfection of the conjunctival surface is typically performed prior to injection.
Povidone–iodine (Betadine®, Alcon, Fort Worth, Texas, USA) (Betadine®) is the most commonly used antiseptic solution prior to IVI. Conjunctival surface disinfection guidelines recommend 5–10% Betadine®. Studies have supported this practice, showing that the risk of endophthalmitis is higher in patients who are not pretreated. For prophylaxis, one drop of Betadine® 5% aqueous solution has been applied to the conjunctival surface 30 seconds prior to IVI through the pars plana. However, many studies report that patients can experience allergic reactions, pain, edema, and significant discomfort after Betadine®. Patients have reported that the most uncomfortable part of the IVI procedure is the Betadine® antisepsis and that anesthetics are not sufficient in preventing this discomfort. Since IVIs have been administered routinely on a monthly basis, this poses a considerable problem for patients and clinicians, as pain has been shown to negatively impact treatment compliance.
Hypochlorous acid (HOCL) 0.01% (Avenova®, NovaBay Pharmaceuticals, Inc., Emeryville, California, USA) is a potent oxidizing agent effective against a wide spectrum of organisms, including the most common bacteria implicated in postinjection endophthalmitis [Table 1].,,, This form of HOCL 0.01% is commercially formulated free of sodium hypochlorite and at a pH of 6.5-7.0. It simultaneously possesses anti-inflammatory properties and has been used safely cutaneously on the eyelids as an antimicrobial and in wound healing, making it a viable alternative or addition to the current standard of care for IVI prophylaxis, Betadine®.,, Herein, we present a patient reported outcomes (PRO) study that seeks to evaluate comfort after Betadine® 5% antisepsis, followed by HOCL 0.01% washout prior to IVI.
| Methods|| |
Institutional Review Board (IRB) approval for this prospective cohort PRO analysis was obtained from the The New York Eye Cancer Center IRB and Ethics committees. This study was conducted at a single practice between November 2019 and January 2020. Every consecutive patient since November 2019 receiving IVIs at this single center was eligible for enrollment. Inclusion criterion was that every patient must have received ocular surface antiseptic preparation for IVIs with Betadine® 5% alone for more than three prior injections. Exclusion criteria included patients below the age of 18 and patients unable to comprehend or answer the questions asked in the questionnaire. These patients were then eligible to begin receiving antiseptic preparation with 1 drop of Betadine® 5%, followed by six or more sprays of epibulbar HOCL 0.01% prior to the injection. This number of sprays was utilized so that the brown of the Betadine no longer appeared visible on the ocular surface. This ensured that a majority of the Betadine was effectively washed out by the HOCL 0.01%. PROs comparing each patient's experiences were then collected following three IVIs, which utilized the new antiseptic technique. All subjects therefore received a minimum total of 6 IVIs: 3 with Betadine alone, and 3 with Betadine followed by HOCL 0.01%. They then answered the questionnaire and provided written informed consent for participation, use of Avenova®, and publication of their results.
Comparative antibiotic coverage
Betadine® 5% and HOCL 0.01% are both characterized by their broad-spectrum antibacterial, antifungal, and antiviral activity.,,,,, However, most commonly used prior to ophthalmic surgeries and IVI, Betadine® 5% was selected for its proven efficacy in significantly reducing the rate of endophthalmitis in patients receiving IVI. In contrast, HOCL 0.01% has been used to treat blepharitis and meibomian gland dysfunction and thus dry eye. However, in our comparative review of the literature, HOCL 0.01% was found to offer even wider antibiotic coverage for actinobacteria replace with for actinobacteria, bacterial spores, and viruses [Table 1].
The anesthetic protocol used for all patients included: one drop of viscous ophthalmic lidocaine gel (Akten, Akorn, Lake Forest, Illinois, USA), followed again by a second drop after 4 min. After an additional 4 min, all patients received one drop of Betadine® 5% preparation onto the inferior fornix 30 seconds prior to HOCL 0.01%. Then, the eyelids are manually opened and a minimum of six sprays of HOCL 0.01% was used to prepare the eye and eyelids. A single physician administered all drops, sprays, and injections. IVIs utilized a 1 cc syringe and a 30-gauge needle. Manual eyelid retraction allowed access to the inferior bulbar conjunctiva. There, angled self-sealing transscleral injections were placed through the pars plana into the vitreous.
