Indian Journal of Ophthalmology

ORIGINAL ARTICLE
Year
: 1999  |  Volume : 47  |  Issue : 1  |  Page : 25--29

Influence of UV-irradiation on enzymes in mouse ocular lens: in vitro studies


Nayan K Jain, UM Rawal 
 Department of Zoology, School of Sciences, Gujarat University, Ahmedabad, India

Correspondence Address:
Nayan K Jain
Dept. of Zoology, School of Sciences, Gujarat University, Ahmedabad
India

Purpose: In vitro study of the enzymes involved in aerobic, anaerobic and hexose monophosphate shunt in ultraviolet radiation exposed mice lenses. Method: Of the selected enzymes, lactic dehydrogenase (LDH) was representative of anaerobic glucose oxidation, succinic dehydrogenase (SDH) of the aerobic oxidation, and Glucose-6-phosphate dehydrogenase (G-6-PDH) of the Hexose Monophosphate (HMP) shunt. Other enzymes studied were ATPase and glutathione reductase (GR). Results: Experiments with mice lenses in vitro showed that transparent lens became opaque following UV-irradiation at 360 nm. Opacification of the lens was accompanied by a change in enzyme activities for energy metabolism. Conclusion: These changes were progressive in a manner analogous to sequential morphological changes, which would be crucial in maintaining lens transparency.


How to cite this article:
Jain NK, Rawal U M. Influence of UV-irradiation on enzymes in mouse ocular lens: in vitro studies.Indian J Ophthalmol 1999;47:25-29


How to cite this URL:
Jain NK, Rawal U M. Influence of UV-irradiation on enzymes in mouse ocular lens: in vitro studies. Indian J Ophthalmol [serial online] 1999 [cited 2022 Dec 2 ];47:25-29
Available from: https://www.ijo.in/article.asp?issn=0301-4738;year=1999;volume=47;issue=1;spage=25;epage=29;aulast=Jain;type=0