Indian Journal of Ophthalmology

ORIGINAL ARTICLE
Year
: 2011  |  Volume : 59  |  Issue : 1  |  Page : 5--8

Retinal nitric oxide and malonyldialdehyde levels following photodynamic therapy


Peykan Turkcuoglu1, Cem Ozturkmen2, Nevin Ilhan3, Julide Kurt1, Orhan Aydemir1, Ulku Celiker1, Mohamed A Ibrahim4, Aymen Rashid4 
1 Department of Ophthalmology, Firat University School of Medicine, Elazig, Turkey
2 Department of Ophthalmology, Goznur Eye Hospital, Gaziantep, Turkey
3 Department of Biochemistry, Firat University School of Medicine, Elazig, Turkey
4 Department of Ophthalmology, Wilmer Eye Institute, Johns Hopkins University School of Medicine, Baltimore, Maryland, USA

Correspondence Address:
Peykan Turkcuoglu
Inonu University School of Medicine, Department of Ophthalmology, Malatya
Turkey

Background: To determine the retinal nitric oxide (NO) and malonyldialdehyde (MDA) levels following photodynamic therapy (PDT). Materials and Methods: Seven Dutch-belted rabbits received dextrose, while seven others received 2 mg/kg verteporfin infusion over a period of 15 minutes in a dim-lit room. Irradiation to a 1.5 mm diameter intact chorioretinal area in the right eye of verteporfin-infused rabbits, was started 5 minutes after the end of infusion. Three groups were control (dextrose infusion), infusion with verteporfin (left eyes were not irradiated), and irradiation after verteporfin injection (right eyes were irradiated). On the fifth day of the experiment, the eyes were enucleated. The retinas were subsequently frozen and homogenized. Nitrite, a stable end-product of NO and MDA, was measured using the spectrophotometer. Protein concentrations were measured by the Lowry method. Tissue NO and MDA levels were expressed as μmol/gprt and nmol/mgprt, respectively. Results: The mean retinal NO and MDA levels of the control, infusion, and irradiation groups were 24.67 ± 6.66, 0.11 ± 0.02; 45.90 ± 15.52, 0.21 ± 0.09; and 84.43 ± 14.96 μmol/gprt, 0.58 ± 0.14 nmol/mgprt, respectively. The mean retinal NO levels were significantly elevated in the infusion and irradiation groups compared with the control group ( P :0.004; P :0.001). The mean retinal MDA levels were significantly elevated in the infusion and irradiation groups compared to the control one ( P :0.026; P :0.001). Also the mean retinal NO and MDA levels in the irradiation group were found to be significantly higher than the infusion group ( P :0.018; P :0.018). Conclusion: Not only PDT, but also verteporfin infusion alone resulted in NO and MDA level increments in the retina, which might be toxic.


How to cite this article:
Turkcuoglu P, Ozturkmen C, Ilhan N, Kurt J, Aydemir O, Celiker U, Ibrahim MA, Rashid A. Retinal nitric oxide and malonyldialdehyde levels following photodynamic therapy.Indian J Ophthalmol 2011;59:5-8


How to cite this URL:
Turkcuoglu P, Ozturkmen C, Ilhan N, Kurt J, Aydemir O, Celiker U, Ibrahim MA, Rashid A. Retinal nitric oxide and malonyldialdehyde levels following photodynamic therapy. Indian J Ophthalmol [serial online] 2011 [cited 2024 Mar 28 ];59:5-8
Available from: https://journals.lww.com/ijo/pages/default.aspx/article.asp?issn=0301-4738;year=2011;volume=59;issue=1;spage=5;epage=8;aulast=Turkcuoglu;type=0