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Figure 2: (1) Toxoplasma incubated with `fresh normal serum' and then mixed with methylene blue. Note that the organisms are rounded or oval, the cytoplasm stains dark, and the lighter-staining chromatin body is surrounded by a light zone. Arrow points to a group of intracellular Toxoplasma. Different fields are presented because of difficulty' of getting organisms into focus in wet preparation. (x650.) (2) Toxoplasma incubated with `fresh immune serum' and then mixed with methrlene blue. Note that the organisms are mostly crescentic, the cytoplasm is unstained except in the intracellular group (arrow), and the chromatin remains stained. Wet preparation. (x650.) |
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