Indian Journal of Ophthalmology

OPHTHALMIC IMAGE
Year
: 2020  |  Volume : 68  |  Issue : 10  |  Page : 2252-

Corneal epithelial cells division assessed by scanning electron microscopy


Carlos Rocha-de-Lossada, Josep Torras-Sanvicens, Jorge Peraza-Nieves 
 Department of Corneal and Anterior Segment, Hospital Clinic of Barclona, Barcelona, Spain

Correspondence Address:
Dr. Carlos Rocha-de-Lossada
Hospital Clinic of Barcelona, Barcelona
Spain




How to cite this article:
Rocha-de-Lossada C, Torras-Sanvicens J, Peraza-Nieves J. Corneal epithelial cells division assessed by scanning electron microscopy.Indian J Ophthalmol 2020;68:2252-2252


How to cite this URL:
Rocha-de-Lossada C, Torras-Sanvicens J, Peraza-Nieves J. Corneal epithelial cells division assessed by scanning electron microscopy. Indian J Ophthalmol [serial online] 2020 [cited 2024 Mar 28 ];68:2252-2252
Available from: https://journals.lww.com/ijo/pages/default.aspx/text.asp?2020/68/10/2252/295662


Full Text



Scanning electron microscopy (SEM) is a technique for obtaining high resolution images of biological and non-biological specimens. SEM has been used in the characterization of the surface of human corneo-conjunctival anatomy.[1]

The corneal epithelial cells divide in a rapid turnover to maintain the ocular surface homeostasis. Corneal epithelial cells are constantly replenished by limbal epithelial stem cells (LESC), after differentiation, they can proliferate by mitosis and migrate centripetally. Alterations to limbal environment can result in LESC dysfunction.[2]

Our aim is to show the ultrastructure by SEM of the corneal epithelial cells during mitotic division above the epithelial basal membrane. The cells are edematous in profile and intermediate in brightness, with multiple microprojections of different shapes, sizes, and numbers (microplicae and microvilli) [Figure 1]a and [Figure 1]b.{Figure 1}

Financial support and sponsorship

Nil.

Conflicts of interest

There are no conflicts of interest.

References

1Versura P, Bonvicini F, Caramazza R, Laschi R. Scanning electron microscopy study of human cornea and conjunctiva in normal and various pathological conditions. Scan Electron Microsc. 1985;(Pt 4):1695-1708.
2Zhao Y, Ma L. Systematic review and meta-analysis on transplantation of ex vivo cultivated limbal epithelial stem cell on amniotic membrane in limbal stem cell deficiency. Cornea 2015;34:592-600.