Indian Journal of Ophthalmology

ORIGINAL ARTICLE
Year
: 2016  |  Volume : 64  |  Issue : 10  |  Page : 702--709

Recombinant interferon alpha 2b for ocular surface squamous neoplasia: An efficient and cost-effective treatment modality in Asian Indian patients


Swathi Kaliki, Swati Singh, Sadiya Iram, Dharani Tripuraneni 
 Ocular Oncology Services, The Operation Eyesight Universal Institute for Eye Cancer, L. V. Prasad Eye Institute, Hyderabad, Telangana, India

Correspondence Address:
Swathi Kaliki
The Operation Eyesight Universal Institute for Eye Cancer, L. V. Prasad Eye Institute, Hyderabad - 500 034, Telangana
India

Abstract

Purpose: The purpose was to study the efficacy of interferon alpha 2b (INF α2b) in the treatment of ocular surface squamous neoplasia (OSSN) and analyze its cost-effectiveness in India. Study Design: This was a retrospective study of thirty patients with OSSN treated with topical INF α2b (1 MIU/cc) ± perilesional INF α2b (5 MIU/cc). Results: The tumor involved cornea (n = 9, 30%), conjunctivo-limbal-corneal surface (n = 19, 63%), or bulbar conjunctiva (n = 2, 7%). The mean basal dimension of the tumor was 16 mm. The tumors belonged to Tis (n = 6, 20%) or T3 (n = 24, 80%) based on the American Joint Committee Classification, 7th edition. In the six patients with Tis, three cycles of topical INF α2b were used for immunoprevention. In the remaining 24 patients, INF α2b was advised for immunoreduction, but served as immunotherapy with 100% tumor regression in 22 (92%) cases, and resulted in 95% immunoreduction in 2 (6%) cases. Complete tumor regression by immunotherapy (n = 22) was achieved with a mean number of three topical INF α2b cycles and two perilesional injections. All these 22 patients received three additional topical INF α2b cycles after complete tumor regression. For immunoreduction (n = 2), both patients received six cycles of topical INF α2b which was three perilesional INF α2b injections. The mean total treatment cost per patient with INF α2b was INR 9164 ($US 137). Based on maximum basal diameter of tumor at presentation, the mean total treatment cost per patient with INF α2b was INR 4866 ($US 73) for eyes with microscopic evidence of tumor residue (n = 6), INR 9607 ($US 143) for tumors ≤10 mm (n = 13), and INR 10,985 ($US 164) for tumors >10 mm (n = 11), with two patients needing additional surgical excision for complete tumor control. Conclusion: INF α2b can be used for immunoreduction, immunotherapy, or immunoprevention of OSSN. INF α2b is a cost-effective treatment modality for OSSN at an average total treatment cost of INR 9164 ($US 137) per patient.



How to cite this article:
Kaliki S, Singh S, Iram S, Tripuraneni D. Recombinant interferon alpha 2b for ocular surface squamous neoplasia: An efficient and cost-effective treatment modality in Asian Indian patients.Indian J Ophthalmol 2016;64:702-709


How to cite this URL:
Kaliki S, Singh S, Iram S, Tripuraneni D. Recombinant interferon alpha 2b for ocular surface squamous neoplasia: An efficient and cost-effective treatment modality in Asian Indian patients. Indian J Ophthalmol [serial online] 2016 [cited 2024 Mar 29 ];64:702-709
Available from: https://journals.lww.com/ijo/pages/default.aspx/text.asp?2016/64/10/702/195010


Full Text

Ocular surface squamous neoplasia (OSSN) includes a spectrum from mild/moderate/severe dysplasia to carcinoma in situ to invasive squamous cell carcinoma involving the conjunctiva and/or cornea.[1] The most widely practiced treatment strategy for OSSN includes wide excision biopsy with 3–4 mm tumor-free margin by “no-touch” technique for the conjunctival component, alcohol-treated limited superficial keratectomy for the corneal component, followed by adjunct double-freeze thaw cryotherapy to the conjunctival surgical margins and surface reconstruction.[2] In extensive invasive OSSN, plaque radiotherapy, external beam radiotherapy, extended enucleation, or anterior orbital exenteration may be needed.[3],[4],[5] Topical chemotherapy with mitomycin-C (MMC), cidofovir, 5-fluorouracil (5-FU), or immunotherapy with interferon alpha 2b (INF α2b) has been found to be effective in the treatment of noninvasive OSSN.[6],[7],[8],[9],[10],[11]