Patient reported outcomes measures
A unique Likert-style patient survey was designed based on the Ocular Surface Disease Index patient questionnaire for dry eyes. However, our PRO survey measured ocular surface discomfort and quality of life in association with either Betadine® only or Betadine® followed by HOCL 0.01%. The questionnaire was comprised of 10 items to evaluate five measures of patient comfort for each of the two arms of the study [Table 2]. The five measures included: duration of discomfort after injection, the frequency of use of pain medication, the frequency of use of postinjection artificial tears, the frequency of disturbance of quality of life and vision, and the frequency of sleep disturbance. There are four options for answering each question. This format was based on a Likert-scale questionnaire with scores of 1–4 given to answers “Never,” “Once in a While,” “Often,” and “Always,” respectively. The questions addressing duration of discomfort after injection were also in Likert-scale format, with scores of 1–4 given to answers “Seconds,” “Minutes,” “Hours,” and “Days,” respectively. A four-point Likert scale was used as there is no neutral response that a patient could report on an even scaling. Furthermore, given the patient population and the average age at which they completed the survey, a four-point scale was determined to be easier for comprehension purposes and faster to complete. The reliability of the questionnaire and its answers was determined using Cronbach's Alpha. Finally, background information such as age, sex, race, past medical history, and pre-existing ocular surface disease were collected separately.
Both the questionnaire and our medical staff informed patients that their participation was voluntary, that they can withdraw their responses from the study if they felt uncomfortable being included. Patients were also informed that their responses will be kept confidential and their personal health information would be both encrypted and password protected. In addition, patients were randomly assigned a number which was linked to each of their names, which corresponded with the patient's questionnaire submission. Additionally, no patient identifiers are present in any of the remainder of the data collected.
The Cronbach's Alpha test was employed to determine the internal consistency between the scaled items in the test. This determines if the questionnaire employed is a reliable test. A value greater than 0.700 was considered to indicate high test reliability. Wilcoxon's signed-rank test was performed to estimate the change in comfort scores before and after the addition of HOCL 0.01% to the conventional Betadine® antiseptic prophylaxis. A P-value less than 0.05 was considered statistically significant. The data were analyzed using commercially available software (SPSS version 18.0; SPSS, Inc., Chicago, IL).
| Results|| |
Thirty-nine patients from a single clinical center enrolled in the study. Two subjects did not participate due to an inability to comprehend and answer the questions, resulting in a total of 37 study participants. There were no patients less than 18 years of age or pregnant females. Of these patients, 15 were female (40.5%). The average age of the cohort was 62.4 years (range 29–83 years). Thirty-one of the patients (83.8%) identified as “Caucasian,” 5 (13.5%) identified as “African American or black,” and 1 (2.7%) identified as “other.” Underlying illnesses in the cohort include hypertension (24/37, 64.9%), diabetes (5/37, 13.5%), and prior cardiac illness (5/37, 13.5%).
Furthermore, the Cronbach's Alpha scores for measuring the employed questionnaire's reliability showed scores of 0.723 for the Betadine alone question set, and 0.704 for the Betadine with Avenova question set. These numbers indicate high reliability of the questionnaire administered in this study. Finally, a Wilcoxon signed-rank test was performed to compare and analyze the five measures of patient comfort between both antiseptic preparations [Table 3].
|Table 3: Results of Wilcoxon signed.rank test in evaluation of questionnaire|
Click here to view
Duration of patient comfort
For the question assessing the duration of patient comfort, a small number of participants (5/37, 13.5%) responded that they experienced discomfort/ irritation for seconds after the procedure when Betadine® 5% was used alone. In contrast, the addition of HOCL 0.01% caused a large shift, where the majority (20/37, 54.1%) responded that they experienced discomfort/irritation for only seconds after the procedure. The shift from Betadine® alone to Betadine® followed by HOCL 0.01% resulted in a statistically significant reduction in duration of postinjection discomfort (P = 0.001).
