ORIGINAL ARTICLE
Year : 1998 | Volume
: 46 | Issue : 1 | Page : 37--40
Effects of viscoelastic ophthalmic solutions on cell cultures
HN Madhavan, S Roy Vision Research Foundation, Sankara Nethralaya, Chennai, India
Correspondence Address:
H N Madhavan Vision Research Foundation, Sankara Nethralaya, Chennai India
The development of mild but significant inflammation probably attributable to viscoelastic ophthalmic solutions in cataract surgery was recently brought to the notice of the authors, and hence a study of the effects of these solutions available in India, on cell cultures was undertaken. We studied the effects of 6 viscoelastic ophthalmic solutions (2 sodium hyaluronate designated as A and B, and 4 hydroxypropylmethylcellulose designated as C, D, E and F) on HeLa, Vero and BHK-21 cell lines in tissue culture microtitre plates using undiluted, 1:10 and 1:100 dilutions of the solutions, and in cover slip cultures using undiluted solutions. Phase contrast microscopic examination of the solutions was also done to determine the presence of floating particles. The products D and F produced cytotoxic changes in HeLa cell line and these products also showed the presence of floating particles under phase contrast microscopy. Other products did not have any adverse effects on the cell lines nor did they show floating particles. The viscoelastic ophthalmic pharmaceutical products designated D and F have cytotoxic effects on HeLa cell line which appears to be a useful cell line for testing these products for their toxicity. The presence of particulate materials in products D and F indicates that the methods used for purification of the solution are not effective.
How to cite this article:
Madhavan H N, Roy S. Effects of viscoelastic ophthalmic solutions on cell cultures.Indian J Ophthalmol 1998;46:37-40
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How to cite this URL:
Madhavan H N, Roy S. Effects of viscoelastic ophthalmic solutions on cell cultures. Indian J Ophthalmol [serial online] 1998 [cited 2023 Mar 27 ];46:37-40
Available from: https://journals.lww.com/ijo/pages/default.aspx/article.asp?issn=0301-4738;year=1998;volume=46;issue=1;spage=37;epage=40;aulast=Madhavan;type=0 |
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