OSSN most commonly arises in the limbal region,[1] sometimes involving 360° limbus. Extensive surgical excision can result in limbal stem cell loss and ocular surface morbidity. Topical agents offer the advantage of delivering treatment not only to the affected ocular surface but also to the entire ocular surface, and thus potentially eliminating subclinical OSSN, and also diminish the risk of limbal stem cell deficiency and associated ocular surface morbidity.[6],[7],[8],[9],[10],[11],[12],[13],[14],[15],[16],[17],[18],[19],[20],[21],[22] Although the efficacy of topical medications on OSSN is comparable (80%–100%),[6],[7],[8],[9],[10],[11],[12],[13],[14],[15],[16],[17],[18],[19],[20],[21],[22] MMC has the highest reported rates of side effects (76%), followed by 5-FU (42%), and INF α2b is reported to have the least rates of side effects (15%).[18],[19] However, the cost of INF α2b ($US 175–225 per treatment) could limit its use in some patients.[13],[14],[20],[21] In this study, we evaluate the efficacy of recombinant human INF α2b in the treatment of OSSN and analyze its cost-effectiveness.

 Methods



This study is a retrospective study conducted at a single institution. The Institutional Review Board approval was obtained for the study. The medical records of all patients with OSSN treated with INF α2b were reviewed. The study period ranged from August 2013 to December 2015. The patients with extensive tumor (>20 mm basal dimension or >6 clock hours involvement of limbus, with flat tumor configuration), OSSN limited to the corneal surface, recurrent tumor postsurgical excision and/or MMC, and those with microscopic tumor residue postexcision biopsy were treated with INF α2b. Patients with OSSN treated with INF α2b with a minimal follow-up of 3 months were included in the study. The patients who were noncompliant to treatment, or those who were lost to follow-up, or have <3 months follow-up, or with ongoing/incomplete treatment were excluded from the study.

The demographic details (age, gender) were reviewed. Systemic history and past history were recorded. The recorded clinical features included tumor laterality, presenting complaints, duration of symptoms (months), tumor location and extent (conjunctiva, cornea, conjunctival-limbal-corneal surface), maximum basal tumor diameter (mm), number of limbal clock hours involved by the tumor, and tumor pattern (leukoplakic, gelatinous, nodular, papillary, placoid, pigmented). The primary tumor was classified based on the American Joint Committee Classification, 7th edition.[23] The diagnosis of OSSN was made by clinical findings. Histopathology confirmation of the diagnosis of OSSN before initiation of treatment with INF α2b was established by slide/block review in cases with a previous history of excision biopsy. Locoregional lymph node examination and medical examination to rule out locoregional or systemic metastasis of tumor were performed in all cases.

INF α2b was used for immunoreduction (to reduce the tumor size) or immunotherapy (as the sole treatment for tumor regression) or immunoprevention (to prevent tumor recurrence in those with histopathology evidence of residual tumor at margins postexcision biopsy). The patients with only corneal OSSN were advised topical INF α2b eye drops (1 MIU/cc) four times/day for immunoreduction/immunotherapy. The patients with conjunctival or conjunctivo-limbal-corneal lesions were given a combination of topical INF α2b eye drops (1 MIU/cc) four times/day and perilesional INF α2b injection (5 MIU/cc) once a month for immunoreduction/immunotherapy. Those patients with Tis, who had undergone excision biopsy of the lesion and showed carcinoma in situ at one or more surgical margins on histopathology review, were advised topical INF α2b eye drops (1 MIU/cc) four times/day for immunoprevention.

Recombinant human INF α2b (1 cc prefilled syringe of Intalfa 5 MIU, manufactured and marketed by Intas Pharmaceuticals Limited, India) was used in all patients. The cost of each syringe during the study period and at the time of manuscript preparation was INR 811 ($US 12). Topical drops were prepared under sterile conditions by adding 4 cc of distilled water to 1 cc of INF α2b 5 MIU, to achieve a dose of 1 MIU/cc. The medication was packed in a thermocol icebox and two bottles of 5 ml each were dispatched to the patient at each visit during the treatment period. The patient was instructed to store the drug in a refrigerator (2°C–8°C) and apply one drop four times/day. Under sterile conditions and topical anesthesia, perilesional INF α2b 5 MIU/cc was injected directly from the prefilled syringe as an outpatient procedure in suitable cases.