Use of pain medication
For the question assessing patients' use of pain medication following IVIs, the majority (26/37, 70.3%) responded that they never used pain medication after the use of Betadine® 5% alone. However, with HOCL 0.01%, the majority (28/37, 75.5%) responded that they never used pain medication after the procedure. The change in use of pain medication was not statistically significant (P = 0.20).
Use of artificial tears
For the question assessing patients' use of artificial tears following IVIs, a large number of patients (18/37, 48.6%) responded that they require artificial tears following Betadine® 5% alone. With the addition of HOCL 0.01% spray washout, a majority of patients (27/37, 73%) responded that they did not require artificial tears. The decrease in usage of artificial tears was found to be statistically significant (P = 0.003).
Quality of life
For the question assessing patients' quality of life following IVIs, the majority (20/37, 54.1%) responded that they experienced a decrease in their quality of life after Betadine® 5% alone. In contrast, with the addition of HOCL 0.01% spray washout, a larger majority of patients (24/37, 64.9%) responded that they never experienced a change in their quality of life after the procedure. The addition of HOCL 0.01% spray washout resulted in a statistically significant increase in patients' reported quality of life (P = 0.04).
Quality of sleep
For the question assessing patients' quality of sleep following IVIs, a large number of patients (15/37, 40.5%) responded that they experienced a change in quality of sleep following Betadine® 5% prophylaxis. However, with the addition of HOCL 0.01% spray washout, a majority of patients (32/37, 86.5%) responded that they never experienced a change in their quality of sleep after their injection. This study found that the shift from Betadine® 5% alone to Betadine® followed by HOCL 0.01%. spray washout resulted in a statistically significant improvement in patients' quality of sleep (P = 0.01).
| Discussion|| |
Herein, we present the first PRO study assessing patient comfort with the use of HOCL 0.01% spray washout antisepsis following Betadine® prophylaxis for IVI. The questionnaire developed and employed in this study evaluated five distinct aspects of patient comfort. We found that the use of HOCL 0.01% significantly reduced patients' experience of discomfort following the procedure. Specifically, the duration of discomfort was reduced from hours to mere seconds, the need for artificial tears was halved, and a significant improvement in the quality of life and sleep were reported.
Traditionally, Betadine® has been used for antiseptic prophylaxis and has demonstrated efficacy in diminishing the incidence of postinjection ocular infections., The microbial spectrum covered by Betadine® 5% provides strong coverage against a majority of the microorganisms implicated in endophthalmitis.,,, However, because of the associated discomfort with ocular surface Betadine®, patients may be disinclined towards frequently administered IVIs, which may result in poor follow-up in a large number of patients. Betadine® causes this discomfort due to its composition and its associated chemical constituents, which are known to be ocular irritants. Prior studies have attempted to resolve the discomfort associated with Betadine® by utilizing dilutions of Betadine®, as well as by utilizing saline irrigation postinjection. In these studies, Betadine® diluted to 2.5, 1.25, and 0.5% provided improvement in postinjection patient comfort, but did not consistently demonstrate a protective effect in-vivo. In contrast, postinjection saline irrigation has been associated with significantly fewer corneal epithelial defects but did not significantly improve patient discomfort.
HOCL 0.01% has been shown in a number of studies to have comparable antimicrobial activity to Betadine and may cover additional viruses and bacterial species more effectively [Table 1].,,,,,,,,,, A number of studies have demonstrated that HOCL is a naturally occurring anti-inflammatory agent that functions well as an antiseptic preparation., Gold et al. found that HOCL 0.01% significantly reduced inflammation and was effective in killing >99.9% of tested microbes. Likewise, Ngo et al. evaluated the comfort levels of several eyelid cleansing products in the treatment of blepharitis associated with Demodex folliculorum and found HOCL 0.01% to have the highest levels of patient comfort following treatment. This may in part be explained by the ability of HOCL to mediate inflammatory cytokines, such as reducing the activity of leukotriene B4, histamine and interleukin-2, all of which are involved in the development of pruritus and irritation. Finally, when HOCL is employed as a washout, it serves to remove residual irritant particles of Betadine, without diluting its antimicrobial effect, since it is a comparable antimicrobial.
