AU - Jain, Nayan AU - Rawal, U TI - Influence of UV-irradiation on enzymes in mouse ocular lens: in vitro studies PT - ORIG DP - 1999 Jan 1 TA - Indian Journal of Ophthalmology PG - 25-29 VI - 47 IP - 1 4099- https://journals.lww.com/ijo/pages/default.aspx/article.asp?issn=0301-4738;year=1999;volume=47;issue=1;spage=25;epage=29;aulast=Jain;type=0 4100- https://journals.lww.com/ijo/pages/default.aspx/article.asp?issn=0301-4738;year=1999;volume=47;issue=1;spage=25;epage=29;aulast=Jain AB - Purpose: In vitro study of the enzymes involved in aerobic, anaerobic and hexose monophosphate shunt in ultraviolet radiation exposed mice lenses. Method: Of the selected enzymes, lactic dehydrogenase (LDH) was representative of anaerobic glucose oxidation, succinic dehydrogenase (SDH) of the aerobic oxidation, and Glucose-6-phosphate dehydrogenase (G-6-PDH) of the Hexose Monophosphate (HMP) shunt. Other enzymes studied were ATPase and glutathione reductase (GR). Results: Experiments with mice lenses in vitro showed that transparent lens became opaque following UV-irradiation at 360 nm. Opacification of the lens was accompanied by a change in enzyme activities for energy metabolism. Conclusion: These changes were progressive in a manner analogous to sequential morphological changes, which would be crucial in maintaining lens transparency.