All patients were advised to use topical medication four times/day for 1 month. Every patient was reviewed once a month during the treatment period. Tumor details were recorded during each visit. If the patient had a residual tumor, the patient was advised to continue topical medication until complete tumor regression and 3 months beyond complete tumor regression. Topical INF α2b 1 MIU/cc drops were freshly prepared and dispatched every month as needed. The additional perilesional injection was repeated every month in suitable cases. After complete tumor regression had been achieved, topical drops were continued for another 3 months. If there were tumor residue after six cycles of topical INF α2b eye drops and three perilesional injections of INF α2b, the lesion was surgically excised. The histopathology details of excised tumor were recorded. All patients with Tis receiving INF α2b as immunoprevention were advised three cycles of topical INF α2b eye drops. After complete tumor regression had been achieved, the patients were reviewed once in 3 months in the first year and every 4 months in the second year.

The outcome was also analyzed based on maximum tumor dimension on presentation. The patients were divided into three groups; Group 1, with no clinical evidence of tumor (Tis, INF α2b used to prevent tumor recurrence in those with histopathology evidence of residual tumor at margins postexcision biopsy); Group 2, with maximum basal diameter of tumor <10 mm; Group 3, with maximum basal diameter of tumor >10 mm at presentation.

The number of cycles of INF α2b (topical drops ± perilesional injections) per patient was recorded. The total treatment cost of INF α2b per patient was recorded. The side effects of treatment and any event of tumor recurrence were noted.

 Results



During the study period, 254 patients with OSSN were examined. Of these, 38 (11%) patients were treated with INF α2b. Eight patients were excluded from the study due to noncompliance to treatment (n = 2), <3 months follow-up duration (n = 2), combination treatment with perilesional INF α2b injection and topical MMC (n = 2), and ongoing treatment (n = 2). Of the thirty patients included in this study, the mean age at presentation was 55 years (median: 57 years; range: 27–80 years). There were 20 (67%) males and 10 (33%) females. History of prior intervention was present in 13 (43%) patients, including excision biopsy (n = 12; 40%) and/or MMC (n = 3; 10%). The most common presenting complaint included mass on the ocular surface (n = 18; 60%). The mean duration of symptoms was 12 months (median: 7 months; range: 1–48 months).

The tumor involved cornea (n = 9, 30%), conjunctivo-limbal-corneal surface (n = 19, 63%), or bulbar conjunctiva (n = 2, 7%). The mean basal dimension of the tumor was 16 mm (median: 20 mm; range: 4–30 mm). The mean number of limbal clock hours involved by the tumor was 6 (median: 5; range: 1–12). At the time of initiation of INF α2b, the tumors belonged to Tis (n = 6, 20%) or T3 (n = 24, 80%) [Table 1]. Histopathology confirmation of the diagnosis of OSSN was available in 12 (40%) cases before initiation of treatment with INF α2b.{Table 1}

Of the six patients with Tis, four patients had undergone excision biopsy of the lesion at another hospital and were referred to us with residual tumor at the surgical margins, and two patients were operated at our institute and histopathology showed positive margins. Carcinoma in situ at one or more surgical margins was noted on histopathology. These six patients were advised three cycles of topical INF α2b for immunoprevention. In the remaining 24 patients, INF α2b was advised for immunoreduction but served as sole immunotherapy with 100% tumor regression in 22 (92%) cases [Table 2].{Table 2}

Complete tumor regression by immunotherapy (n = 22) was achieved with a mean number of topical INF α2b cycles of 3 (median: 2; range: 1–5), and perilesional INF α2b injections of 2 (median: 2; range: 2–3). All these 22 patients with immunotherapy received three additional topical INF α2b cycles after complete tumor regression [Table 3] and [Figure 1] and [Figure 2]. For immunoreduction (n = 2), the mean number of topical INF α2b cycles was 6 (median: 6; range: 5–6), and perilesional INF α2b injections were 3 (median: 3; range: 3). The residual tumor was surgically excised. On histopathology, both patients showed mild dysplasia.{Table 3}{Figure 1}{Figure 2}

The mean total treatment cost per patient (including additional three cycles of topical INF α2b after complete tumor regression) with INF α2b was INR 5001 ($US 75) (median: INR 4866 [$US 73]; range: 2433 [$US 36]–8921 [$US 133]) [Table 3]. The side effects of treatment with INF α2b included mild conjunctival hyperemia (n = 4, 13%), which was temporary and resolved after discontinuation of treatment, and flu-like syndrome (n = 1, 8%), which was present in the first 6 h of perilesional INF α2b injection, and subsided with a single dose of oral acetaminophen. No tumor recurrence was noted in any patient at a mean follow-up period of 9 months (median: 7 months; range: 3–28 months).