The results of the present study concurred with the above literature in that HOCL 0.01% spray washout demonstrated a significant reduction in discomfort while increasing the patients' quality of life. This study hypothesizes, but did not prove, that the use of HOCL 0.01% spray following Betadine® not only reduced the irritation following IVIs but did so without diminishing the antimicrobial effect [Table 1]. Clearly, our study only addresses HOCL's ability to improve patient comfort after IVIs. This study did not show that HOCL 0.01% can replace Betadine 5% for antibiotic prophylaxis. That would best be proven utilizing a randomized control trial comparing HOCL 0.01% spray to Betadine®, which is beyond the scope of this study.
Our study provided medical evidence that HOCL 0.01% spray washout improved patient comfort after Betadine 5% prophylaxis. Our patients responded to a 10-question survey to compare their past to their current experience, making recall bias a potential weakness of the study. However, recall bias would not change the overall consensus that patients had a significant preference for the use of HOCL 0.01% spray after Betadine® versus Betadine® alone. Another weakness of this study is its sample size. More patients were not included as this was a pilot study, and a larger sample size is being recruited, given the positive findings exhibited in the present study. Despite this, the results showed a statistically significant patient preference for the inclusion of HOCL 0.01% spray and improved the quality of life, even in the relatively small cohort. Future studies will be needed to evaluate the efficacy and patient satisfaction of HOCL 0.01% spray as a monotherapy prophylaxis. Finally, the use of Betadine® before the HOCL 0.01% spray may have reduced the comfort that might be found after monotherapy with HOCL 0.01% spray. Even with this limitation, the addition of HOCL 0.01% spray still reduced irritation and increased the quality of life as compared to Betadine® alone.
The strengths to this study are that it is the first to evaluate HOCL 0.01% spray as a means to improve comfort related to ocular IVI prophylaxis. All of the patients experienced both arms of the study. They were therefore able to directly compare their experience with Betadine® alone to their experience with Betadine® with HOCL 0.01% spray. This thereby eliminated any confounding factors inherent to subjective pain reporting, since pain would expectedly have high interpatient variability. Additionally, given its demonstrated prophylactic efficacy, the standard 5% Betadine® was employed throughout this study. The HOCL 0.01% spray washout was a potent antimicrobial which ensured that, compared to saline washout, the antibiotic effect was not diminished. This is an additional strength over the use of saline or saline-lubricant postinjection, which may dilute the antibiotic effect of Betadine®.
| Conclusion|| |
This study suggests that the use of HOCL 0.01% spray after Betadine® 5% for IVI prophylaxis will offer improved patient comfort as a rinse for Betadine®. The known spectrum of antiseptic coverage provided by HOCL 0.01% spray was equal to or better than Betadine®, and affects the majority of the pathogens commonly implicated in endophthalmitis. This research reports on the novel use of HOCL 0.01% as an ocular surface anti-septic. Clearly, a larger study will be required to evaluate its safety profile at preventing endophthalmitis when used as prophylaxis prior to IVIs.
Supported by The Eye Cancer Foundation, Inc., New York, New York (http://eyecancercure.com). The sponsor or funding organization had no role in the design or conduct of this research. Dr Finger holds United States of America Patent # W02008060491A2 “Anti-VEGF treatment for radiation induced vasculopathy.” However, no conflicting relationship exists for any author.
Financial support and sponsorship
Conflicts of interest
There are no conflicts of interest.
| References|| |
Lanzetta P, Mitchell P, Wolf S, Veritti D. Different antivascular endothelial growth factor treatments and regimens and their outcomes in neovascular age-related macular degeneration: A literature review. Br J Ophthalmol 2013;97:1497-507.
