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Figure 1: Case 1 (a) Slit-lamp picture showing central stromal infiltrate and hypopyon in diffuse illumination, (b) potassium hydroxide and calcofluor white wet mount of corneal scraping from a patient with microsporidial keratitis showing numerous microsporidial spores under a fluorescence microscope (original magnification × 400). (c) Low-magnification photomicrograph showing ulcerated corneal epithelium and surface covered by fibrin glue. There is diffuse stromal necrosis with loss of lamellar arrangement of stromal collagen. There is diffuse infiltration of polymorphonuclear leukocytes of severe degree admixed with debris material (H and E, ×100). (d) High-magnification photomicrograph shows granulomatous inflammation in the posterior stroma and along Descemet's membrane. Numerous histiocytes are seen with pale staining abundant cytoplasm and round to oval peripheral nuclei (arrow marked). Histiocytes are seen coalescing to form multinucleated giant cells (asterisk marked) (H and E, ×400). (e and f) Representative gel pictures of pan-microsporidial polymerase chain reaction (arrow marked in e) and Herpes simplex virus-1 polymerase chain reaction (arrow marked in f) showing amplification of 1200 base pair and 221 base pair products in patient sample number 223/11 respectively. NC: Negative control, PC: Positive control and MW– 100 base pair plus molecular weight DNA ladder |
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