Based on maximum tumor dimension at presentation [Table 4], the mean number of topical and injection INF α2b cycles was 3 and 0 for Group 1, 5 and 2 for Group 2, and 6 and 3 for Group 3, respectively. The mean total treatment cost per patient (including additional three cycles of topical INF α2b after complete tumor regression) with INF α2b was INR 4866 ($US 73) for Group 1, INR 9607 ($US 143) for Group 2, and INR 10,985 ($US 164) for Group 3. Two patients had minimal tumor residue (5% residual tumor) with INF α2b and underwent surgical excision of the residual tumor.{Table 4}

 Discussion



INF α2b is a Type 1 INF consisting of 165 amino acid residues with arginine in position 23. This glycoprotein is produced by recombinant DNA technology and resembles INF secreted by leukocytes.[18],[19] It exhibits antineoplastic and antiviral effects.[18],[19] In solid tumors, INF α causes prolongation of the cell cycle time of malignant cells, inhibits biosynthetic enzymes and apoptosis, interacts with other cytokines, and increases the immunomodulatory and antiangiogenic effects.[24] In cutaneous basal and squamous cell carcinomas, INF α causes tumor regression by concomitant upregulation of interleukin (IL)-2 and downregulation of IL-10 messenger RNA expression in the lesions, thus increasing immunomodulatory effects by improving the T-cell mediated immune response.[25] The mechanism of action of INF α2b in OSSN is unclear but may include inhibition of angiogenesis and/or inhibition of human papillomavirus replication.[26],[27] However, recent studies have shown that the presence of human papillomavirus is not required for a favorable response with INF α2b.[28]

The US Food and Drug Administration has approved INF α2b for the treatment of AIDS-related Kaposi sarcoma, hairy cell leukemia, malignant melanoma, aggressive follicular non-Hodgkin's lymphoma, chronic hepatitis B and C, and condyloma acuminata. INF α2b has been used off-label in the treatment of OSSN since the first publication in 1994.[22] Good response to treatment with complete tumor regression is achieved in 85%–100% OSSN cases with INF α2b.[12],[13],[14],[15],[16],[17],[18],[19],[20],[21],[22]

In a study of 22 cases of OSSN treated with INF α2b monotherapy, complete tumor regression was achieved in 75% Tis (three of four patients), 100% T1 (all eight patients), and 70% T3 (seven of ten patients).[14] In our study, complete tumor regression was achieved in 100% Tis (all six patients) and 92% T3 (22 of 24 patients). INF α2b with/without surgery achieved complete tumor regression in 100% cases. Complete tumor control was achieved with topical INF α2b monotherapy in 15 (50%) cases, a combination of topical and perilesional injection INF α2b in 13 (43%), and a combination of surgery and INF α2b in 2 (7%) cases.

INF α2b can be used for immunoreduction in extensive lesions (tumor basal diameter of 20 mm or >6 clock-hours),[13],[17],[20],[29] as immunotherapy in corneal lesions or smaller conjunctival or conjunctivo-corneal lesions (tumor basal diameter <20 mm and <6 clock-hours),[21],[30],[31] and for immunoprevention in patients with histopathology evidence of residual tumor.[14] In a study of 18 patients with extensive OSSN by Kim et al., INF α2b served as immunotherapy achieving complete tumor regression in 13 (72%) patients and achieved immunoreduction in 28% cases by a median duration of 6 months.[20] In a study of five patients by Karp et al., two patients had corneal OSSN and both showed complete tumor regression with topical INF α2b as immunotherapy by a median duration of 2 months.[16] In our study, there were 15 (50%) patients with extensive lesions, 9 (30%) with corneal lesions, and 6 (20%) patients with a residual tumor on histopathology. Of the 15 patients with extensive lesions, INF α2b served as immunotherapy achieving complete tumor regression in 13 (87%) patients at a median duration of 3 months and achieved 95% immunoreduction in 2 (13%) patients at a median duration of 6 months. Of the nine patients with corneal OSSN, topical INF α2b as immunotherapy achieved complete tumor regression in 100% cases at a median duration of 2 months. Similar to prior studies, we found that corneal OSSN responded earlier to treatment with INF α2b.[16],[21] The earlier response could be attributed to placoid nature of corneal tumor with minimal tumor thickness.