Elman MJ, Ayala A, Bressler NM, Browning D, Flaxel CJ, Glassman AR, et al
. Intravitreal ranibizumab for diabetic macular edema with prompt versus deferred laser treatment: 5-year randomized trial results. Ophthalmology 2015;122:375-81.
Sun Y, Qu Y. Comparison of intravitreal bevacizumab with intravitreal triamcinolone acetonide for treatment of cystoid macular edema secondary to retinal vein occlusion: A Meta-analysis. Int J Ophthalmol 2015;8:1234-9.
Finger PT. Radiation retinopathy is treatable with anti-vascular endothelial growth factor bevacizumab (Avastin). Int J Radiat Oncol Biol Phys 2008;70:974-7.
Finger PT. Anti-VEGF bevacizumab (Avastin) for radiation optic neuropathy. Am J Ophthalmol 2007;143:335-8.
Lau PE, Jenkins KS, Layton CJ. Current evidence for the prevention of endophthalmitis in anti-VEGF intravitreal injections. J Ophthalmol 2018;2018:8567912.
Haddock LJ, Ramsey DJ, Young LH. Complications of subspecialty ophthalmic care: Endophthalmitis after intravitreal injections of anti-vascular endothelial growth factor medications. Semin Ophthalmol 2014;29:257-62.
Avery RL, Bakri SJ, Blumenkranz MS, Brucker AJ, Cunningham ET Jr, D'Amico DJ, et al
. Intravitreal injection technique and monitoring: Updated guidelines of an expert panel. Retina 2014;34(Suppl 12):S1-18.
Bhavsar AR, Glassman AR, Stockdale CR, Jampol LM; Diabetic Retinopathy Clinical Research N. Elimination of topical antibiotics for intravitreous injections and the importance of using povidone-iodine: Update from the diabetic retinopathy clinical research network. JAMA Ophthalmol 2016;134:1181-3.
Ridder WH 3rd, Oquindo C, Dhamdhere K, Burke J. Effect of povidone iodine 5% on the cornea, vision, and subjective comfort. Optom Vis Sci 2017;94:732-41.
Reynolds MM, Greenwood-Quaintance KE, Patel R, Pulido JS. Selected antimicrobial activity of topical ophthalmic anesthetics. Transl Vis Sci Technol 2016;5:2.
Vaze A, Fraser-Bell S, Gillies M. Reasons for discontinuation of intravitreal vascular endothelial growth factor inhibitors in neovascular age-related macular degeneration. Retina 2014;34:1774-8.
Durand ML. Endophthalmitis. Clin Microbiol Infect 2013;19:227-34.
Stroman DW, Mintun K, Epstein AB, Brimer CM, Patel CR, Branch JD, et al
. Reduction in bacterial load using hypochlorous acid hygiene solution on ocular skin. Clin Ophthalmol 2017;11:707-14.
Romanowski EG, Stella NA, Yates KA, Brothers KM, Kowalski RP, Shanks RMQ. In vitro
evaluation of a hypochlorous acid hygiene solution on established biofilms. Eye Contact Lens 2018;44(Suppl 2):S187-91.
Anagnostopoulos AG, Rong A, Miller D, Tran AQ, Head T, Lee MC, et al
. 0.01% hypochlorous acid as an alternative skin antiseptic: An in vitro
comparison. Dermatol Surg 2018;44:1489-93.
Ngo W, Jones L, Bitton E. Short-term comfort responses associated with the use of eyelid cleansing products to manage demodex folliculorum. Eye Contact Lens 2018;44(Suppl 2):S87-92.
Gold MH, Andriessen A, Bhatia AC, Bitter P
Jr, Chilukuri S, Cohen JL, et al
. Topical stabilized hypochlorous acid: The future gold standard for wound care and scar management in dermatologic and plastic surgery procedures. J Cosmet Dermatol 2020;19:270-7.
