RT - Journal TY - JOUR A1 - Simsek, Cem A1 - Gokmen, Onur T1 - The effects of vıtal dyes on mechanıcal propertıes of the human anterıor lens capsule YR - 2020/1/1 JF - Indian Journal of Ophthalmology JO - Indian J Ophthalmol SP - 66 OP - 70 VO - 68 IS - 1 UL - https://journals.lww.com/ijo/pages/default.aspx/article.asp?issn=0301-4738;year=2020;volume=68;issue=1;spage=66;epage=70;aulast=Simsek;t=5 DO - 10.4103/ijo.IJO_285_19 N2 - Purpose: The purpose of this study was to evaluate the mechanical effects of vital dyes on the anterior lens capsule via the nanoindentation method. Methods: Twenty anterior lens capsules of 20 different patients were dissected into four equal fragments. Each fragment was stained separately with dyes for intraocular surgeries, such as trypan blue 0.06% (TB), brilliant blue 0.025% (BB), and indocyanine green 0.05% (ICG), for 1 min. The remaining fragment was assessed as an untreated control group. The alterations on the mechanical characteristics of the anterior lens capsule were evaluated using a nanoindentation testing device with Oliver–Pharr and Martens hardness methods. Results: The mean values of elasticity were 7.842 ± 0.55 GPa for capsules fragments stained with TB (P < 0.05), 8.407 ± 0.82 GPa for capsules fragments stained with BB (P < 0.05), 8.557 ± 0.60 GPa for ICG (P < 0.05), and 6.09 ± 0.57 GPa for the untreated control group. The mean values of stiffness were 299.7 ± 47 MPa for TB (P < 0.05), 317.9 ± 34 MPa for BB (P < 0.05), 331.8 ± 48 MPa for ICG (P < 0.05), and 229.85 ± 44 MPa for the untreated control group. The elasticity of the capsules statistically decreased in comparison to the control group, and the capsule stiffness showed a statistically significant increase in comparison to the untreated controls. Conclusion: The mechanical characteristics of the human anterior lens capsule were affected in association with the alterations in the elasticity and stiffness properties of the capsule as a result of exposure to three different dyes. ER -