As per published literature, topical INF α2b is effective at a dose of 1 MIU/cc four to six times/day.[14],[15],[16],[17],[18],[19],[20],[21],[22],[29],[30],[31],[32] A comparison of effectiveness and side effect profile of two doses of topical INF α2b (1 MIU/cc vs. 3 MIU/cc) in the treatment of OSSN showed no significant difference between the two doses.[33] However, there is no consensus on the dose and dosage of perilesional INF. Some authors use 3 MIU/0.5 cc one to three times a week until clinical resolution [13],[17],[32] while others use 10 MIU/cc once a month until clinical resolution.[14],[20],[29] In our study, the dose and dosage of topical INF α2b was 1 MIU/cc four times a day until clinical resolution, and the dose of perilesional INF α2b was 5 MIU/cc once a month until clinical resolution of conjunctival component of the tumor. Furthermore, there is a lack of consensus on the duration of continuation of topical INF α2b after tumor resolution and ranges from 1 to 4 months.[14],[15],[16],[30],[31],[33] In our study, all patients were advised to continue topical INF α2b for 3 months beyond clinical tumor resolution.

The cost of treatment with INF α2b is three times more than 5-FU and two times more than MMC.[34] The cost of each 10 MIU/cc vial of INF α2b is $US 179 (Jefferson Pharmacy; Philadelphia, PA, USA),[20],[21] and it can be used to prepare 10 cc of 1 MIU/cc topical INF α2b drops for 1 month. The cost for 6 months would be $US 1074. The cost of 5 ml of 1 MIU is $US 120 (Leiter's Pharmacy, San Jose, CA, USA),[33] thus costing $US 240 for 10 cc of 1 MIU/cc topical INF α2b drops for 1 month. The cost for 6 months would be $US 1440. For perilesional injection, the patient has to bear an additional cost of $179 to $US 225 (Leiter's Pharmacy, San Jose, CA, USA) per injection, thus further increasing the total treatment cost.[13] In our study, each syringe of Intalfa 5 MIU/cc costs INR 811 ($US 12). An average six cycles of topical INF α2b (with 10 cc of 1 MIU/cc dispatched each month) costs INR 9732 ($US 144), with an additional cost of INR 811 ($US 12) per perilesional injection. In our study, the average total treatment cost per patient with INF α2b (topical with/without perilesional injection) was INR 9164 ($US 137), thus making it cost-effective for our patients. Two patients had to undergo surgical excision of the residual tumor, thus resulting in additional costs.

There is no difference in the recurrence rate of OSSN at 1-year between surgical excision (5%) and medical treatment with INF α2b (3%).[15] In our study, no tumor recurrence was noted in any patient at a mean follow-up period of 9 months (median: 7 months; range: 3–28 months). INF α2b has fewer side effects than other topical agents used in OSSN. Ocular side effects include conjunctival hyperemia (5%), ocular irritation (4%), superficial punctate keratitis (4%), and follicular conjunctivitis (1%).[14] Systemic side effects include postinjection flu-like syndrome for 1 day (9%).[14] In our study, the side effects included transient conjunctival hyperemia (13%) and flu-like syndrome (8%), which were comparable with other studies.[13],[14],[33]

The limitations of the study include retrospective nature of the study, lack of histopathologic documentation of diagnosis in all cases, smaller patient cohort, and shorter duration of follow-up. The cost-effectiveness of treatment has been calculated by taking into account only the cost of INF α2b syringe. However, the patient has additional costs of frequent follow-up visits since topical INF α2b drops needs to be freshly reconstituted each month and stored at appropriate temperature in a refrigerator. The number of follow-up visits with INF α2b medical treatment is more compared to primary surgery. However, the total cost of treatment with INF α2b medical treatment is less compared to the surgical group.[35] In addition, medical treatment also avoids additional surgeries for limbal stem cell deficiency resulting from extensive surgical excision and cryotherapy for large lesions.