Wang L, Bassiri M, Najafi R, Najafi K, Yang J, Khosrovi B, et al
. Hypochlorous acid as a potential wound care agent: Part I. Stabilized hypochlorous acid: A component of the inorganic armamentarium of innate immunity. J Burns Wounds 2007;6:e5.
Kawana R, Kitamura T, Nakagomi O, Matsumoto I, Arita M, Yoshihara N, et al
. Inactivation of human viruses by povidone-iodine in comparison with other antiseptics. Dermatology 1997;195(Suppl 2):29-35.
Koerner JC, George MJ, Meyer DR, Rosco MG, Habib MM. Povidone-iodine concentration and dosing in cataract surgery. Surv Ophthalmol 2018;63:862-8.
Lachapelle J-M, Castel O, Casado AF, Leroy B, Micali G, Tennstedt D, et al
. Antiseptics in the era of bacterial resistance: A focus on povidone iodine. Clin Pract 2013;10:579-92.
Mehta MC, Finger PT. Angled transscleral intravitreal injection: A crossover study. Eur J Ophthalmol 2015;25:173-6.
Tavakol M, Dennick R. Making sense of Cronbach's alpha. Int J Med Educ 2011;2:53-5.
Koerner JC, George MJ, Kissam EA, Rosco MG. Povidone-iodine concentration and in vitro
killing time of bacterial corneal ulcer isolates. Digit J Ophthalmol 2018;24:24-6.
Ghosh YK, Ahluwalia H, Beamer J. Povidone-iodine antisepsis before ophthalmic surgery. Anaesthesia 2006;61:1128-9.
Peden MC, Hammer ME, Suner IJ. Dilute povidone-iodine prophylaxis maintains safety while improving patient comfort after intravitreal injections. Retina 2019;39:2219-24.
Jandorf S, Krogh Nielsen M, Sorensen TL. Irrigating the eye after intravitreal injection reduces epithelial damage but not patient discomfort. Acta Ophthalmol 2019;97:e670-1.
Chapman I, Hsu JTS, Stankiewicz K, Bhatia AC. Use of hypochlorous acid as a preoperative antiseptic before placement of dermal fillers: An alternative to the standard options. Dermatol Surg 2018;44:597-9.
Sakarya S, Gunay N, Karakulak M, Ozturk B, Ertugrul B. Hypochlorous acid: An ideal wound care agent with powerful microbicidal, antibiofilm, and wound healing potency. Wounds 2014;26:342-50.
Pelgrift RY, Friedman AJ. Topical hypochlorous acid (HOCl) as a potential treatment of pruritus. Curr Dermatol Rep 2013;2:181-90.
Berkelman RL, Holland BW, Anderson RL. Increased bactericidal activity of dilute preparations of povidone-iodine solutions. J Clin Microbiol 1982;15:635-9.
Apt L, Isenberg S, Yoshimori R, Paez JH. Chemical preparation of the eye in ophthalmic surgery. III. Effect of povidone-iodine on the conjunctiva. Arch Ophthalmol 1984;102:728-9.
Grzybowski A, Brona P. Povidone-iodine is still a premium antiseptic measure in ocular surgery. Acta Ophthalmol 2017;95:e253-4.
Grzybowski A, Kanclerz P, Myers WG. The use of povidone-iodine in ophthalmology. Curr Opin Ophthalmol 2018;29:19-32.
Kim HJ, Lee JG, Kang JW, Cho HJ, Kim HS, Byeon HK, et al
. Effects of a low concentration hypochlorous Acid nasal irrigation solution on bacteria, fungi, and virus. Laryngoscope 2008;118:1862-7.
Wigginton KR, Pecson BM, Sigstam T, Bosshard F, Kohn T. Virus inactivation mechanisms: Impact of disinfectants on virus function and structural integrity. Environ Sci Technol 2012;46:12069-78.
Finger PT, Fam A, Tomar A, Garg G, Chin KJ. COVID-19 prophylaxis in ophthalmology. Indian J Ophthalmol 2020;68:2062-3.
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[Table 1], [Table 2], [Table 3]