 Conclusion



INF α2b is an effective alternative to surgery in suitable cases and can be used for immunoreduction, immunotherapy, or immunoprevention of OSSN. While surgical tumor excision is still the most commonly used treatment modality at our center, INF α2b is reserved for patients with extensive tumor (>20 mm basal dimension or >6 clock hours involvement of limbus with flat tumor configuration), OSSN limited to the corneal surface, recurrent tumor postsurgical excision and/or MMC, and those with microscopic tumor residue postexcision biopsy. In our study, INF α2b was a cost-effective treatment modality for OSSN at an average total treatment cost of INR 9164 ($US 137) per patient.

Financial support and sponsorship

This study was supported by the Operation Eyesight Universal Institute for Eye Cancer and Hyderabad Eye Research Foundation, Hyderabad.

Conflicts of interest

There are no conflicts of interest.

References

1Lee GA, Hirst LW. Ocular surface squamous neoplasia. Surv Ophthalmol 1995;39:429-50.
2Shields JA, Shields CL, De Potter P. Surgical management of conjunctival tumors. The 1994 Lynn B. McMahan Lecture. Arch Ophthalmol 1997;115:808-15.
3Walsh-Conway N, Conway RM. Plaque brachytherapy for the management of ocular surface malignancies with corneoscleral invasion. Clin Exp Ophthalmol 2009;37:577-83.
4Arepalli S, Kaliki S, Shields CL, Emrich J, Komarnicky L, Shields JA. Plaque radiotherapy in the management of scleral-invasive conjunctival squamous cell carcinoma: An analysis of 15 eyes. JAMA Ophthalmol 2014;132:691-6.
5Shields JA, Shields CL, Suvarnamani C, Tantisira M, Shah P. Orbital exenteration with eyelid sparing: Indications, technique, and results. Ophthalmic Surg 1991;22:292-7.
6Shields CL, Naseripour M, Shields JA. Topical mitomycin C for extensive, recurrent conjunctival-corneal squamous cell carcinoma. Am J Ophthalmol 2002;133:601-6.
7Singh AD, Jacques R, Rundle PA, Rennie IG, Mudhar HS, Slater D. Neoadjuvant topical mitomycin C chemotherapy for conjunctival and corneal intraepithelial neoplasia. Eye (Lond) 2006;20:1092-4.
8Midena E, Angeli CD, Valenti M, de Belvis V, Boccato P. Treatment of conjunctival squamous cell carcinoma with topical 5-fluorouracil. Br J Ophthalmol 2000;84:268-72.
9Yeatts RP, Engelbrecht NE, Curry CD, Ford JG, Walter KA. 5-Fluorouracil for the treatment of intraepithelial neoplasia of the conjunctiva and cornea. Ophthalmology 2000;107:2190-5.
10Yeatts RP, Ford JG, Stanton CA, Reed JW. Topical 5-fluorouracil in treating epithelial neoplasia of the conjunctiva and cornea. Ophthalmology 1995;102:1338-44.
11Sherman MD, Feldman KA, Farahmand SM, Margolis TP. Treatment of conjunctival squamous cell carcinoma with topical cidofovir. Am J Ophthalmol 2002;134:432-3.
12Besley J, Pappalardo J, Lee GA, Hirst LW, Vincent SJ. Risk factors for ocular surface squamous neoplasia recurrence after treatment with topical mitomycin C and interferon alpha-2b. Am J Ophthalmol 2014;157:287-93.e2.
13Karp CL, Galor A, Chhabra S, Barnes SD, Alfonso EC. Subconjunctival/perilesional recombinant interferon a2b for ocular surface squamous neoplasia: A 10-year review. Ophthalmology 2010;117:2241-6.
14Shields CL, Kaliki S, Kim HJ, Al-Dahmash S, Shah SU, Lally SE, et al. Interferon for ocular surface squamous neoplasia in 81 cases: Outcomes based on the American Joint Committee on Cancer classification. Cornea 2013;32:248-56.
15Nanji AA, Moon CS, Galor A, Sein J, Oellers P, Karp CL. Surgical versus medical treatment of ocular surface squamous neoplasia: A comparison of recurrences and complications. Ophthalmology 2014;121:994-1000.
16Karp CL, Moore JK, Rosa RH Jr. Treatment of conjunctival and corneal intraepithelial neoplasia with topical interferon alpha-2b. Ophthalmology 2001;108:1093-8.
17Vann RR, Karp CL. Perilesional and topical interferon alfa-2b for conjunctival and corneal neoplasia. Ophthalmology 1999;106:91-7.
18Sepulveda R, Pe'er J, Midena E, Seregard S, Dua HS, Singh AD. Topical chemotherapy for ocular surface squamous neoplasia: Current status. Br J Ophthalmol 2010;94:532-5.
19Poothullil AM, Colby KA. Topical medical therapies for ocular surface tumors. Semin Ophthalmol 2006;21:161-9.
20Kim HJ, Shields CL, Shah SU, Kaliki S, Lally SE. Giant ocular surface squamous neoplasia managed with interferon alpha-2b as immunotherapy or immunoreduction. Ophthalmology 2012;119:938-44.
21Shah SU, Kaliki S, Kim HJ, Lally SE, Shields JA, Shields CL. Topical interferon alfa-2b for management of ocular surface squamous neoplasia in 23 cases: Outcomes based on American Joint Committee on Cancer classification. Arch Ophthalmol 2012;130:159-64.
22Maskin SL. Regression of limbal epithelial dysplasia with topical interferon. Arch Ophthalmol 1994;112:1145-6.
23Edge SB, Byrd DR, Compton CC, Fritz AG, Greene FL, Trotti A 3rd, editors. Carcinoma of the conjunctiva. In: AJCC Cancer Staging Manual. 7th ed. New York, Dordrecht, Heidelberg, London: Springer; 2010. p. 531-7.
24Decatris M, Santhanam S, O'Byrne K. Potential of interferon-alpha in solid tumours: Part 1. BioDrugs 2002;16:261-81.
25Kim J, Modlin RL, Moy RL, Dubinett SM, McHugh T, Nickoloff BJ, et al. IL-10 production in cutaneous basal and squamous cell carcinomas. A mechanism for evading the local T cell immune response. J Immunol 1995;155:2240-7.
26Majewski S, Szmurlo A, Marczak M, Jablonska S, Bollag W. Synergistic effect of retinoids and interferon alpha on tumor-induced angiogenesis: Anti-angiogenic effect on HPV-harboring tumor-cell lines. Int J Cancer 1994;57:81-5.
27Gangemi JD, Pirisi L, Angell M, Kreider JW. HPV replication in experimental models: Effects of interferon. Antiviral Res 1994;24:175-90.
28Galor A, Garg N, Nanji A, Joag M, Nuovo G, Palioura S, et al. Human papilloma virus infection does not predict response to interferon therapy in ocular surface squamous neoplasia. Ophthalmology 2015;122:2210-5.
29Shields CL, Kancherla S, Bianciotto CG, Lally SE, Shields JA. Ocular surface squamous neoplasia (squamous cell carcinoma) of the socket: Management of extensive tumors with interferon. Ophthal Plast Reconstr Surg 2011;27:247-50.
30Schechter BA, Schrier A, Nagler RS, Smith EF, Velasquez GE. Regression of presumed primary conjunctival and corneal intraepithelial neoplasia with topical interferon alpha-2b. Cornea 2002;21:6-11.
31Schechter BA, Koreishi AF, Karp CL, Feuer W. Long-term follow-up of conjunctival and corneal intraepithelial neoplasia treated with topical interferon alfa-2b. Ophthalmology 2008;115:1291-6.
32Nemet AY, Sharma V, Benger R. Interferon alpha 2b treatment for residual ocular surface squamous neoplasia unresponsive to excision, cryotherapy and mitomycin-C. Clin Exp Ophthalmol 2006;34:375-7.
33Galor A, Karp CL, Chhabra S, Barnes S, Alfonso EC. Topical interferon alpha 2b eye-drops for treatment of ocular surface squamous neoplasia: A dose comparison study. Br J Ophthalmol 2010;94:551-4.
34Esquenazi S, Fry CL, Holley E. Treatment of biopsy proved conjunctival intraepithelial neoplasia with topical interferon alfa-2b. Br J Ophthalmol 2005;89:1221.
35Moon CS, Nanji AA, Galor A, McCollister KE, Karp CL. Surgical versus medical treatment of ocular surface squamous neoplasia: A cost comparison. Ophthalmology 2016;123:497-504.